A5006284431- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- Marine Biology and Ecology Research
- Metabolomics and Mass Spectrometry Studies
- Isotope Analysis in Ecology
- Marine Bivalve and Aquaculture Studies
- Bacteriophages and microbial interactions
- Methane Hydrates and Related Phenomena
- Photoreceptor and optogenetics research
- Microbial bioremediation and biosurfactants
- Wastewater Treatment and Nitrogen Removal
- Indigenous Studies and Ecology
- Gene expression and cancer classification
- Light effects on plants
- Diatoms and Algae Research
- Coral and Marine Ecosystems Studies
University of Florida
2020-2024
University of Washington
2018-2022
University of Georgia
2012-2017
University of Hawaii System
2014
Significance In the surface ocean, organic matter released by phytoplankton and degraded heterotrophic bacteria is a key step in carbon cycle. Compounds important this trophic link are poorly known, part because of thousands chemicals making up marine dissolved matter. We cocultured Roseobacter clade bacterium with diatom Thalassiosira pseudonana used gene expression changes to assay for compounds passed bacterium. A C 3 -sulfonate no previously known role microbial food web was identified...
Sunlight is the dominant control on phytoplankton biosynthetic activity, and darkness deprives them of their primary external energy source. Changes in biochemical composition communities over diel light cycles attendant consequences for carbon flux environments remain poorly elucidated. Here we use lipidomic data from North Pacific subtropical gyre to show that biosynthesis energy-rich triacylglycerols (TAGs) by eukaryotic nanophytoplankton during day subsequent consumption at night drives...
The trophic linkage between marine bacteria and phytoplankton in the surface ocean is a key step global carbon cycle, with almost half of primary production transformed by heterotrophic bacterioplankton within hours to weeks fixation. Early studies conceptualized this link as passive addition removal organic compounds from shared seawater reservoir. Here, we analysed transcript intracellular metabolite patterns two-member model system found that presence bacterium induced potential...
Intricate networks of single-celled eukaryotes (protists) dominate carbon flow in the ocean. Their growth, demise, and interactions with other microorganisms drive fluxes biogeochemical elements through marine ecosystems. Mixotrophic protists are capable both photosynthesis ingestion prey dominant components open-ocean planktonic communities. Yet role mixotrophs elemental cycling is obscured by their capacity to act as primary producers or heterotrophic consumers depending on factors that...
Abstract The photosynthetic picocyanobacteria Prochlorococcus and Synechococcus are models for dissecting how ecological niches defined by environmental conditions, but interactions with bacteriophages affect picocyanobacterial biogeography in open ocean biomes has rarely been assessed. We applied single-virus single-cell infection approaches to quantify cyanophage abundance infected 87 surface water samples from five transects that traversed approximately 2,200 km the North Pacific Ocean on...
Abstract Siderophores are strong iron‐binding molecules produced and utilized by microbes to acquire the limiting nutrient iron (Fe) from their surroundings. Despite importance as a component of ligand pool in seawater, data on distribution siderophores that use them limited. Here, we measured concentrations types dissolved during two cruises April 2016 June 2017 transited iron‐replete, low‐macronutrient North Pacific Subtropical Gyre through Transition Zone (NPTZ) iron‐deplete,...
Glycine betaine (GBT) is a compatible solute in high concentrations marine microorganisms. As component of labile organic matter, GBT has complex biochemical potential as substrate for microbial use that unconstrained the environment. Here we determine uptake kinetics and metabolic fate two natural communities North Pacific characterized by different nitrate concentrations. Dissolved had maximum rates 0.36 0.56 nM h-1 with half-saturation constants 79 11 low stations respectively. During...
Light fuels photosynthesis and organic matter production by primary producers in the sunlit ocean. The quantity quality of produced influence community function, yet situ measurements metabolites, products cellular metabolism, over diel cycle are lacking. We evaluated community-level biochemical consequences oscillations light North Pacific Subtropical Gyre quantifying 79 metabolites particulate from 15 m every 4 h 8 days. Total metabolite concentration peaked at dusk represented up to 2%...
The 24-h cycle of light and darkness governs daily rhythms complex behaviors across all domains life. Intracellular photoreceptors sense specific wavelengths that can reset the internal circadian clock and/or elicit distinct phenotypic responses. In surface ocean, microbial communities additionally modulate nonrhythmic changes in quality quantity as they are mixed to different depths. Here, we show eukaryotic plankton North Pacific Subtropical Gyre transcribe genes encoding light-sensitive...
Phytoplankton transform inorganic carbon into thousands of biomolecules that represent an important pool fixed carbon, nitrogen, and sulfur in the surface ocean. Metabolite production differs between phytoplankton, flux these molecules through microbial food web depends on compound-specific bioavailability to members a wider community. Yet relatively little is known about diversity or concentration metabolites within marine plankton. Here, we compare 313 polar 21 cultured phytoplankton...
Strain HIMB11 is a planktonic marine bacterium isolated from coastal seawater in Kaneohe Bay, Oahu, Hawaii belonging to the ubiquitous and versatile Roseobacter clade of alphaproteobacterial family Rhodobacteraceae. Here we describe preliminary characteristics strain HIMB11, including annotation draft genome sequence comparative genomic analysis with other members lineage. The 3,098,747 bp arranged 34 contigs contains 3,183 protein-coding genes 54 RNA genes. Phylogenomic 16S rRNA gene...
Most biological diversity on Earth is contained within microbial communities. In the ocean, these communities dominate processes related to carbon fixation and nutrient recycling. Yet, specific factors that determine community composition metabolic activity are difficult resolve in complex populations, complicating predictions of a changing ocean. Microbial metabolism generates small organic molecules reflect both biochemical physiological as well taxonomic specificity processes. These serve...
Abstract Light is the primary input of energy into sunlit ocean, driving daily oscillations in metabolism producers. The consequences this solar forcing have implications for whole microbial community, yet situ measurements metabolites, direct products cellular activity, over diel cycle are scarce. We evaluated community-level biochemical North Pacific Subtropical Gyre by quantifying 79 metabolites particulate organic matter surface waters every four hours eight days. Total metabolite...
Abstract Siderophores are strong iron-binding molecules produced and utilized by microbes to acquire the limiting nutrient iron (Fe) from their surroundings. Despite importance as a component of ligand pool in seawater, data on distribution siderophores that use them limited. Here we measured concentrations types dissolved during two cruises April 2016 June 2017 transited iron-replete, low-macronutrient North Pacific Subtropical Gyre (NPSG) through Transition Zone (NPTZ) iron-deplete,...
Microbes are omnipresent in the biosphere and perform biological chemical processes critical to ecosystem function, nutrient cycling, global climate regulation. In ocean, microbes constitute more than two-thirds of biomass with abundances reaching over one million microbial cells per milliliter seawater. Our understanding marine world has rapidly expanded use innovative molecular 'omics tools uncover previously hidden taxonomic diversity, spatiotemporal distributions, novel metabolic...
Open-ocean surface waters host a diverse community of single-celled eukaryotic plankton (protists) consisting phototrophs, heterotrophs, and mixotrophs. The productivity biomass these organisms oscillate over diel cycles, yet the underlying transcriptional processes are known for few members community. Here, we examined 4-day time series abundance profiles protist (0.2–100 μm in cell size) North Pacific Subtropical Gyre near Station ALOHA. De novo assembly poly-A+ selected metatranscriptomes...
Marine microbial eukaryotes (protists) perform essential metabolic functions in oceanic ecosystems. The diversity of protist remains poorly understood as few species have been isolated laboratory settings. Metatranscriptomes provide an invaluable tool for exploring and genetic capacities within their natural habitats. Here, we introduce the North Pacific Eukaryotic Gene Catalog, a compilation metatranscriptome data derived from total 261 metatranscriptomes: 169 metatranscriptomes were...
ABSTRACT Phytoplankton transform inorganic carbon into thousands of biomolecules that represent an important pool fixed carbon, nitrogen, and sulfur in the surface ocean. Metabolite production differs between phytoplankton, flux these molecules through microbial food web depends on compound-specific bioavailability to members a wider community. Yet relatively little is known about diversity or concentration metabolites within marine plankton. Here we compare 313 polar 21 cultured...