A5061919132- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Plant and animal studies
- Genetic Neurodegenerative Diseases
- Advanced biosensing and bioanalysis techniques
- Plant Reproductive Biology
- Cardiomyopathy and Myosin Studies
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Fungal and yeast genetics research
- Plant Parasitism and Resistance
- Chromosomal and Genetic Variations
Tianjin University
2024
Zhejiang Cancer Hospital
2024
Chinese Academy of Sciences
2022-2024
Institute of Botany
2022-2023
China National Botanical Garden
2023
University of Chinese Academy of Sciences
2022-2023
Beijing Botanical Garden
2023
The recent advent of single-cell RNA sequencing (scRNA-seq) has enabled access to the developmental landscape a complex organ by monitoring differentiation trajectory every specialized cell type at level. A main challenge in this endeavor is dissociating plant cells from rigid walls and some species are recalcitrant such cellular isolation. Here, we describe establishment simple efficient protocol for protoplast preparation Chirita pumila, which includes two consecutive digestion processes...
Abstract An expansion of AAGGG pentanucleotide repeats in the replication factor C subunit 1 (RFC1) gene is genetic cause cerebellar ataxia, neuropathy, and vestibular areflexia syndrome (CANVAS), it also links to several other neurodegenerative diseases including Parkinson's disease. However, pathogenic mechanism RFC1 repeat remains enigmatic. Here, we report that form DNA RNA parallel G-quadruplex (G4) structures play a role impairing biological processes. We determine first...
Abstract Actinomorphic flowers usually orient vertically (relative to the horizon) and possess symmetric nectar guides, while zygomorphic often face horizontally have asymmetric indicating that floral symmetry, orientation, guide patterning are correlated. The origin of zygomorphy is dependent on dorsoventrally expression CYCLOIDEA (CYC)-like genes. However, how horizontal orientation guides achieved remains poorly understood. Here, we selected Chirita pumila (Gesneriaceae) as a model plant...
Abstract A pentanucleotide repeat expansion (PRE) of (AAGGG) n in the replication factor C subunit 1 ( RFC1 ) gene is recently identified as genetic cause cerebellar ataxia, neuropathy, and vestibular areflexia syndrome (CANVAS), also linked to several other neurodegenerative disorders including Parkinson’s disease. However, molecular mechanism by which PRE drives pathology remains poorly understood. Here, for first time we discovered determined high-resolution structures parallel...
Myotonic dystrophy type 2 (DM2) is a neurogenerative disease caused by caprylic/capric triglyceride (CCTG) tetranucleotide repeat expansions in intron 1 of the cellular nucleic acid‐binding protein ( CNBP ) gene. Non‐B DNA structures formed CCTG repeats can promote genetic instability, whereas interrupting motifs NCTG (N = A/T/G) within help to maintain genomic stability. However, whether affect remains unclear. Here, we report that four with an 3′‐A/T/G residue dumbbell structures,...