A5055751443- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Muscle metabolism and nutrition
- Muscle Physiology and Disorders
- Muscle activation and electromyography studies
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Exercise and Physiological Responses
University of Jyväskylä
2021-2024
Abstract Background Proliferating cancer cells shift their metabolism towards glycolysis, even in the presence of oxygen, to especially generate glycolytic intermediates as substrates for anabolic reactions. We hypothesize that a similar metabolic remodelling occurs during skeletal muscle hypertrophy. Methods used mass spectrometry hypertrophying C2C12 myotubes vitro and plantaris mouse vivo assessed metabolomic changes incorporation [U‐ 13 C 6 ]glucose tracer. performed enzyme inhibition...
Cells use glycolytic intermediates for anabolism, e.g., via the serine synthesis and pentose phosphate pathways. However, we still understand poorly how these metabolic pathways contribute to skeletal muscle cell biomass generation. The first aim of this study was therefore identify enzymes that limit protein synthesis, myotube size, proliferation in cells. We inhibited key glycolysis, pathway, pathway evaluate their importance C2C12 synthesis. Based on results screen, then focused enzyme...
Impaired lipid metabolism is a common risk factor underlying several metabolic diseases such as syndrome and type 2 diabetes. Branched-chain amino acids (BCAAs) that include valine, leucine isoleucine have been proven to share role in hence maintaining health. We previously introduced hypothesis suggesting BCAA degradation mechanistically connects oxidation storage skeletal muscle. To test our hypothesis, the present study examined effects of deprivation supplementation on oxidation,...
The application of exercise-like electrical pulse simulation (EL-EPS) has become a widely used exercise mimetic in vitro. EL-EPS produces similar physiological responses as vivo exercise, while less is known about the detailed metabolic effects. Routinely, C2C12 myotubes are cultured high-glucose medium (4.5 g/L), which may alter responses. In this study, we evaluate effects under high- and low-glucose (1.0 g/L) conditions to understand how substrate availability affects myotube response...
ABSTRACT Background Proliferating cancer cells shift their metabolism toward glycolysis even in the presence of oxygen to especially generate glycolytic intermediates as substrates for anabolic reactions. We hypothesize that a similar metabolic remodeling occurs during skeletal muscle hypertrophy. Methods used mass spectrometry hypertrophying C2C12 myotubes vitro and plantaris mice vivo assessed metabolomic changes incorporation [U- 13 C 6 ]glucose tracer. performed enzyme inhibition key...