A5003550163- Plant Molecular Biology Research
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Antioxidant Activity and Oxidative Stress
- Plant Pathogens and Fungal Diseases
- Light effects on plants
- Plant Pathogens and Resistance
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Plant Taxonomy and Phylogenetics
- Plant Virus Research Studies
- Microbial Metabolites in Food Biotechnology
- Plant Parasitism and Resistance
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Banana Cultivation and Research
- RNA Research and Splicing
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Transgenic Plants and Applications
- Genetic Mapping and Diversity in Plants and Animals
University of California, Berkeley
2009-2022
Harvard University
2009
Massachusetts General Hospital
2009
Plant (United States)
2004-2005
Integrated DNA Technologies (United States)
2002
Bananas (Musa spp.), including dessert and cooking types, are giant perennial monocotyledonous herbs of the order Zingiberales, a sister group to well-studied Poales, which include cereals. vital for food security in many tropical subtropical countries most popular fruit industrialized countries. The Musa domestication process started some 7,000 years ago Southeast Asia. It involved hybridizations between diverse species subspecies, fostered by human migrations, selection diploid triploid...
While prokaryotic pan-genomes have been shown to contain many more genes than any individual organism, the prevalence and functional significance of differentially present in eukaryotes remains poorly understood. Whole-genome de novo assembly annotation 54 lines grass Brachypodium distachyon yield a pan-genome containing nearly twice number found genome. Genes all are enriched for essential biological functions, while only some conditionally beneficial functions (e.g., defense development),...
Oropetium thomaeum is a resurrection plant that can survive extreme water stress through desiccation to complete dryness, providing model for drought tolerance; here, whole-genome sequencing and assembly of the genome using single-molecule real-time reported. endure dryness whilst retaining ability revive when available, tolerance. These authors report O. genome, only (SMRT) long-read sequencing. Understanding genomic mechanisms tolerance in plants such as may provide targets engineering...
Small RNAs trigger repressive DNA methylation at thousands of transposable elements in a process called RNA-directed (RdDM). The molecular mechanism RdDM is well characterized Arabidopsis, yet the biological function remains unclear, as loss Arabidopsis causes no overt defects, even after generations inbreeding. It known that 24 nucleotide Pol IV-dependent siRNAs, hallmark RdDM, are abundant flowers and developing seeds, indicating might be important during reproduction. Here we show that,...
Small RNAs are abundant in plant reproductive tissues, especially 24-nucleotide (nt) small interfering (siRNAs). Most 24-nt siRNAs dependent on RNA Pol IV and RNA-DEPENDENT POLYMERASE 2 (RDR2) establish DNA methylation at thousands of genomic loci a process called RNA-directed (RdDM). In Brassica rapa , RdDM is required the maternal sporophyte for successful seed development. Here, we demonstrate that number siRNA account over 90% expression during B. These exhibit unique characteristics...
Abstract Twenty-four-nucleotide (nt) small interfering RNAs (siRNAs) maintain asymmetric DNA methylation at thousands of euchromatic transposable elements in plant genomes a process called RNA-directed (RdDM). RdDM is dispensable for growth and development Arabidopsis thaliana, but required reproduction other plants, such as Brassica rapa. The 24-nt siRNAs are abundant maternal reproductive tissue, due largely to overwhelming expression from few loci the ovule developing seed coat, termed...
Ablation of cells by the controlled expression a lethal gene can be used to engineer plant traits such as male sterility and disease resistance. However, it may not possible achieve sufficient specificity prevent secondary effects in non-targeted tissues. In this paper we demonstrate that extracellular ribonuclease, barnase, engineered into two complementary fragments, allowing overlapping promoter enhance targeting specificity. Using transient system, first show barnase split inactive...
Abstract Salicylic acid (SA) is a critical mediator of plant innate immunity. It plays an important role in limiting the growth and reproduction virulent powdery mildew (PM) Golovinomyces orontii on Arabidopsis (Arabidopsis thaliana). To investigate this later phase PM interaction played by SA, we performed replicated global expression profiling for wild-type SA biosynthetic mutant isochorismate synthase1 (ics1) from 0 to 7 d after infection. We found that ICS1-impacted genes constitute 3.8%...
The tomato HIGH PIGMENT-2 gene encodes an orthologue of the Arabidopsis nuclear protein DE-ETIOLATED 1 (DET1). From genetic analyses it has been proposed that DET1 is a negative regulator light signal transduction, and recent results indicate may control light-regulated expression at level chromatin remodelling. To gain further understanding about function during plant development, we generated range overexpression constructs introduced them into tomato. Unexpectedly, only observed...
Vertebrates possess three isozyme forms of fructose diphosphate aldolase.We have isolated two overlapping chicken genomic clones which encode the liverspecific form this enzyme; we identified 5' and 3' ends gene by a combination primer extension analysis S1 mapping; determined entire nucleotide sequence including 1400 base pairs (bp) from regions flanking gene.The transcriptional unit for aldolase B spans 8700 bp contains eight intervening sequences, 4600-bp intron in non-coding region.On...
In vertebrates, conserved noncoding elements (CNEs) are functionally constrained sequences that can show striking conservation over >400 million years of evolutionary distance and frequently located megabases away from target developmental genes. Conserved (CNSs) in plants much shorter, it has been difficult to detect among distantly related genomes. this article, we not only CNS be detected throughout the eudicot clade flowering plants, but also a subset 37 CNSs found all (diverging ∼170...
Transposable elements (TEs) are a ubiquitous feature of plant genomes. Because the threat they post to genome integrity, most TEs epigenetically silenced. However, even closely related species often have dramatically different populations TEs, suggesting periodic rounds activity and silencing. Here, we show that process
Abstract We have shown before that the Ac element from maize bz-m2(Ac) allele, located in short arm of chromosome 9 (9S), transposes preferentially to sites are linked bz donor locus. Yet, about half transpositions recovered receptor not In this study, we analyzed distribution those unlinked sites. Thirty-seven transposed (trAc) elements recombined independently locus were mapped using a set wx reciprocal translocations. found for trAs was random. Ten trAcs 9L, i.e., had physically, if...
ABSTRACT 24-nt small interfering siRNAs maintain asymmetric DNA methylation at thousands of euchromatic transposable elements in plant genomes a process call RNA-directed Methylation (RdDM). RdDM is dispensable for growth and development Arabidopsis, but required reproduction other species, such as Brassica rapa. are particularly abundant maternal reproductive tissue, due largely to overwhelming expression from number loci the ovule developing seed coat, termed siren loci. Recently it was...
Abstract Small RNAs are abundant in plant reproductive tissues, especially 24-nt short interfering (si)RNAs. Most siRNAs dependent on RNA Pol IV and RDR2, establish DNA methylation at thousands of genomic loci a process called RNA-directed (RdDM). In Brassica rapa , RdDM is required the maternal sporophyte for successful seed development. Here we demonstrate that small number siRNA account over 90% expression during B. These exhibit unique characteristics with regard to their copy...