Nowadays companies can solve production systems problems by variety of software solutions, but in practice it is known that often difficult to interpret these outputs, illustrate and further integrate. One possibility the use augmented virtual reality for planning systems. This article deals with construction experimental sorting workplace as a model case system. Weak points solution are solved using reality, which helps simulate process real time, this displayed product moving on belt...

Abstract Background The pathophysiology of sepsis-induced acute kidney injury remains elusive. Although mitochondrial dysfunction is often perceived as the main culprit, data from preclinical models yielded conflicting results so far. aim this study was to assess immune-metabolic background sepsis-associated renal using sequential biopsy approach with mitochondria function evaluation in a large clinically relevant porcine mimicking two different paces and severity sepsis couple traditional...

ABSTRACT This article contributes to the literature on banks' strategies for increasing capital requirements in period of 2017–2021. We analysed a sample 85 European banks and distinguished between subgroups according bank size, capitalisation, riskiness. examined their responses higher following issuance finalised Basel III reforms increased regulatory supervisory scrutiny COVID‐19 outbreak. found evidence that adjustments towards ratios were more pronounced faster they depended specific...

dialysis-induced inflammatory response including leukocyte and complement activation is considered a significant cofactor of chronic morbidity in long-term hemodialysis (HD) patients. The aim this study was to provide better insight into its molecular background.in 16 patients, basic biocompatibility markers, i.e. counts C5a levels, were monitored during HD on polysulfone membrane. Proteins adsorbed dialyzers eluted separated by 2-DE. Selected proteins identified MS; ficolin-2 plasma levels...

Mitochondria are considered central regulator of the aging process; however, majority studies dealing with impact age on mitochondrial oxygen consumption focused skeletal muscle concluding (although not uniformly) a general declining trend advancing age. In addition, gender related differences in respiration have been satisfactorily described yet. The aim present study was to evaluate various organs male and female Fischer 344 rats at ages 6, 12 24 months. Mitochondrial homogenized (skeletal...

Additional urinary biomarkers for diabetic nephropathy (DN) are needed, providing early and reliable diagnosis new insights into its mechanisms. Rigorous selection criteria homogeneous study population may improve reproducibility of the proteomic approach. Long-term type 1 diabetes patients without metabolic comorbidities were included, 11 with sustained microalbuminuria (MA) 14 MA (nMA). Morning urine proteins precipitated resolved by 2D electrophoresis. Principal component analysis (PCA)...

Abstract Spinocerebellar ataxia 1 (SCA1) is a devastating neurodegenerative disease associated with cerebellar degeneration and motor deficits. However, many patients also exhibit neuropsychiatric impairments such as depression apathy; nevertheless, the existence of causal link between psychiatric symptoms SCA1 neuropathology remains controversial. This study aimed to explore behavioral deficits in knock-in mouse (SCA1 154Q/2Q ) model identify underlying neuropathology. We found that mice...

Sepsis is a systemic response to infection commonly found in critically ill patients and associated with multi-organ failure high mortality rate. Its pathophysiology molecular mechanisms are complicated remain poorly understood. In the present study, we performed proteomics investigation characterize early host responses sepsis as determined by an altered plasma proteome porcine model of peritonitis-induced sepsis, which simulated several clinical characteristics human syndrome....

The complex pathogenesis of sepsis and septic shock involves myocardial depression, the pathophysiology which, however, remains unclear. In this study, cellular mechanisms depression were addressed in a clinically relevant, large animal (porcine) model shock. Sepsis was induced by fecal peritonitis 8 anesthetized, mechanically ventilated, instrumented pigs both sexes continued for 24 hours. control pigs, an identical experiment but without induction performed. vitro analysis cardiac function...

This study exploited the possibility to detect Citrobacter freundii-derived CMY-2-like cephalosporinases in Enterobacteriaceae clinical isolates using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Periplasmic proteins were prepared a modified sucrose method and analyzed by MALDI-TOF MS. A ca. 39,850-m/z peak, confirmed represent C. freundii-like β-lactamase in-gel tryptic digestion followed MALDI-TOF/TOF MS, was observed only CMY-producing...

Propolis is a natural product that honeybees collect from various plants. It known for its beneficial pharmacological effects. The aim of our study was to evaluate the impact propolis on human sperm motility, mitochondrial respiratory activity, and membrane potential. Semen samples 10 normozoospermic donors were processed according World Health Organization criteria. effects motility activity parameters tested in fresh ejaculate purified spermatozoa. preserved progressive spermatozoa native...

Burden of obesity is increasing in the contemporary world. Although multifactorial origin, appropriate mitochondrial function adipocytes emerges as a factor essential for healthy adipocyte differentiation and adipose tissue function. Our study aimed to evaluate functions human adipose-derived mesenchymal stem cells committed adipogenesis. On days 0, 4, 10, 21 adipogenesis, we have characterized proliferation viability, quantified lipid accumulation maturing cells, performed qualitative...

Members of neuropeptide B/W signaling system have been predominantly detected and mapped within the CNS. In rat, this includes B (NPB), W (NPW) their specific receptor NPBWR1. This has a wide spectrum functions including role in modulation inflammatory pain neuroendocrine functions. Expression NPB, NPW NPBWR1 separate heart compartments, dorsal root ganglia (DRG) stellate was proven by RT-qPCR, Western blot (WB) immunofluorescence. Presence mRNA for all tested genes compartments ganglia. The...

Although the burden of septic acute kidney injury continues to increase, molecular pathogenesis remains largely obscure. The aim this exploratory study was a discovery-driven analysis dynamic tissue protein expression changes applied for first time in classic large mammal model sepsis. To achieve goal, analyses alterations were performed serial samples cortical biopsies (before, 12 and 22 h sepsis) mechanically ventilated pigs challenged with continuous infusion pseudomonas aeruginosa...

Hyperbaric oxygen (HBO) therapy, i.e. breathing pure under increased environmental pressures serves as a treatment for diverse medical conditions. However, elevated concentration can be detrimental to central nervous system or lungs. Our study aimed evaluate the effects of repeated exposure HBO on mitochondrial respiration assessed by high-resolution respirometry (HRR), cell viability estimated PrestoBlue® reaction, morphology analyzed routine phase contrast and fluorescent microscopy,...

Fractionated Plasma Separation, Adsorption and Dialysis (Prometheus) has a well-documented capacity to remove protein-bound organic toxins in patients with liver failure. However, the compositions of adsorbed proteins remain unknown. Elution both adsorbers constituting Prometheus system was performed following 6-h session patient acute on chronic Sodium dodecylsulphate employed elute from neutral adsorber (P1), while acetic acid applied cationic one (P2). Eluted were resolved by...

In order to successfully acquire knowledge in area of machinery safety for engineering students it is necessary adopt elementary principles associated with risk assessment. Identification possible hazards an important part assessment and need take also hands-on training supplement their learning process. It clear that one major obstacle improve the problem allowing learners work directly hazardous equipment. Traditional approach based on use slide show presentations enhanced by animations or...

Propofol is a short-acting hypnotic agent used in human medicine for sedation and general anesthesia. Its administration can be associated with serious cardiovascular side-effects that include decrease arterial blood pressure cardiac output. The aim of the present study was to evaluate propofol effects on mitochondrial respiration, myocardial contractility electrophysiology same samples isolated from heart ventricles adult rats. Mitochondrial oxygen consumption measured permeabilized...

The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis biofilm adsorbed dialysis membrane. Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different regimens, namely citrate, heparin, anticoagulation-free (control). Following regular session (4 hours, polysulfone membrane), dialyzers flushed the surface eluted by acetic acid. Protein composition...