A5058919343- Plant Molecular Biology Research
- Plant Reproductive Biology
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Plant Physiology and Cultivation Studies
- RNA Research and Splicing
- RNA modifications and cancer
- Flowering Plant Growth and Cultivation
- RNA regulation and disease
- Cellular transport and secretion
- Circadian rhythm and melatonin
- Endoplasmic Reticulum Stress and Disease
- ATP Synthase and ATPases Research
University of North Carolina at Chapel Hill
2012-2022
Max Planck Society
2002-2007
John Innes Centre
2000-2002
Max Planck Institute for Plant Breeding Research
2002
Summary CONSTANS promotes flowering of Arabidopsis in response to long‐day conditions. We show that is a member an gene family comprises 16 other members. The CO‐Like proteins encoded by these genes contain two segments homology: zinc finger containing region near their amino terminus and CCT (CO, CO‐Like, TOC1) domain carboxy terminus. Analysis seven classical co mutant alleles demonstrated the mutations all occur within either or domain, confirming regions homology are important for CO...
Auxin regulates plant growth and development in part by activating gene expression. Arabidopsis thaliana SMALL AUXIN UP RNAs (SAURs) are a family of early auxin-responsive genes with unknown functionality. Here, we show that transgenic lines expressing artificial microRNA constructs (aMIR-SAUR-A or -B) target SAUR subfamily (SAUR61-SAUR68 SAUR75) had slightly reduced hypocotyl stamen filament elongation. In contrast, plants SAUR63:GFP SAUR63:GUS fusions long hypocotyls, petals filaments,...
For self-pollinating plants to reproduce, male and female organ development must be coordinated as flowers mature. The Arabidopsis transcription factors AUXIN RESPONSE FACTOR 6 (ARF6) ARF8 regulate this complex process by promoting petal expansion, stamen filament elongation, anther dehiscence, gynoecium maturation, thereby ensuring that pollen released from the anthers is deposited on stigma of a receptive gynoecium. ARF6 induce jasmonate production, which in turn triggers expression MYB21...
The hormone auxin regulates growth largely by affecting gene expression. By studying Arabidopsis (Arabidopsis thaliana) mutants deficient in AUXIN RESPONSE FACTORS (ARFs), we have identified three ARF proteins that are required for auxin-responsive hypocotyl elongation. Plants these factors reduced responses to environmental conditions increase levels, including far-red-enriched light and high temperature. Despite having decreased responses, the ARF-deficient plants responded brassinosteroid...
Three genetic pathways promote flowering of Arabidopsis under long photoperiods. These are represented by the genesCO, FCA, and GA1, which act in long-day, autonomous, gibberellin pathways, respectively. To test whether these only that photoperiods, co-2 fca-1 ga1-3triple mutant was constructed. plants never flowered long- or short-day conditions, indicating three impaired mutations absolutely required for conditions. The triple background represents a "vegetative ground state" enabling...
The plant shoot is derived from the apical meristem, a group of stem cells formed during embryogenesis. Lateral organs form on an adult primordia that arise flanks meristem. Environmental stimuli such as light, temperature and nutrient availability often influence shape identity develop these primordia. In particular, transition forming vegetative lateral to producing flowers occurs in response environmental cues. This requires increased expression genes confer floral identity, Arabidopsis...
In plants, regulated cell expansion determines organ size and shape. Several members of the family redundantly acting Small Auxin Up RNA (SAUR) proteins can stimulate plasma membrane (PM) H+-ATPase proton pumping activity by inhibiting PM-associated PP2C.D phosphatases, thereby increasing PM electrochemical potential, acidifying apoplast, stimulating expansion. Similarly, Arabidopsis thaliana SAUR63 was able to increase growth various organs, antagonize PP2C.D5 phosphatase, activity. Using a...
In Arabidopsis, two myb-related proteins, LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), play key roles in the control of circadian rhythms. Photoperiodic flowering elongation hypocotyls are regulated by a clock. Here, we report isolation characterization novel deletion allele (lhy-14) gain-of-function (lhy-2) lhy. Based on long hypocotyl late phenotypes under days, lhy-2 was isolated as an intragenic suppressor lhy-12, one loss-of-function alleles Although lhy-12...