A5005230317- Plant Molecular Biology Research
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Greenhouse Technology and Climate Control
- Plant nutrient uptake and metabolism
- Health, Environment, Cognitive Aging
- Plant Water Relations and Carbon Dynamics
- Retinal Development and Disorders
- Genetics, Bioinformatics, and Biomedical Research
- Viral Infectious Diseases and Gene Expression in Insects
- Neurobiology and Insect Physiology Research
- RNA regulation and disease
- Plant Pathogenic Bacteria Studies
- Plant responses to water stress
- Retinoids in leukemia and cellular processes
- Plant Reproductive Biology
- Mass Spectrometry Techniques and Applications
- Plant Virus Research Studies
- Pancreatic function and diabetes
- Patient Safety and Medication Errors
- Plant Gene Expression Analysis
- Microbial Inactivation Methods
- Simulation-Based Education in Healthcare
- Polar Research and Ecology
Wageningen University & Research
2020-2023
Centro Nacional de Biotecnología
2018-2020
Trident Technical College
2020
Utrecht University
2016-2019
Consejo Superior de Investigaciones Científicas
2019
Institut des Sciences des Plantes de Montpellier
2018
Canadian Food Inspection Agency
2003-2017
Agriculture and Agri-Food Canada
2017
Children's Hospital of Philadelphia
2016
University of Bristol
2012-2016
Plants in dense vegetation compete for resources and detect competitors through reflection of far-red (FR) light from surrounding plants. This causes a reduced red (R):FR ratio, which is sensed phytochromes. Low R:FR induces shade avoidance responses the shoot also changes root system architecture, although this has received little attention so far. Here, we investigate molecular mechanisms detection regulates development Arabidopsis thaliana We do using combination microscopy, gene...
We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these include the need better understand how plants detect water availability, temperature, salinity, rising carbon dioxide (CO2) levels; environmental signals interface endogenous signaling development (e.g. circadian clock flowering time); this integrated controls downstream responses stomatal regulation,...
Significance UV-B is a key component of sunlight. In plants, perceived by the photoreceptor protein UVR8. Along with regulating photoprotective responses, dramatically inhibits stem elongation. Here we show how regulates plant architecture, using agriculturally important shade avoidance response. When grown in dense stands, plants use reflected far-red light signals from neighbors to detect threat shading. many species, these drive rapid elongation responses overtop competitors. UVR8...
Abstract In plants, light represents an important environmental signal that triggers the production of photosynthetically active chloroplasts. This developmental switch is critical for plant survival because chlorophyll precursors accumulate in darkness can be extremely destructive when illuminated. Thus, plants have evolved mechanisms to adaptively control plastid development during transition into light. Here, we report gibberellin (GA)-regulated DELLA proteins play a crucial role...
Small increases in ambient temperature can elicit striking effects on plant architecture, collectively termed thermomorphogenesis [1Quint M. Delker C. Franklin K.A. Wigge P.A. Halliday K.J. van Zanten Molecular and genetic control of thermomorphogenesis.Nat. Plants. 2016; 2: 15190Crossref PubMed Scopus (304) Google Scholar]. In Arabidopsis thaliana, these include marked stem elongation leaf elevation, responses that have been predicted to enhance cooling [2Gray W.M. Östin A. Sandberg G....
Conventional detection of viruses and virus-like diseases plants is accomplished using a combination molecular, serological, biological indexing. These are the primary tools used by plant virologists to monitor ensure trees free known viral pathogens. The indexing assay, or bioassay, considered be “gold standard” as it only method three that can detect new, uncharacterized, poorly characterized disease agents. Unfortunately, this also most labor intensive take up years complete. Next...
The hormone auxin regulates growth largely by affecting gene expression. By studying Arabidopsis (Arabidopsis thaliana) mutants deficient in AUXIN RESPONSE FACTORS (ARFs), we have identified three ARF proteins that are required for auxin-responsive hypocotyl elongation. Plants these factors reduced responses to environmental conditions increase levels, including far-red-enriched light and high temperature. Despite having decreased responses, the ARF-deficient plants responded brassinosteroid...
Abstract Alterations in light quality significantly affect plant growth and development. In canopy shade, phytochrome photoreceptors perceive reduced ratios of red to far-red (R:FR) initiate stem elongation enable plants overtop competitors. This shade avoidance response is achieved via the stabilisation activation PHYTOCHROME INTERACTING FACTORs (PIFs) which elevate auxin biosynthesis. UV-B inhibits by reducing abundance activity PIFs, yet molecular mechanisms controlling PIF are unknown....
Summary Plants detect the presence of neighbouring vegetation by monitoring changes in ratio red ( R ) to far‐red FR wavelengths : ambient light. Reductions are perceived phytochrome family plant photoreceptors and initiate a suite developmental responses termed shade avoidance syndrome. These include increased elongation growth stems petioles, enabling plants overtop competing vegetation. The majority experiments performed at standard laboratory growing temperatures (>20°C). In these...
Standardized sample preparation to reduce proteome complexity facilitates subsequent analysis. Here we describe a robust sequential extraction method that enables simple fractionation of proteins in their native state according subcellular localization, yielding four subproteomes enriched (a) cytosolic; (b) membrane and organelle-localized; (c) soluble DNA-associated nuclear (d) cytoskeletal proteins. Efficiency selectivity is demonstrated by morphological-, two-dimensional electrophoresis...
Abstract Plants have a remarkable capacity to acclimate their environment. Acclimation is enabled large degree by phenotypic plasticity, the extent of which confers selective advantage, especially in natural habitats. Certain key events evolution triggered adaptive bursts necessary cope with drastic environmental changes. One such event was colonization land 400–500 million years ago. Compared most aquatic habitats, fluctuations abiotic parameters became more pronounced, generating...
Objectives To assess the effectiveness of pediatric simulation by remote facilitation. We hypothesized that facilitation is more effective compared to an on-site facilitator. defined as a facilitator remotely (1) introduces simulation-based learning and environment, (2) runs scenarios, (3) performs debriefing with Methods A program for medical students during rotation was implemented. Groups were allocated either or depending on availability telemedicine technology. Both groups had identical...
Plum pox virus (PPV) isolate 3174-01 was detected by triple-antibody sandwich enzyme-linked immunosorbent assay using the universal PPV monoclonal antibody (MAb) 5B as secondary antibody, and reverse-transcription polymerase chain reaction (RT-PCR) primers that amplify a 243-bp fragment in C-terminus of coat protein coding region. The restriction sites RsaI AluI were absent from this fragment, which is feature unique to PPV-C isolates. replaced GTAA/GTGA GGCA, respectively. There 95 99, 94,...
The California Simulation of Evapotranspiration Applied Water (Cal-SIMETAW) model is a new tool developed by the Department Resources and University California, Davis to perform daily soil water balance determine crop evapotranspiration (ETc), applied (ETaw), (AW) for use in resources planning. ETaw seasonal estimate needed irrigate assuming 100% irrigation efficiency. accounts soils, coefficients, rooting depths, seepage, etc. that influence balance. It provides spatial climate information...
Avirulence gene D ( avrD ) is carried on the B‐plasmid of plant pathogen Pseudomonas syringae pv. tomato with plasmid‐borne homologs widely distributed among Pseudomonads. We now report sequences in soft rot Erwinia carotovora that cross‐hybridize to suggesting a conserved function beyond avirulence. Alternatively, may have been transferred horizontally species: (i) DNA linked shows evidence class II transpositions and contains novel IS 3 ‐related insertion sequence, (ii) short are similar...