Polyphenols such as epigallocatechin-3-gallate (EGCG) from green tea extract can exert a growth-suppressive effect on human pancreatic cancer cells in vitro . In pursuit of our investigations to dissect the molecular mechanism EGCG action cancer, we observed that antiproliferative carcinoma is mediated through programmed cell death or apoptosis evident nuclear condensation, caspase-3 activation and poly-ADP ribose polymerase (PARP) cleavage. EGCG-induced accompanied by growth arrest at an...

In murine embryonic fibroblasts, N-acetyl-l-cysteine (NAC), a GSH generating agent, enhances hypoxic apoptosis by blocking the NFκB survival pathway (Qanungo, S., Wang, M., and Nieminen, A. L. (2004) J. Biol. Chem. 279, 50455-50464). Here, we examined sulfhydryl modifications of p65 subunit that are responsible for inactivation. MIA PaCa-2 pancreatic cancer cells, hypoxia increased p65-NFκB DNA binding transactivation 2.6- 2.8-fold, respectively. NAC blocked these events without having an...

Background— Hypertension doubles coronary heart disease (CHD) risk. Treating hypertension only reduces CHD risk ≈25%. hypercholesterolemia in hypertensive patients residual >35%. Methods and Results— To assess progress concurrent control, National Health Nutrition Examination Surveys 1988 to 1994, 1999 2004, 2005 2010 were analyzed. was defined by blood pressure ≥140/≥90 mm Hg, current medication treatment, 2-told status; <140/<90 control. Hypercholesterolemia ATP III criteria based...

Lack of recruitment qualified research participants continues to be a significant bottleneck in clinical trials, often resulting costly time extensions, underpowered results, and some cases early termination. Some the reasons for suboptimal include laborious consent processes access at remote locations. While new electronic consents technologies (eConsent) help overcome challenges related readability management, they do not adequately address access. To this, we have developed an innovative...

In this study, we investigated the role of reduced glutathione (GSH) and nuclear factor-κB (NFκB) in hypoxia-induced apoptosis. Hypoxia caused p53-dependent apoptosis murine embryonic fibroblasts transfected with Ras E1A. N-Acetyl-l-cysteine (NAC) but not other antioxidants, such as vitamin E analog trolox epigallocatechin-3-gallate, enhanced caspase-3 activation NAC also two human cancer cell lines, MIA PaCa-2 pancreatic cells A549 lung carcinoma cells. fibroblasts, all three antioxidants...

Abstract The interaction between HIF‐1α, Mdm2, and p53 proteins during hypoxia has received recent attention. Here, we investigated the consequences of HIF‐1α Mdm2 under hypoxic conditions. Endogenous were co‐immunoprecipitated from lysates HCT116 p53WT −/− cells, suggesting that association these two is a p53‐independent event. cellular protein content was not significantly altered in tumor cells. Overexpression resulted an increase cells increased hypoxia‐induced vascular endothelial...

Cardiomyocyte apoptosis is a well-established contributor to irreversible injury following myocardial infarction (MI). Increased cardiomyocyte associated also with aging in animal models, exacerbated by MI; however, mechanisms for this increased sensitivity oxidative stress are unknown. Protein mixed-disulfide formation glutathione (protein glutathionylation) known change the function of intermediates that regulate apoptosis. Since glutaredoxin (Grx) specifically catalyzes protein...

Apoptosis repressor with caspase recruitment domain is expressed at high levels in brain and myogenic tissues, consistent a role to inhibit apoptosis the terminally differentiated cells. Expression of ARC cancers not known. In this study, we reported that was highly various non‐myogenic non‐neurogenic human rat cancer cell lines. Unexpectedly, localized almost exclusively nuclei cells, which unlike cytoplasmic localization non‐cancer Furthermore, nuclear cells did co‐localize nucleolus...

Clinical and animal studies have documented that hearts of the elderly are more susceptible to ischemia/reperfusion damage compared young adults. Recently we found aging-dependent increase in susceptibility cardiomyocytes apoptosis was attributable decrease cytosolic glutaredoxin 1 (Grx1) concomitant NF-κB-mediated expression anti-apoptotic proteins. Besides primary localization cytosol, Grx1 also exists mitochondrial intermembrane space (IMS). In contrast, Grx2 is confined matrix. Here...

We must include rural participants in health-related research if we are to address disparities and inequity, particularly mental health. However, the first step of process, person, witnessed, signed informed consent is often a limiting factor insurmountable barrier precisely type (e.g., telehealth) designed overcome barriers geographic distance travel time. Telehealth, or provision medical care services patients by means audio/video procedure-specific technology, addresses some health...

Clinical trials are key to ensuring high-quality, effective, and safe health care interventions, but there many barriers their successful timely implementation. Difficulties with participant recruitment enrollment largely affected by difficulties obtaining informed consent. Teleconsent is a telemedicine- based approach consent offers unique solution limitations of traditional approaches.

Microtubule inhibitor-induced Bcl2 phosphorylation is detrimental to its antiapoptotic function. Phosphorylation of predominantly occurs on two serine residues (70 and 87) in cells arrested at G2-M phase by microtubule disarraying agents. Phospho can associate with a cis-trans peptidyl prolyl isomerase, Pini. Pini homologues are known target the proline residue carboxyl terminal phosphorylated threonine or mitotic phosphoproteins, such as Bcl2. However, it was not clear how an extranuclear...

Approximately 20% of children with sickle cell disease (SCD) have microalbuminuria (MA). Very little is known about the progression MA in and young adults SCD.In this study, we analyzed 5-year EMR data 373 [with ≥2 microalbumin-to-creatinine (MA/Cr) ratio measurements] followed at Medical University South Carolina to determine rate, direction, magnitude, predictors MA/Cr change over time.Age range was 1-22 years; mean 10.2 ± 5.2 years, 49.5% were males. Median follow-up duration 3.12 1.16...

In India, the need for rural palliative care is increasing with rising number of people diagnosed late-stage cancers. Rural areas also have a shortage trained medical personnel to deliver care. To address these needs, home-based program using community health workers (CHWs) facilitate delivery was developed extend reach cancer center's services outside Kolkata, India. The research question guiding this qualitative study was, how feasible, useful, and acceptable from perspectives clinical...

40 million people in the world are need of palliative care, but only one-seventh that population receive services. Underuse care low resource countries exacerbates suffering patients with life limiting illnesses such as cancer.The current study was conducted to identify barriers, facilitators and recommended strategies for informing development a home-based intervention poor medically underserved rural Kolkata, India.Semi-structured interviews were 20 clinical patient stakeholders India....

Informed consent is among the biggest challenges in recruiting participants for clinical research studies. Researchers face many conducting trials, some of which include budgetary restrictions, lack trained personnel, and difficulty study participants-particularly minorities from rural communities.The objective this to utilize telemedicine improve informed process trials We aim assess feasibility efficacy teleconsent intervention residents urban settings.This will be conducted separately yet...