A5023419556- Cellular transport and secretion
- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Sphingolipid Metabolism and Signaling
- Photosynthetic Processes and Mechanisms
- Protein Degradation and Inhibitors
- Lysosomal Storage Disorders Research
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Microfluidic and Bio-sensing Technologies
- Genetic Neurodegenerative Diseases
- Toxoplasma gondii Research Studies
- Peptidase Inhibition and Analysis
- Microbial Metabolic Engineering and Bioproduction
- RNA modifications and cancer
- Plant Surface Properties and Treatments
- Neurobiology and Insect Physiology Research
- Protein Tyrosine Phosphatases
- Metabolism, Diabetes, and Cancer
- Transgenic Plants and Applications
- Flavonoids in Medical Research
Nagoya University
2017-2024
Hokkaido University
2008-2018
Bio-Medical Science (South Korea)
2017
Tokyo Institute of Technology
2014
Hokkaido Pharmaceutical University
2011
National Institutes of Natural Sciences
2008
National Institute for Basic Biology
2006-2008
University Medical Center
2003
Morpho (United States)
2003
The University of Tokyo
2001-2002
Atg18 is essential for both autophagy and the regulation of vacuolar morphology. The latter process mediated by phosphatidylinositol 3,5-bisphosphate binding, which dispensable autophagy. also binds to 3-phosphate (PtdIns(3)P) in vitro. Here, we investigate relationship between PtdIns(3)P-binding Using an variant, Atg18(FTTG), unable bind phosphoinositides, found that PtdIns(3)P binding full activity selective nonselective Atg18(FTTG) formed a complex with Atg2 normal manner, Atg18-Atg2...
Abstract Differentiation into a tracheary element (TE) is typical example of programmed cell death (PCD) in the developmental processes vascular plants. In PCD process TE degrades its cellular contents and becomes hollow corpse that serves as water conduct. Using zinnia (Zinnia elegans) culture we obtained serial observations single living cells undergoing by confocal laser scanning microscopy. Vital staining was performed relative fluorescence intensity measured, revealing tonoplast swollen...
Atg5 is covalently modified with a ubiquitin-like modifier, Atg12, and the Atg12-Atg5 conjugate further forms complex multimeric protein Atg16. The Atg12-Atg5.Atg16 plays an essential role in autophagy, bulk degradation system conserved all eukaryotes. We have reported here crystal structure of complexed N-terminal region Atg16 at 1.97A resolution. comprises two domains that flank helix-rich domain. has helical bound to groove formed by these three domains. In vitro analysis showed Arg-35...
In the yeast Saccharomyces cerevisiae, two similar phosphatidylinositol 3-kinase complexes (complexes I and II) function in distinct biological processes, complex autophagy II vacuolar protein sorting via endosomes. Atg14p is only integrated into I, likely facilitating of autophagy. Deletion analysis revealed that N-terminal region containing coiled-coil structures was essential sufficient for localized to pre-autophagosomal structure (PAS) membranes, whereas Vps38p, a component specific II,...
Autophagy is a process by which cells recycle cytoplasm and defective organelles during stress situations such as nutrient starvation. It can also be used host an immune defense mechanism to eliminate infectious pathogens. Here we describe the use of autophagy survival virulence-associated trait human fungal pathogen Cryptococcus neoformans. We report that mutant form C. neoformans lacking Vps34 PI3K (vps34Delta), known involved in ascomycete yeast, was formation autophagy-related 8-labeled...
Vps34, the sole PtdIns 3‐kinase in yeast, is essential for autophagy. Here, we show that lipid‐kinase activity of Vps34 required autophagy, implying an role its product PtdIns(3) P . The protein‐kinase Vps15, a regulatory subunit complex, also efficient We monitored distribution living cells using specific indicator, 2xFYVE domain derived from mammalian Hrs. was abundant at endosomes and on vacuolar membrane during logarithmic growth phase. Under starvation conditions, observed massive...
Eukaryotic cells can massively transport their own cytoplasmic contents into a lytic compartment, the vacuole/lysosome, for recycling through conserved system called autophagy. The key process in autophagy is sequestration of within double-membrane structure, autophagosome. Autophagosome formation requires elaborate cooperation Atg ( u t opha g y-related) proteins and lipid molecules. Phosphorylation phosphatidylinositol (PtdIns) by PtdIns 3-kinase, Vps34, step coordinating Vps34 forms two...
Phosphorylation of phosphatidylinositol (PtdIns) by a PtdIns 3-kinase is an essential process in autophagy. Atg14, specific subunit one the complexes, targets complex to probable site autophagosome formation, thereby, sorting function specifically The N-terminal half containing coiled-coil domains, required form and target it proper site. C-terminal yeast Atg14 suggested be involved formation normal-sized autophagosome. mammalian contains Barkor/Atg14(L) autophagosome-targeting sequence...
Protein analysis strategies involving targeted protein degradation are powerful approaches to determine gene functions. Auxin-inducible degron (AID) is among the most widely used methods for target knockdown. This system enables rapid depletion of AID-tagged proteins in an auxin-dependent manner. Various improved AID have been developed date; however, requirement tag remains a common challenge. Here, we demonstrated efficiency affinity-linker-based super-sensitive condition-knockdown...
Monodehydroascorbate reductase (MDAR) isoforms exist in mitochondria, chloroplasts, cytosol and microbodies. Two putative MDAR sequences with an extended N-terminal region are found Arabidopsis. They differ the length of extension by 21 bp. We have shown that these two arise from a single gene use multiple transcription starts. Green fluorescent protein was fused to each extension, revealing longer shorter fusion proteins were imported into mitochondria respectively. These results...
Phosphorylation of phosphatidylinositol (PtdIns) by PtdIns 3-kinase is essential for autophagy. However, the distribution and function enzymatic product, 3-phosphate (PtdIns(3)P), has been unknown. We monitored PtdIns(3)P during autophagy live imaging, biochemistry, electron microscopy, found that massively delivered into vacuole via highly enriched as a membrane component elongating isolation membranes autophagosome rather than an enclosed cargo, implying direct involvement in formation....
In yeast, external alkalization and alteration in plasma membrane lipid asymmetry are sensed by the Rim101 pathway. It is currently under debate whether signal elicited transduced to downstream molecules at or via endocytosis of Rim21 sensor protein late endosome. We found that molecules, including arrestin-related Rim8, calpain-like Rim13, scaffold Rim20, accumulated upon accumulation was dependent on Rim21. Snf7, an endosomal sorting complex required for transport (ESCRT) III subunit also...
Proper inheritance of functional organelles is vital to cell survival. In the budding yeast, Saccharomyces cerevisiae, endoplasmic reticulum (ER) stress surveillance (ERSU) pathway ensures that daughter cells inherit a ER. Here, we show ERSU activated by phytosphingosine (PHS), an early biosynthetic sphingolipid. Multiple lines evidence support this: (1) Reducing PHS levels with myriocin diminishes ability induce phenotypes. (2) Aureobasidin A treatment, which blocks conversion intermediates...
Two species of rice have been independently domesticated from different ancestral wild in Asia and Africa. Comparison mutations that underlie phenotypic physiological alterations associated with domestication traits these gives insights into the history both regions. Asian cultivated rice, Oryza sativa, African glaberrima , modified improved for common beneficial humans, including erect plant architecture, nonshattering seeds, nonpigmented pericarp, lack awns. Independent orthologous genes...
Very long-chain fatty acids (VLCFAs), with chain-length greater than 20 carbons, possess a wide range of biological functions. However, their roles at the molecular level remain largely unknown. In present study, we screened for multicopy suppressors that rescued temperature-sensitive growth VLCFA-limited yeast cells, and identified VPS21 gene, encoding Rab GTPase, as such suppressor. When vps21Δ mutation was introduced into deletion mutant SUR4 which encodes VLCFA elongase, synthetic defect...
Changes in membrane dynamics are known to occur cells faced with starvation. However, the functions of major lipid components biological membranes, sphingolipids, during starvation response remain unclear. In this study, we found that yeast lacking genes encoding mannosylinositol phosphorylceramide ( MIPC ) synthases csg1 Δ csh1 Δ) underwent rapid cell death upon nitrogen starvation, but not carbon or and Addition N a 3 prevented starvation–induced cells, indicating energy production is...
Yeast cells sense alterations in the plasma membrane (PM) lipid asymmetry and external alkalization by sensor protein Rim21, which functions Rim101 pathway. signaling is initiated at PM recruitment of complex. The physically associates with cortical endoplasmic reticulum (ER) to form ER-PM contact sites, where several events, exchange, ion transport take place. In present study, we investigated spatial relationship between sites signaling. mostly proceeds outside did not require intact for...
Abstract In yeast, mitochondria are passed on to daughter cells via the actin cable, motor protein Myo2, and adaptor Mmr1. They released from actin-myosin machinery after reaching cells. We report that Mmr1 is rapidly degraded by ubiquitin-proteasome system in Saccharomyces cerevisiae . Redundant ubiquitin ligases Dma1 Dma2 responsible for ubiquitination. Dma1/2-mediated ubiquitination requires phosphorylation, most likely at S414 residue Ste20 Cla4. These kinases mostly localized growing...
Eph receptor tyrosine kinases and their ephrin ligands are overexpressed in various human cancers, including colorectal malignancies, suggesting important roles many aspects of cancer development progression as well cellular repulsive responses. The ectodomain EphB2 is cleaved by metalloproteinases (MMPs) MMP-2/MMP-9 released into the extracellular space after stimulation its ligand. remaining membrane-associated fragment further presenilin-dependent γ-secretase releases an intracellular...