A5049766839- Renal Transplantation Outcomes and Treatments
- Pancreatic function and diabetes
- Xenotransplantation and immune response
- Renal and Vascular Pathologies
- T-cell and B-cell Immunology
- Organ and Tissue Transplantation Research
- Organ Transplantation Techniques and Outcomes
- Tissue Engineering and Regenerative Medicine
- Cytomegalovirus and herpesvirus research
- Diabetes and associated disorders
- Renal and related cancers
- Organ Donation and Transplantation
- Immune Cell Function and Interaction
- Congenital Heart Disease Studies
- Complement system in diseases
- Urological Disorders and Treatments
- Neurological Complications and Syndromes
- interferon and immune responses
- Pharmacogenetics and Drug Metabolism
- Pharmacological Effects and Toxicity Studies
- Genetic Syndromes and Imprinting
- Virus-based gene therapy research
- Animal Genetics and Reproduction
- Ureteral procedures and complications
- Biochemical and Molecular Research
Duke University
2015-2025
Duke Medical Center
2021-2025
Durham Technical Community College
2023
Emory University
2013
University of Toronto
2009
Hospital for Sick Children
2009
SickKids Foundation
2009
Beijing Hua Xin Hospital
2004-2005
Beijing Chao-Yang Hospital
2004
Changsha Medical University
2000
Genetic modification of porcine donors, combined with optimized immunosuppression, has been shown to improve outcomes experimental xenotransplant. However, little is known about in sensitized recipients, a population that could potentially benefit the most from clinical implementation xenotransplantation. Here, five highly allosensitized rhesus macaques received kidney
Abstract Objectives The clinical outcome of prenatally diagnosed congenital heart defects (CHD) continues to be affected significantly by associated extracardiac and chromosomal abnormalities. We sought to: determine the frequency type major abnormalities (with impact on quality life) with fetal CHD; compare detected postnatal autopsy findings in fetuses, find incidence missed prenatal ultrasound. Methods reviewed computerized database Division Cardiology Hospital for Sick Children Toronto...
Prior studies of anti-CD40 ligand (CD40L)–based immunosuppression demonstrated effective prevention islet and kidney allograft rejection in nonhuman primate models; however, clinical development was halted because thromboembolic complications. An anti-CD40L-specific monoclonal antibody, AT-1501 (Tegoprubart), engineered to minimize risk complications by reducing binding Fcγ receptors expressed on platelets while preserving CD40L. tested both a cynomolgus macaque model intrahepatic...
Abstract Sensitized kidney transplant recipients experience high rates of antibody-mediated rejection due to the presence donor-specific antibodies and immunologic memory. Here we show that transient peri-transplant treatment with central complement component C3 inhibitor Cp40 significantly prolongs median allograft survival in a sensitized nonhuman primate model. Despite antibody levels remaining high, fifty percent Cp40-treated primates maintain normal function beyond last day treatment....
Current desensitization and maintenance immunosuppression regimens for kidney transplantation in sensitized individuals show limited ability to control the posttransplant humoral response, resulting high rates of antibody-mediated rejection (ABMR) graft failure. Here, we showed that anti-CD154 monoclonal antibody (mAb)–based more effectively controlled allograft rebound a highly nonhuman primate model compared with tacrolimus-based standard-of-care (SOC) immunosuppression. Desensitization an...
Costimulation blockade with the fusion protein belatacept provides a desirable side effect profile and improvement in renal function compared calcineurin inhibition transplantation. This comes at cost of increased rates early acute rejection. Blockade integrin molecule leukocyte function-associated antigen 1 (LFA-1) has been shown to be an effective adjuvant costimulation rigorous nonhuman primate (NHP) model islet transplantation; therefore, we sought test this combination NHP transplant...
Abstract Background Thrombosis is a known consequence of intraportal islet transplantation, particularly for xenogeneic islets. To define the origins thrombosis after xenotransplantation and relate it to early inflammation, we examined porcine islets transplanted into non‐human primates using dual‐transplant model directly compare characteristics. Methods α1,3‐Galactosyltransferase gene‐knockout (GTKO) with without expression human complement regulatory transgene CD46 (hCD46) were studied....
Costimulation blockade (CoB) via belatacept is a lower-morbidity alternative to calcineurin inhibitor (CNI)-based immunosuppression. However, it has higher rates of early acute rejection. These rejections are mediated in part by memory T cells, which have reduced dependence on the pathway targeted and increased adhesion molecule expression. One such leukocyte function antigen (LFA)-1. LFA-1 exists two forms: commonly expressed, low-affinity form transient, high-affinity form, expressed only...
Porcine islet xenotransplantation is a viable strategy to treat diabetes. Its translation has been limited by the pre-clinical development of clinically available immunosuppressive regimen. We tested two relevant induction agents in non-human primate (NHP) model compare depletional versus nondepletional immunosuppression. Neonatal porcine islets were isolated from GKO or hCD46/GKO transgenic piglets and transplanted via portal vein infusion diabetic rhesus macaques. Induction therapy...
Preexisting donor-specific antibodies (DSA) to MHC antigens increase the risk of antibody-mediated rejection (AMR) in sensitized transplant recipients and reduces graft survival. Pretransplant desensitization with costimulation blockade proteasome inhibition has facilitated transplantation our preclinical nonhuman primate (NHP) model. However, long-term survival is limited by rebound DSA after transplantation. In this study, we performed kidney transplants between highly sensitized,...
P1159 Aims: The calcineurin inhibitors cyclosporine and tacrolimus are the major immunosuppressive agents, It becomes complicated to use them for their interindividual differences in oral bioavailability. CYP3A is enzyme responsible metabolism, CYP3A5 genes have multiple single nucleotide polymorphisms. Our objective was determine relationship between role of genetic polymorphisms with respect variability pharmacokinetics, analyse gene frequency Han people North China. Methods: Kidney...
A51 Aims: To evaluate the effect of a new management transplant ureter and renal transplantation followed by enterocystoplasty in patients with small, dysfunctionalized bladder due to urinary tuberculosis. Methods: The two were 47 42 years old, etiologies dysfunction contraction secondary Three after prior transplantation, 47-year old patient got an aneurysm on transplanted artery function was normal. is filled aseptic saline until discomfort or leakage occurs, instilled volume increased...
A43 Aims: Renal transplantation has become established treatment as the curative therapy for end-stage renal failure. The critical shortage of organs forced use live donor kidneys. number living kidney increased in China recently years. We review our experience with stone disease. Methods: study involved 3 cases Among 52 related transplantations performed at centre between 1998.2 and 2004.2. There were one women two men mean age 35.all them are diagnosised preoperative examination...
A53 Aims: To evaluate the efficacy of extracorporeal nephron-sparing surgery (Bench Surgery) in 2 renal cases who underwent autotransplants. Methods: Angiomyolipoma were diagnosed on both kidneys a 21-year-old male and 39-year-old female patients with hematuria by CT ultrasonography. The examinations indicated that angiomyolipoma was larger than 15cm left side less 5cm right side. A urography inferior function kidney angiomyolipoma. Bench autotransplantation performed due to large tumor size...
A50 Aims: To evaluate the changes of PRA and effect sequential renal transplantation from same cadaver donor in three patients who were highly sensitized to human leukocyte antigens experienced hyperacute rejection during operation. Methods: Three with high panel reactive antibody (PRA, range 87% 100%) treated plasma exchange. After exchange, did not reduced less than 70%. They on hemodialysis had waited for more 2 years. volunteered join our study due economy reasons. receiving cyclosporine...
P167* Aims: This retrospective study describes the surgical techniques and outcomes of living related renal transplantation allografts with multiple arteries. Methods: Between 1984 2001, 380 consecutive transplants were done, including 324 single(group1) 56 (group2) Intracorporeal in situ anastomotic used for 32 grafts arteries, while ex vivo 24. During surgery each one arteries was anastomosed separately to an individual artery. In 2 or more joined together on bench form a common stem,...