A5071050957- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Immune cells in cancer
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Health and Well-being Studies
- Cancer-related gene regulation
- Gastrointestinal motility and disorders
- Polyamine Metabolism and Applications
- Cancer-related molecular mechanisms research
- Reproductive System and Pregnancy
- Liver physiology and pathology
- Fibroblast Growth Factor Research
- Phagocytosis and Immune Regulation
- Industrial Engineering and Technologies
- Neuropeptides and Animal Physiology
- Animal health and immunology
- Material Science and Thermodynamics
- Bioinformatics and Genomic Networks
- Platelet Disorders and Treatments
- Receptor Mechanisms and Signaling
- Gene expression and cancer classification
- Respiratory viral infections research
- Immunotherapy and Immune Responses
- Electric Power Systems and Control
Roslin Institute
2016-2024
University of Edinburgh
2016-2023
University of Florida
2004-2007
The F4/80 antigen, encoded by the Adgre1 locus, has been widely-used as a monocyte-macrophage marker in mice, but its value macrophage other species is unclear, and even questioned. ADGRE1 seven transmembrane G protein-coupled receptor with an extracellular domain containing repeated Epidermal Growth Factor (EGF)-like calcium binding domains. Using new monoclonal antibody, we demonstrated that myeloid differentiation pigs, absent from progenitors bone marrow, highly-expressed mature...
Macrophage colony-stimulating factor (CSF1) is an essential growth and differentiation for cells of the macrophage lineage. To explore role CSF1 in steady-state control monocyte production tissue repair, we previously developed a bioactive protein with longer half-life circulation by fusing pig Fc region IgG1a. CSF1-Fc administration to pigs expanded progenitor pools marrow selectively increased numbers their expression maturation marker CD163. There was rapid increase size liver, extensive...
The need for repeated administration of vaccines is costly, logistically challenging and leads to reduced compliance. Osmosis-driven swelling rupture has been proposed as a mechanism delayed burst release mimic follow-up (booster) injections without requiring further intervention. use microparticles does not guarantee single bolus release, previous studies using monolithic implants failed demonstrate high instant at the moment burst. In this study we developed elastic capsules based on...
Abstract Activated mouse macrophages metabolize arginine via NO synthase (NOS2) to produce as an antimicrobial effector. Published gene expression datasets provide little support for the activation of this pathway in human macrophages. Generation requires coordinated regulation multiple genes. We have generated RNA-sequencing data from bone marrow–derived representative rodent (rat), monogastric (pig and horse), ruminant (sheep, goat, cattle, water buffalo) species, analyzed genes involved...
The domestic pig (Sus scrofa) is both an economically-important livestock species and a model for biomedical research. Two highly-contiguous reference genomes have recently been released. To support functional annotation of the comparative analysis with large human transcriptomic datasets, we aimed to create gene expression atlas. achieve this objective, extended previous approach developed chicken. We down-loaded RNAseq datasets from public repositories, down-sampled common depth quantified...
Abstract Bovine alveolar macrophages (AMs) defend the lungs against pathogens such as Mycobacterium bovis ( M. bovis) , causative agent of bovine tuberculosis. However, little is known about surface molecules expressed by AMs and whether there heterogeneity within population. The purpose this study was to characterise AM cell phenotype using flow cytometry. Bronchoalveolar lavage samples from four different calves were stained with a combination antibodies immune prior cytometric analysis....
Macrophage colony-stimulating factor (CSF1) controls the proliferation and differentiation of cells mononuclear phagocyte system through binding to receptor CSF1R. The expression function CSF1 has been well-studied in rodents humans, but knowledge is lacking other veterinary species. development a novel mouse anti-porcine monoclonal antibody (mAb) facilitates characterisation this growth pigs. Cell surface was confirmed on differentiated macrophage populations derived from blood bone marrow...
Searchable abstracts of presentations at key conferences on reproductive biology and medicine ISSN 2052-1472 (online)
Bovine natural killer (bNK) cells are heterogeneous cell populations defined by constitutive expression of the cytotoxicity receptor, NKp46 (CD335). Two major subsets bNK cells, classified differential CD2, display divergent functions in innate immunity, and hypothesised to contribute adaptive immunity following vaccination. Here we characterised phenotypic variation within afferent lymph node (LN) tissues between CD2+ CD2- subsets, report changes induced BCG which dominate LN, displayed...
s of Platform & Poster Presentations CSM 2008 • Nashville, TN: POSTER PRESENTATIONS: PDF Only
s of Platform & Poster Presentations CSM 2008 • Nashville, TN: POSTER PRESENTATIONS: PDF Only