A5101427926- Animal Nutrition and Physiology
- Immune Response and Inflammation
- Coccidia and coccidiosis research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Veterinary medicine and infectious diseases
- Antimicrobial Peptides and Activities
- Escherichia coli research studies
- Helminth infection and control
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Rabbits: Nutrition, Reproduction, Health
- RNA Interference and Gene Delivery
- Risk and Safety Analysis
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Animal Virus Infections Studies
- Occupational Health and Safety Research
- Phagocytosis and Immune Regulation
- Invertebrate Immune Response Mechanisms
- Galectins and Cancer Biology
- Chemokine receptors and signaling
- Salmonella and Campylobacter epidemiology
Roslin Institute
2015-2025
University of Edinburgh
2011-2025
Beihang University
2023
Tianjin Economic-Technological Development Area
2022
Beijing Agricultural Machinery Research Institute
2021
Biotechnology and Biological Sciences Research Council
2016
The Pirbright Institute
2008-2014
Département Santé Animale
2011
Northern Jiangsu People's Hospital
1998
Dendritic cells (DCs) are bone marrow-derived professional antigen-presenting cells. The in vitro generation of DCs from either marrow or blood is routine mammals. Their distinct morphology and phenotype their unique ability to stimulate naïve T used define DCs. In this study, chicken were cultured the presence recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) interleukin-4 (IL-4) for 7 days. population showed typical DCs, with surface major histocompatibility complex...
In mammals, the inducible cytokine interleukin 10 is a feedback negative regulator of inflammation. To determine extent to which this function conserved in birds, recombinant chicken IL-10 was expressed as secreted human Ig Fc fusion protein (chIL-10-Fc) and used immunise mice. Five monoclonal antibodies (mAb) specifically recognise were generated characterised. Two capture ELISA assays developed detected native chIL-10 from bone marrow-derived macrophages (chBMMs) stimulated with...
Effective mucosal immunity in the intestine involves a fine balance between tolerance of microbiome, recognition and elimination pathogens, inflammatory tissue injury. The anti-inflammatory cytokine IL10 regulates these processes intestines mice humans; activity is also conserved birds. To determine function avian immunity, we generated germ line modifications chicken locus to abolish or reduce expression. In vitro analysis macrophage response lipopolysaccharide confirmed loss protein...
Avian pathogenic Escherichia coli (APEC) infections are a serious impediment to sustainable poultry production worldwide. Licensed vaccines available, but the immunological basis of protection is ill-defined and need exists extend cross-serotype efficacy. Here, we analysed innate adaptive responses induced by commercial in turkeys. Both live-attenuated APEC O78 ΔaroA vaccine (Poulvac® E. coli) formalin-inactivated bacterin conferred significant against homologous intra-airsac challenge model...
Coccidiosis is a major contributor to losses in poultry production. With emerging constraints on the use of in-feed prophylactic anticoccidial drugs and relatively high costs effective vaccines, there are commercial incentives breed chickens with greater resistance this important production disease. To identify phenotypic biomarkers that associated impacts coccidiosis, assess their covariance heritability, 942 Cobb500 broilers were subjected defined challenge Eimeria tenella (Houghton)....
The phosphatidylserine receptor TIM4, encoded by TIMD4, mediates the phagocytic uptake of apoptotic cells. We applied anti-chicken TIM4 mAbs in combination with CSF1R reporter transgenes to dissect function chick (Gallus gallus). During development ovo, was present on large majority macrophages, but expression became more heterogeneous posthatch. Blood monocytes expressed KUL01, class II MHC, and CSF1R-mApple uniformly. Around 50% were positive for surface TIM4. They also many other...
Eimeria maxima is a common cause of coccidiosis in chickens, disease that has huge economic impact on poultry production. Knowledge immunity to E. and the specific mechanisms contribute differing levels resistance observed between chicken breeds congenic lines derived from single breed chickens required. This study aimed define differences kinetics immune response two inbred White Leghorn exhibit differential (line C.B12) or susceptibility 15I) infection by . Line C.B12 15I were infected...
<title>Abstract</title> Research on chemokines in cattle is hampered by the relative lack of reagents. Bovine IP-10 an important inflammatory chemokine and a promising diagnostic biomarker for economically disease (bovine tuberculosis) caused infection with <italic>Mycobacterium bovis</italic>. Currently no monoclonal antibodies are available bovine IP-10. The goal this study was to generate novel mAbs detection using recombinant hybridoma technology. Five were developed cross clone...
Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharide from Gram-negative bacteria, plays a major role in resistance of mice and humans to Salmonella infection. In chickens, may establish carrier state whereby bacteria are able persist the host organism without triggering clinical signs. Based on cellular morphological parameters, we developed method, using antibodies, separate three cecal cell subpopulations: lymphocytes, enterocytes, population encompassing multiple types. We...
Many genes important in immunity are found as multigene families. The butyrophilin members of the B7 family, playing diverse roles co-regulation and perhaps antigen presentation. In humans, a fixed number around major histocompatibility complex (MHC), show striking association with particular autoimmune diseases. chickens, BG encode homologues somewhat different domain organisation. Only few have been characterised, one involved actin-myosin interaction intestinal brush border, another...
Macrophage colony-stimulating factor (CSF1) is an essential growth to control the proliferation, differentiation and survival of cells macrophage lineage in vertebrates. We have previously produced a recombinant chicken CSF1-Fc fusion protein administrated it birds which substantial expansion tissue populations. To further study biology CSF1 chicken, here we generated anti-chicken antibodies (ROS-AV181 183) using as immunogen. The specific binding each monoclonal antibody was confirmed by...
Conventional dendritic cells (cDCs) are antigen-presenting (APCs) that play a central role in linking innate and adaptive immunity. cDCs have been well described number of different mammalian species, but remain poorly characterised the chicken. In this study, we use previously chicken cDC specific reagents, novel gene-edited line single-cell RNA sequencing (scRNAseq) to characterise splenic cDCs. contrast mammals, scRNAseq analysis indicates spleen contains single, chemokine receptor XCR1...
Coccidiosis in poultry, caused by protozoan parasites of the genus Eimeria, is an intestinal disease with substantial economic impact. With use anticoccidial drugs under public and political pressure, comparatively higher cost live-attenuated vaccines, attractive complementary strategy for control to breed chickens increased resistance Eimeria parasitism. Prior infection maxima leads complete immunity against challenge homologous strains, but only partial antigenically diverse heterologous...
A new member of the chicken TNF superfamily has recently been identified, namely receptor activator NF-κB ligand (RANKL), as have its signalling receptor, RANK, and decoy osteoprotegerin (OPG). In mammals, RANKL RANK are transmembrane proteins expressed on surface Th1 cells dendritic (DC) respectively, whereas OPG is a soluble protein from osteoblasts DC. Recombinant (chRANKL) forms homotrimers (chOPG) homodimers, characteristic these molecules in mammals. ChRANKL, chRANK chOPG at mRNA level...
Conventional dendritic cells (cDC) are bone marrow-derived immune that play a central role in linking innate and adaptive immunity. cDCs efficiently uptake, process present antigen to naïve T cells, driving clonal expansion of antigen-specific T-cell responses. In chicken, vital reagents lacking for the efficient precise identification cDCs. this study, we have developed several novel characterization chicken Chicken FLT3 cDNA was cloned monoclonal antibody cell surface generated. This...
Summary T‐cell immunoglobulin and mucin ( TIM ) family molecules are cell membrane proteins, preferentially expressed on various immune cells implicated in recognition clearance of apoptotic cells. Little is known their function outside human mouse, nothing mammals. We identified only two genes ch the chicken genome, putative orthologues mammalian 1 4 , cloned respective cDNA s. Like expression was restricted to lymphoid tissues The gene encodes at least five splice variants with distinct...
Abstract The chicken immune system and microbiota play vital roles in maintaining gut homeostasis protecting against pathogens. In mammals, XCR1+ conventional dendritic cells (cDCs) are located the gut-draining lymph nodes a major role homeostasis. These cDCs sample antigens luminal contents limit inflammatory response to commensal microbes by generating appropriate regulatory effector T-cell responses. We hypothesised that these similar sustaining chickens, chickens lacking XCR1 were likely...