A5088579371- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Animal Nutrition and Physiology
- Veterinary medicine and infectious diseases
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Plant Pathogenic Bacteria Studies
- Animal Virus Infections Studies
- Glycosylation and Glycoproteins Research
- Antimicrobial Resistance in Staphylococcus
Roslin Institute
2021-2025
University of Edinburgh
2021-2025
University of Essex
2019-2020
Effective mucosal immunity in the intestine involves a fine balance between tolerance of microbiome, recognition and elimination pathogens, inflammatory tissue injury. The anti-inflammatory cytokine IL10 regulates these processes intestines mice humans; activity is also conserved birds. To determine function avian immunity, we generated germ line modifications chicken locus to abolish or reduce expression. In vitro analysis macrophage response lipopolysaccharide confirmed loss protein...
Active efflux due to tripartite RND pumps is an important mechanism of clinically relevant antibiotic resistance in Gram-negative bacteria. These are also essential for pathogens cause infection and form biofilms. They consist inner membrane transporter; a periplasmic adaptor protein (PAP), outer channel. The role PAPs assembly, the identities specific residues involved PAP-RND binding, remain poorly understood. Using recent high-resolution structures, four 3D sites binding within each PAP...
Campylobacter is a major cause of acute gastroenteritis in humans, and infections can be followed by inflammatory neuropathies other sequelae. Handling or consumption poultry meat the primary risk factor for human campylobacteriosis, C. jejuni remains highly prevalent retail chicken many countries. Control avian reservoir expected to limit incidence disease. Toward this aim, we evaluated glycoconjugate vaccine comprising fibronectin-binding adhesin FlpA conjugated up ten moieties conserved...
Abstract Background Poultry is the world's most popular animal-based food and global production has tripled in past 20 years alone. Low-cost vaccines that can be combined to protect poultry against multiple infections are a current imperative. Glycoconjugate vaccines, which consist of an immunogenic protein covalently coupled glycan antigens targeted pathogen, have proven track record human vaccinology, but yet used for livestock due prohibitively high manufacturing costs. To overcome this,...
Campylobacter jejuni is a leading global cause of bacterial gastroenteritis in humans, and poultry are major reservoir. Glycoconjugate vaccines containing the conserved C. N-glycan have previously been reported to be effective at reducing caecal colonisation chickens by jejuni. These include recombinant subunit vaccines, live E. coli strains expressing on surface as well outer membrane vesicles (OMVs) derived from these strains. In this study, we evaluated efficacy plasmid glycosylated OMVs...
Abstract Background Worldwide, an estimated 70.7 billion broilers were produced in 2020. With the reduction use of prophylactic antibiotics as a result consumer pressure and regulatory oversight alternative approaches, such vaccination, are required to control bacterial infections. A potential way produce multivalent vaccine is via generation glycoconjugate which consists antigenic protein covalently linked immunogenic carbohydrate. Protein-glycan coupling technology (PGCT) approach generate...
The resistance-nodulation-division (RND) family of efflux pumps confer clinically relevant antibiotic resistance in Gram-negative bacteria, such as Salmonella enterica . RND pumps, including AcrB, are organized tri-partite systems, consisting an inner membrane pump, a periplasmic adaptor protein (PAP) and outer channel. Previously, inactivation the PAPs AcrA AcrE S. has been shown to significantly increase susceptibility antimicrobials reduce virulence. Therefore, seen attractive targets for...