We evaluated bioflocculant production by a freshwater actinobacteria whose 16S rDNA nucleotide sequence was deposited in GenBank as Streptomyces sp. Gansen (accession number HQ537129). Optimum culture conditions for were an initial medium pH of 6.8, incubation temperature 30 °C, agitation speed 160 rpm and inoculum size 2% (v/v) cell density 1.5 × 108 cfu/mL. The carbon, nitrogen cation sources optimum glucose (89% flocculating activity), ammonium sulfate (76% activity) MgCl2. Bioflocculant...

The discharge of poorly treated effluents into the environment has far reaching, consequential impacts on human and aquatic life forms. Thus, we evaluated flocculating efficiency our test bioflocculant report for first time ability biopolymeric flocculant produced by Arthrobacter humicola in treatment sewage wastewater. This strain was isolated from sediment soil sample at Sterkfontein dam Eastern Free State province South Africa.Basic Local Alignment Search Tool (BLAST) analysis nucleotide...

A bacterium isolated from Sterkfontein dam was confirmed to produce bioflocculant with excellent flocculation activity. The 16S rDNA nucleotide sequence analyses revealed the bacteria have 99% similarity Streptomyces platensis strain HBUM174787 and deposited in Genbank as accession number FJ 486385.1. Culture conditions for optimal production of included glucose a sole carbon source, resulting flocculating activity 90%. Other included: peptone nitrogen source; presence Mg2+ cations inoculum...

This study evaluated the potential of a biopolymeric flocculant produced by Terrabacter sp. isolated from Sterkfontein Dam, South Africa. Microbial flocculants aid aggregation suspended solutes in solutions, thus, suggesting its alternative application to inorganic and synthetic organic flocculants, which are associated with health-related problems. The 16S rDNA analysis revealed bacteria have 98% similarity MUSC78T sequence was deposited Genbank as accession number KF682157.1. A series...

This study was designed to evaluate the potential of bioflocculant producing strains isolated from wastewater sludge. According Plackett-Burman design, response surface revealed glucose, magnesium sulfate, and ammonium sulfate as critical media components nutritional source, whereas central composite design affirmed an optimum concentration source 16.0 g/l (glucose), 3.5 heptahydrate (MgSO4.7H2O), 1.6 ( (NH4)2SO4), yielding optimal flocculation activity 96.8%. Fourier Transformer Infrared...

We evaluated the bioflocculant production potential of an Actinobacteria, which was isolated from a freshwater environment in Eastern Cape province South Africa. 16S rDNA nucleotide sequencing analyses revealed that actinobacteria belongs to Brachybacterium genus, and sequences were deposited GenBank as sp. UFH, with accession number HQ537131. Optimum fermentation conditions for by bacteria include initial medium pH 7.2, incubation temperature 30 °C, agitation speed 160 rpm inoculum size 2%...

Extracellular rhodanese obtained from Aureobasidium pullulans was employed in both free and immobilized forms for the biodegradation of cyanide present cassava processing mill effluent (CPME). Crosslinking with glutaraldehyde (at an optimum concentration 5% v/v) before entrapment alginate beads resulted highest immobilization yield 94.5% reduced enzyme leakage 1.8%. Rhodanese by cross-linking (cbe) retained about 46% its initial activity after eight cycles catalysis compared to alone (eaa)...

Background: Staphylococcus aureus causes variety of infections in humans and animals worldwide predominates surgical wound infections.This study assessed the antimicrobial potential stem bark extract Chrysophyllum albidum against an array vancomycin resistant (VRSA) isolated from clinical samples.Methods: The methanolic crude plant was preliminary screened for presence phytochemicals; after then, partitioned into n-hexane, chloroform, ethyl acetate butanol fractions.A range concentrations...

The bioactive compounds produced by actinobacteria have played a major role in antimicrobials, bioremediation, biofuels, enzymes, and anti-cancer activities. Biodegradable microbial flocculants been bacteria, algae, fungi. Microbial bioflocculants also attracted biotechnology importance over chemical as result of degradability environmentally friendly attributes they possess. Though, freshwater explored bioflocculation. Yet, there is paucity information on the application isolated from...

The study investigated the wound healing potential of leaf extract Terminalia avicenniodes in vivo models Wistar rats. antimicrobial plant was first determined against a panel pathogens associated with infection using agar-well dilution method. Thirty healthy male rats weighing 180±2.8 g were anaesthetized, incision and excision made on animal aseptically. wounds aseptically infected standardized inoculums Staphylococcus aureus Pseudomonas aeruginosa respectively. Two concentrations ointment...

Trilepisium madagascariense fruits are carbohydrate-rich and this study directly fermented the fruit wastes into bioethanol without need for nutrient supplementation. The total reducing sugar (TRS) present in mesocarp seed of T. (Tmfw) was to using Aureobasidium pullulans. Bioethanol production by A. pullulans also optimized Box-Behnken response surface methodology (RSM). TRS Tmfw were 11.2 ± 0.8 17.1 1.2 g/L, respectively further hydrolysis with cellulase resulted increased indicating...

Objective: Investigations were carried out on cidal effects of crude extract and fractions obtained from stem bark Psidium guajava against a panel 74 strains Escherichia coli Shigella dysenteriae implicated in diarrhea dysentery infections.
 Methods: Powdered sample the was extracted methanol/distilled water (3:2) then partitioned into different organic solvents. The subjected to antibacterial tests bacterial strains. minimum inhibitory concentrations (MIC) bactericidal (MBC) determined...

This study investigated antimicrobial properties of Alchornea cordifolia leaf extract against a panel selected bacterial strains. It also assessed antioxidant and phytochemical the plant constituents responsible for bioactive actions recorded. The potentials crude along with fractions obtained were determined using agar well-diffusion method. most active fraction was partially purified by combination thin-layer column chromatography; activity resulting samples tested results showed that A....

Introduction: In spite of the commendable flocculating activities microbial flocculants, a good number them potentiate significant toxicity. This study evaluated in vivo toxicological implications treatment with Purified bioflocculant (PB) from Arthrobacter humicola using OECD guidelines. Materials and Methods: acute toxicity assessment, single oral administration 2000 mg/kg body weight (b.w.) PB was given to Wistar rats animals were observed for 2 weeks. The daily dose testing performed...

Abstract Tyrosinase (7.9 U/mg protein) from the banana peel ( TBp ) served as a low-cost immobilized biocatalyst for production of 3, 4-di hydroxyphenyl-L-alanine (L-DOPA). L-DOPA, dopamine precursor, useful in management Parkinson’s disease, was produced improvised batch and packed bed reactors. L-DOPA by optimized via response surface methodology (RSM). characterized spectrophotometry (Arnow’s method) thin-layer chromatography. productivities obtained at equimolar substrate concentrations...