Abstract Alzheimer’s disease (AD) is characterized by the accumulation of amyloid beta plaques and neurofibrillary tangles composed hyperphosphorylated tau protein. This study computationally investigated natural neem compounds (limonoids) gut microbiome metabolites for their inhibitory potential against key AD targets. Molecular docking analyses were performed on approximately 200 phytochemicals 9 microbial beta-secretase 1 (BACE1), gingipain cysteine protease, oligomerization receptors...

This study investigated the screening, production and optimization of an extracellular lipase from a fungus isolated contaminated soil palm oil processing shed.This was with view to obtaining strain that can secrete biochemical properties exploitable for biotechnological applications such as bioremediation sites.Soil samples were collected sites in Gbogan, Osun State, Nigeria (Latitude N 7°29.1481´and Longitude E 4°20.7587´).The fungal strains screened on tributyrin agar exogenous lipolytic...

Strain improvement of a low fructosyltransferase-producing Aureobasidium pullulans NAC8 (Accession No. KX023301) was carried out using chemical mutagens such as ethidium bromide and ethyl methane sulfonate. The wild-type mutant strain were distinguished Random amplified polymorphic DNA PCR fingerprinting analysis. Plackett-Burman Box Behnken design statistical tools used to determine important media parameters optimization, respectively. Phenotypically genetically, the new improved different...

Cellulase enzyme was purified and characterized from termite soldiers (Ametermes eveuncifer) using 70% ammonium sulphate precipitation, ion exchange chromatography affinity chromatography. The isolated had a specific activity of 5.04 U/mg with percentage yield 11.7%. showed maximum at 500C pH 8. not inhibited by Ba2+ concentration 1mM Pb2+ 10 mM but other metal ions 1 concentrations their salts (NaCl, KCl, MnCl2, NiCl2),

Pseudothermotoga hypogea is an extremely thermophilic bacterium capable of growing at 90 °C and producing ethanol, which catalyzed by alcohol dehydrogenase (ADH). The gene encoding P. ADH (PhADH) was cloned, sequenced over-expressed. sequence (1164 bp) obtained sequencing all fragments the gene, were amplified from genomic DNA. deduced amino acid showed high identity to iron-containing ADHs other Thermotoga species harbored typical iron- NADP-binding motifs, Asp195His199His268His282...

The research paper investigates the phytochemicals of watermelon (Citrullus lanatus) as potential novel inhibitors phosphodiesterase type 5 (PDE5), which is essential for treating erectile dysfunction and pulmonary arterial hypertension. A total 150 compounds were screened against five PDE5 enzyme structures using molecular docking techniques with Chimera AutoDock Vina. Interactions visualized PyMOL, while SwissADME was employed to predict pharmacokinetic properties, ProTox-II utilized...

ENWEndNote BIBJabRef, Mendeley RISPapers, Reference Manager, RefWorks, Zotero AMA Ademakinwa A, Ayinla Z, Omitogun O, Agboola F. Preparation, characterization and optimization of cross-linked fructosyltransferase aggregates for the production prebiotic fructooligosaccharides. BioTechnologia. 2018;99(4):417-434. doi:10.5114/bta.2018.79972. APA Ademakinwa, A., Ayinla, Z., Omitogun, O., & Agboola, (2018). BioTechnologia, 99(4), 417-434. https://doi.org/10.5114/bta.2018.79972 Chicago Adedeji N.,...

The characteristics of a purified lipase from Rhizopus oryzae ZAC3 (RoL-ZAC3) were investigated. RoL-ZAC3, 15.8 kDa protein, which was optimally active at pH 8 and 55 °C had half-life 126 min 60 °C. kinetic parameters using p-nitrophenylbutyrate as substrate 0.19 ± 0.02 mM, 5.6 U/ml 122 s−1 for Km, Vmax kcat respectively. RoL-ZAC3 showed stability in methanol isopropanol with Na+ enhancing the activity. p-nitrophenyloleate castor oil best preferred substrates among p-nitrophenyl esters...

Elucidation of lipase–substrate interactions will guide the proper industrial use and applicability enzyme. The aim this study was to predict 3 D structure Rhizopus oryzae ZAC3 (RoZAC3) lipase, its with some natural substrates evaluate feasibility fatty acid methyl esters (FAME) production by immobilized lipase. Protein identification RoZAC3 lipase carried out using LC-MS/MS. built homology modelling such as tributyrin, tripalmitin triolein were docked optimized model for investigation...

Rhodanese a ubiquitous sulphur transferase enzyme catalyses the detoxification of cyanide to less toxic thiocyanate. from root Pentadiplandra brazzeana was purified by ammonium sulphate precipitation and affinity chromatography. The had specific activity 4.82 RU/mg protein. Km values substrates (KCN Na2S2O3) were 11.76 10 mM, respectively. optimum pH temperature 8.0 60°C, not inhibited heavy metals (BaCl2, KCl, NiCl2, NaCl, MnCl2, ZnCl2). This study affirmed presence rhodanese in P....

<title>Abstract</title> This study examines the enzymatic profiles, biochemical characteristics, morphological traits, and molecular identification of ten Streptomyces isolates from soil samples in Kogi State, Nigeria, with an emphasis on their potential biodegradation environmental biotechnology. Enzymatic assays revealed that isolate S4 exhibited highest cellulase activity (12 mm zone clearance), while S3 showed significant ligninase (absorbance 0.85 at 530 nm). Notably, S7 demonstrated...

<title>Abstract</title> Background This study investigates the optimal growth conditions for various Streptomyces isolates, focusing on temperature, pH, and incubation period. Additionally, it assesses their antioxidant cytotoxic potential to explore industrial therapeutic applications. Methods The of isolates were evaluated by varying Temperature preferences tested from 30°C 60°C, while pH was adjusted between 5.0 7.5. Growth dynamics monitored, with peaks identified 48 120 hours....

Abstract Background β-Cyanoalanine synthase plays essential roles in germinating seeds, such as cyanide homeostasis. Methods was isolated from sorghum purified using chromatographic techniques and its biochemical catalytic properties were determined. Results The enzyme had a yield of 61.74% specific activity 577.50 nmol H 2 S/min/mg protein. apparent subunit molecular weight for β-cyanoalanine 58.26±2.41 kDa 63.4 kDa, respectively. kinetic parameters with sodium substrate 0.67±0.08 mM,...

Rhodanese enzyme was partially purified and characterized from seed mesocarp of snake tomato (Trichosanthes cucumerina) using 80% ammonium sulphate precipitation. The isolated T. cucumerina had a specific activity 8.8 RU/mg with percentage yield 87.8%, while that 19.7 14.6%. Km rhodanese for sodium thiosulphate (Na2S2O3) potassium cyanide (KCN) were 12.5 10 mM, respectively. both not inhibited by persulphate metabisulphite. optimum temperature the 50&deg;C, pH 8.0 7.0, metal ions at...

Abstract Aureobasidium pullulans α-amylase ( ApAmy ) mixed with melted agar-agar solution and drop-wisely added to a mixture of organic solvent allowed for the entrapment in matrix as beads. The immobilized ’s characteristics wash performance were elucidated comparison soluble . Agar-agar at 2.0 % (w/v) toluene: chloroform 3:1 resulted highest immobilization yield retaining about 98% residual activity after ten catalytic cycles. optimum temperature pH enzyme 60ºC 6.5 respectively. hydrolysed...

Abstract This study was undertaken to isolate and identify a novel cellulase-producing strain from waste site (7°28’11’’N 4°31’24’’E), optimise the growth conditions, partially purify biochemically characterise enzyme. The potentials of purified cellulase hydrolyse lignocellulosic component some agro-industrial wastes (e.g. orange peels etc.) also investigated. best fungus identified as Mucor ramanniacus optimum conditions for production were pH (4.5), inoculum size (12 mm), carbon nitrogen...