A5083046798- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Viral Infectious Diseases and Gene Expression in Insects
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- RNA Research and Splicing
- Hormonal Regulation and Hypertension
- Bone Tissue Engineering Materials
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Protein purification and stability
- Collagen: Extraction and Characterization
- Dendrimers and Hyperbranched Polymers
- Proteoglycans and glycosaminoglycans research
- Cancer, Hypoxia, and Metabolism
- Testicular diseases and treatments
- Nitric Oxide and Endothelin Effects
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Cancer, Lipids, and Metabolism
University of Central Florida
2019-2025
University of Houston - Clear Lake
2024
Prostate cancer is the leading in incidence and second cause of mortality US men. African American men have significantly higher rates from prostate than European Previous studies reported that disparity survival or can be explained by different biological backgrounds. microRNAs (miRNAs) regulate gene expression their cognate mRNAs many cancers. Therefore, miRNAs may a potentially promising diagnostic tool. The role aggressiveness racial has not been fully established. goal this study to...
Abstract Long non-coding RNAs (lncRNAs) modulate crucial cellular processes in nexus with miRNAs, transcription factors and RNA-binding proteins (RBPs) whose dysregulation is often linked cancer progression drug resistance. In aggressive, recurrent fatal invasive cancers, there an unmet need to identify newer targets their mechanistic roles for improved therapies. Previously, we reported association of lncRNA PAINT aggressive prostate (PCa) its involvement dysregulated expression several...
Abstract Prostate cancer (PCa) is one of the most common cancers to affect men worldwide. Androgen receptor (AR) signaling central PCa and therapy. MicroRNAs (miRNAs) play crucial roles in regulation prostate through modulation pathways. In present study, we illustrate functional significance therapeutic benefit miR-299-3p, an AR targeting microRNA, progression. We noted loss expression miR-299-3p tumors compared noncancerous tissues. Replenishment C4-2B, 22Rv-1 PC-3 cells contributed cell...
Prostate cancer (PCa) progression relies on androgen receptor (AR) function, making AR a top candidate for PCa therapy. However, development of drug resistance is common, which eventually leads to castration-resistant PCa. This warrants better understanding the pathophysiology that facilitates aberrant activation key signaling pathways including AR. MicroRNAs (miRNAs) function as regulators they modulate various cellular processes. Here, we demonstrate multidimensional miR-30e through...
Peroxidative damage to human spermatozoa has been shown be the primary cause of male infertility. The possible role nitric oxide (NO) in affecting sperm motility, capacitation, and acrosome reaction reported, too. overproduction NO by enzyme inducible synthase (iNOS) could responsible as it implicated pathogenesis many diseases. There have studies on regulating iNOS function various tissues, especially protein–protein interaction; however, no study looked for iNOS-interacting proteins...
Abstract Long noncoding RNAs (lncRNAs) play regulatory role in cellular processes and their aberrant expression may drive cancer progression. Here we report the function of a lncRNA PAINT (prostate associated intergenic transcript) promoting prostate (PCa) Upregulation was noted advanced stage metastatic PCa. Inhibition decreased cell proliferation, S‐phase progression, increased apoptotic markers, improved sensitivity to docetaxel Aurora kinase inhibitor VX‐680. migration reduced Slug...
Abstract Chinese hamster ovary (CHO) cells are the preferred system for expression of therapeutic proteins and majority all biotherapeutics being expressed by these cell lines. CHO systems readily scalable, resistant to human adventitious agents, have desirable post‐translational modifications, such as glycosylation. Regardless, drug development a whole is very costly, complicated, time‐consuming process. Therefore, any improvements that result in reducing timelines valuable can provide...
ABSTRACT Targeted integration (TI) Chinese hamster ovary (CHO) platforms are commonly used for protein expression. However, the impact of epigenetic modifications on expression in TI cell lines remains elusive since almost all studies focus random (RI) gene interest and only within promoter region. To address CHO cells, we utilized a standard mAb‐1 to identify characterize clones with same transgene copy numbers but different levels transcription titer. Surprisingly, while CMV promoters were...
Abstract Long non-coding RNAs (lncRNAs) play regulatory roles in cellular processes and their aberrant expression may drive cancer progression. Here we report the function of a lncRNA PAINT (Prostate Cancer Associated Intergenic Non-Coding Transcript) promoting prostate (PCa) Upregulation was noted advanced stage metastatic PCa. Inhibition decreased cell proliferation, S-phase progression, increased apoptotic markers, improved sensitivity to docetaxel Aurora kinase inhibitor VX-680....
Abstract Promoter hypermethylation is one of the events that downregulate microRNAs (miRNA), resulting in differential expression genes and progression cancer. Previously we showed down-regulation two miRNAs, miR-299-3p -30e target androgen receptor (AR) advanced prostate cancer (PCa), their function as tumor suppressors. In this study, examined contributing for loss these miRNAs if varies PCa tissues cells from racially disparate group patients. To test whether promoter reasons, effect...
Abstract MicroRNAs (miRNAs) play crucial roles in the regulation of prostate cancer (PCa) through modulation signaling pathways. Androgen receptor (AR) is central to PCa and therapy. The therapeutic suitability miRNAs that target AR has not been investigated thoroughly. Here, we illustrate clinical relevance, functional significance benefit miR-299-3p, an targeting microRNA, progression. We noted a loss expression miR-299-3p tumors compared noncancerous tissue specimens. Studies on impact...
Abstract Long non-coding RNAs (LncRNAs) play regulatory roles in cellular processes and their aberrant expression may drive cancer progression. Here we report the function of a LncRNA PAINT (Prostate Cancer-Associated Intergenic Non-Coding Transcript) promoting prostate (PCa) Upregulation was noted advanced stage metastatic PCa patient tissues correlated with poor survival. Inhibition decreased cell proliferation, S-phase progression, increased apoptotic markers, improved sensitivity to...