A5045554961- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Protein purification and stability
- PARP inhibition in cancer therapy
- Viral Infectious Diseases and Gene Expression in Insects
- Phytochemicals and Medicinal Plants
- Pharmacogenetics and Drug Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Calcium signaling and nucleotide metabolism
- Drug Transport and Resistance Mechanisms
- SARS-CoV-2 detection and testing
- Chemical Synthesis and Analysis
- Enzyme Catalysis and Immobilization
- Spectroscopy Techniques in Biomedical and Chemical Research
- vaccines and immunoinformatics approaches
- Drug-Induced Hepatotoxicity and Protection
Budapest University of Technology and Economics
2022-2024
HUN-REN Research Centre for Natural Sciences
2017
Hungarian Academy of Sciences
2017
Institute of Molecular Life Sciences
2017
This research shows the detailed comparison of Raman and near-infrared (NIR) spectroscopy as Process Analytical Technology tools for real-time monitoring a protein purification process. A comprehensive investigation application model development NIR was carried out process-related impurity, imidazole, during tangential flow filtration Receptor-Binding Domain (RBD) SARS-CoV-2 Spike protein. The fast spectroscopy-based calibration models achieved using offline data, resulting in low...
To date, there are limited options for severe Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 virus. As ADP-ribosylation events involved in regulating the life cycle of coronaviruses and inflammatory reactions host; we have, here, assessed repurposing registered PARP inhibitors treatment COVID-19.
Abstract Cariprazine—the only single antipsychotic drug in the market which can handle all symptoms of bipolar I disorder—involves trans- 4-substituted cyclohexane-1-amine as a key structural element. In this work, production trans -4-substituted cyclohexane-1-amines was investigated applying transaminases either diastereotope selective amination starting from corresponding ketone or diastereomer deamination their diasteromeric mixtures. Transaminases were identified enabling conversion cis...
We have developed a simple, rapid, high-throughput RBD-based ELISA to assess the humoral immunity against emerging SARS-CoV-2 virus variants. The cDNAs of his-tagged RBD proteins variants were stably engineered into HEK cells secreting protein supernatant, and purification was performed by Ni-chromatography buffer exchange membrane filtration. simplified assay uses single dilutions sera from finger-pricked native blood samples, purified in 96-well plates, chromogenic dye for development....
We have developed a simple, rapid, high-throughput RBD-based ELISA to assess the humoral immunity against emerging SARS-CoV-2 virus variants. The cDNAs of His-tagged RBD proteins variants were stably engineered into HEK cells secreting protein supernatant, and purification was performed by Ni-chromatography buffer exchange membrane filtration. simplified assay uses single dilutions sera from finger-pricked native blood samples, purified in 96-well plates, chromogenic dye for development....
Abstract Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2 virus, is a major global health challenge, as there no efficient treatment for the moderate to severe disease. ADP-ribosylation events are involved in regulating life cycle of coronaviruses and inflammatory reactions host, hence we assessed repurposing registered PARP inhibitors COVID-19. We detected high levels oxidative stress strong PARylation all cell types lungs COVID-19 patients. Interestingly, rucaparib, unlike other...
Protein A affinity chromatography is a well-established method currently used in the pharmaceutical industry. However, high costs usually associated with chromatographic separation of protein and difficulties continuous operation make investigation alternative purification methods very important.In this study, extraction/back-extraction precipitation/dissolution were developed optimised. They compared cation exchange separations terms yield monoclonal antibody (mAb) amount residual...