A5038038706- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Metastasis and carcinoma case studies
- Extracellular vesicles in disease
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Respiratory viral infections research
- Advanced biosensing and bioanalysis techniques
- Hippo pathway signaling and YAP/TAZ
- Transplantation: Methods and Outcomes
- Inhalation and Respiratory Drug Delivery
- Esophageal Cancer Research and Treatment
- Lipid Membrane Structure and Behavior
- Advanced Biosensing Techniques and Applications
- Management of metastatic bone disease
- T-cell and B-cell Immunology
- Tissue Engineering and Regenerative Medicine
- Hematopoietic Stem Cell Transplantation
- Sarcoma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Gastric Cancer Management and Outcomes
- SARS-CoV-2 and COVID-19 Research
Georgia Institute of Technology
2023-2024
The Wallace H. Coulter Department of Biomedical Engineering
2023-2024
University of Arizona
2018-2021
University of Phoenix
2018
Adding a cationic helper lipid to nanoparticle (LNP) can increase lung delivery and decrease liver delivery. However, it remains unclear whether charge-dependent tropism is universal or, alternatively, depends on the component that charged. Here, we report evidence cholesterol-dependent differ from lipid-dependent tropism. By testing how 196 LNPs delivered mRNA 22 cell types, found charged cholesterols led different lung:liver ratio than lipids. We also combining cholesterol with in heart as...
Lipid nanoparticles (LNPs) have delivered RNA to hepatocytes in patients, underscoring the potential impact of nonliver delivery. Scientists can shift LNP tropism lung by adding cationic helper lipids; however, biological response these LNPs remains understudied. To evaluate hypothesis that charged lead differential cellular responses, we quantified how 137 mRNA 19 cell types vivo. Consistent with previous studies, observed lipid-dependent tropism. After identifying and individually...
Most cancer patients diagnosed with late-stage head and neck squamous cell carcinoma are treated chemoradiotherapy, which can lead to toxicity. One potential alternative is tumor-limited conversion of a prodrug into its cytotoxic form. We reason this could be achieved by transient tumor-specific expression purine nucleoside phosphorylase (PNP), an Escherichia coli enzyme that converts fludarabine 2-fluoroadenine, potent drug. To efficiently express bacterial PNP in tumors, we evaluate 44...
Plant-based scaffolds present many advantages over a variety of biomaterials. Recent studies explored their potential to be repopulated with human cells and thus highlight growing interest for use in tissue engineering or biomedical applications. However, it is still unclear if these vitro plant-based can modify cell phenotype affect behavior external stimuli. Here, we report the characterization mechano-regulation melanoma SK-MEL-28 prostate PC3 seeded on decellularized spinach leaves...
RNA therapeutics are promising for the treatment of lung diseases where small molecules and biologics have failed to much success in past. While siRNA mRNA drugs now FDA-approved rare liver as vaccines infectious diseases, they became clinically established only after suitable advances drug delivery platforms, such case lipid nanoparticles. Localized through nanocarriers optimized inhaled administration has potential address respiratory without toxicity concerns from off-target effects....
Abstract Current in vitro tumor models have issues accuracy that the 2D structures and (often rare) cell co-culture technologies exist, lack many features or characteristics found vivo. The use of decellularized plant recellularized with human cells, aims to overcome these by taking advantage their natural 3D structure. By using this approach on spinach leaves as a scaffold, we developed new model may be used for radiobiology research. Spinach were following serial chemical treatments...