A5030704809- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Genetic Syndromes and Imprinting
- Cancer-related molecular mechanisms research
- Chromosomal and Genetic Variations
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Cerebral Palsy and Movement Disorders
- Genetic Neurodegenerative Diseases
- Nigella sativa pharmacological applications
- Plant nutrient uptake and metabolism
- Viral Infections and Outbreaks Research
- RNA regulation and disease
- Stroke Rehabilitation and Recovery
- Drug-Induced Adverse Reactions
- Vaccine Coverage and Hesitancy
- Bacillus and Francisella bacterial research
- Erythrocyte Function and Pathophysiology
- Genomic variations and chromosomal abnormalities
University of Agriculture Faisalabad
2025
Countess of Chester Hospital NHS Foundation Trust
2023
Countess of Chester Hospital
2023
University of Liverpool
2023
Peterborough City Hospital
2023
Johannes Gutenberg University Mainz
2018-2022
University of Sargodha
2018-2021
University of Health Sciences Lahore
2019-2021
Dow University of Health Sciences
2019
University of Haripur
2019
Divergent morphology of species has largely been ascribed to genetic differences in the tissue-specific expression proteins, which could be achieved by divergence cis-regulatory elements or altering binding specificity transcription factors (TFs). The relative importance latter difficult assess, as previous systematic analyses TF have performed using different methods species. To address this, we determined specificities 242 Drosophila TFs, and compared them human mouse data. This analysis...
Abstract Background Advances in sequencing technology have boosted population genomics and made it possible to map the positions of transcription factor binding sites (TFBSs) with high precision. Here we investigate TFBS variability by combining maps generated ENCODE, modENCODE, our previously published data other sources genomic variation for human individuals Drosophila isogenic lines. Results We introduce a metric that takes into account changes motif match associated mutation makes...
The exon junction complex (EJC) is a highly conserved ribonucleoprotein that binds RNAs during splicing and remains associated with them following export to the cytoplasm. While role of this in mRNA localization, translation, degradation has been well characterized, its mechanism action subset Drosophila human transcripts be elucidated. Here, we describe novel function for EJC subunit, RnpS1, preventing transposon accumulation both germline surrounding somatic follicle cells. This mediated...
Abstract Promoter-proximal pausing of RNA polymerase II (Pol II) is a widespread transcriptional regulatory step across metazoans. Here we find that the nuclear exon junction complex (pre-EJC) critical and conserved regulator this process. Depletion pre-EJC subunits leads to global decrease in Pol premature entry into elongation. This effect occurs, at least part, via non-canonical recruitment components promoters. Failure recruit promoters results increased binding positive transcription...
Sensory systems sequentially extract increasingly complex features. ON and OFF pathways, for example, encode increases or decreases of a stimulus from common input. This ON/OFF pathway split is thought to occur at individual synaptic connections through sign-inverting synapse in one the pathways. Here, we show that selectivity multisynaptic process Drosophila visual system. A pharmacogenetics approach demonstrates both glutamatergic inhibition GluClα GABAergic Rdl mediate responses. Although...
Magnesium (Mg) is essential for plant growth and development plays critical roles in physiological biochemical processes. Mg deficiency adversely affects of plants by limiting shoot root development, disturbing the structure membranes grana, reducing photosynthesis efficiency, lowering net CO2 assimilation. The MGT (Magnesium transporter) family responsible absorption transportation magnesium plants. Although has been identified different species, research regarding soybean genes limited. In...
Abstract Mutations in the actively expressed, maternal allele of imprinted KCNK9 gene cause Birk-Barel intellectual disability syndrome (BBIDS). Using a BBIDS mouse model, we identify here partial rescue BBIDS-like behavioral and neuronal phenotypes mediated via residual expression from paternal Kcnk9 ( pat ) allele. We further demonstrate that second-generation HDAC inhibitor CI-994 induces enhanced paternally silenced leads to full phenotype suggesting as promising molecule for therapy....
Coronavirus disease 2019 (COVID‐19) is a novel respiratory that has led to global pandemic and created havoc. The COVID‐19 severity varies among individuals, depending on fluctuating symptoms. Many infectious diseases such as hepatitis B dengue hemorrhagic fever have been associated with ABO blood groups. aim of this study was explore whether groups might serve risk or protective factor for infection. Moreover, the symptomatic variations infection individuals different were also analyzed. An...
Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions cells generate results with high signal-to-noise ratio precludes it in small cell populations. Here, we present tagmentation-assisted fragmentation ChIP (TAF-ChIP) and method high-quality histone profiles from low numbers. The data obtained TAF-ChIP approach are amenable standard tools for ChIP-Seq analysis,...
Dual-use research poses a significant challenge for scientists in the biomedical field and global health security general. As scientific knowledge materials required development of biological agents become progressively more accessible inexpensive, there is an increased need to understand improve governance research. Prevention misuse facilities, equipment, agents, requires high levels awareness concept dual-use research, starting with early-career graduate students. In this study, attitudes...
Abstract RNA Polymerase II (RNAP II) pausing is essential to precisely control gene expression and critical for development of metazoans. Here, we show that the m 6 A modification regulates promoter-proximal RNAP pausing. The methyltransferase complex (MTC), with nuclear reader Ythdc1, are recruited promoters. Depleting MTC leads a decrease in pause release Ser2P occupancy on body, affects nascent transcription. Tethering Mettl3 heterologous promoter sufficient increase release, an effect...
We present the case of a 28-year-old gentleman who presented with weakness and wasting in right arm. He complained about painful cramps his left leg but there were no sensory, visual or swallowing problems. Neurological examination was significant for asymmetric both upper lower limbs deep tendon reflexes asymmetrically brisk. Initial laboratory investigations, MRI brain spine normal. Electromyography revealed active denervation chronic neurogenic motor unit action potential. Myokymic...
Abstract Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is powerful technique to study transcriptional regulation. However, the requirement of millions cells generate results with high signal-to-noise ratio precludes it in small cell populations. Here, we present a Tagmentation-Assisted Fragmentation ChIP (TAF-ChIP) and method high-quality from low numbers. The data obtained TAF-ChIP approach amenable standard tools for ChIP-Seq analysis, owing its...
Background: Cerebral palsy (CP) encompasses a group of permanent movement and posture disorders caused by early brain damage. It is commonly associated with muscle spasticity, impaired range motion (ROM), reduced mobility. While various pharmacological non-pharmacological treatments exist, the evidence regarding effectiveness myofascial release (MFR) alone or in combination static stretching (SS) reducing spasticity improving ROM children CP remains limited. Objective: This study aimed to...
In this case report, we discuss a young female who presented to the emergency department with recent onset of weakness, paraesthesia, and gait disturbance suggestive peripheral neuropathy ataxia. This was attributed occasional use recreational nitrous oxide (N2O) over past 10 months. Subacute combined degeneration spinal cord is condition affecting lateral posterior columns cord, mainly caused by demyelination. The N2O depletes levels vitamin B12 thus leading demyelination nervous system....
Objectives: To determine the frequency of two common HFE Gene Mutations(C282Y & H63D) in an immigrant population (British Pakistanis) UK. Study Design: Crosssectional study. Setting: University Lincoln Duration: Duration study was 12 monthsfrom 01/09/2012 to 31/08/2013. Material and Methods: Two hundred Pakistani (BP)chromosomes (100 samples; 50 male female) from major cities UK 200 ancestralorigin chromosomes were analysed by PCRRFLPfor presence H63D C282Y mutations. Results: Eight...
SUMMARY Promoter proximal pausing of RNA polymerase II (Pol II) is a widespread transcriptional regulatory step across metazoans. Here we find that the nuclear exon junction complex (pre-EJC) plays critical and conserved role in this process. Depletion pre-EJC subunits leads to global decrease Pol premature entry into elongation. This effect occurs, at least part, via non-canonical recruitment components promoters. Failure recruit promoters results increased binding positive transcription...
Background: Post-surgical rehabilitation of Colle’s fracture requires manual and exercise therapy to restore normal hand function. Aim: To compare the effectiveness Maitland mobilization Mulligan’s with movement on function pain after fracture. Study Design: Non randomized clinical study. Methodology: was conducted ethical approval, at physiotherapy department Mayo hospital Lahore in 2019-20. Sample 60 patients estimated by epitool, divided into three equal groups (A, B & C). Subjects...