A5019557263- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- Ion Transport and Channel Regulation
- Neuroscience and Neural Engineering
- Ion Channels and Receptors
- Receptor Mechanisms and Signaling
- Cardiomyopathy and Myosin Studies
- Pain Mechanisms and Treatments
- Sleep and Wakefulness Research
- Neuroscience of respiration and sleep
- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- Hearing, Cochlea, Tinnitus, Genetics
- Pharmacological Receptor Mechanisms and Effects
- Hormonal Regulation and Hypertension
- Neonatal Respiratory Health Research
- Erythrocyte Function and Pathophysiology
- Cellular transport and secretion
- Nanopore and Nanochannel Transport Studies
- Signaling Pathways in Disease
- Renin-Angiotensin System Studies
- Neuroendocrine regulation and behavior
- Nitric Oxide and Endothelin Effects
Institut de Pharmacologie Moléculaire et Cellulaire
2015-2024
Centre National de la Recherche Scientifique
2014-2023
Inserm
1993-2023
Laboratoire d'Excellence Canaux Ioniques d'Intérêt Thérapeutique
2012-2023
Université Côte d'Azur
2012-2023
Observatoire de la Côte d’Azur
2023
Centre National pour la Recherche Scientifique et Technique (CNRST)
2011-2020
Institut de Biologie Valrose
2014
Centre Hospitalier Intercommunal de Poissy
2013
Hôpital Pasteur
2004-2007
TREK-1 is a member of the novel structural class K+ channels with four transmembrane segments and two pore domains in tandem (1Fink M. Duprat F. Lesage Reyes R. Romey G. Heurteaux C. Lazdunski EMBO J. 1996; 15: 6854-6862Crossref PubMed Scopus (421) Google Scholar, 2Patel A.J. Honoré E. Maingret Fink 1998; 17: 4283-4290Crossref (527) Scholar). opened by membrane stretch arachidonic acid. It also an important target for volatile anesthetics (2Patel 3Patel Nature Neurosci. 1999; 2:...
The two-pore (2P) domain K(+) channels TREK-1 and TRAAK are opened by membrane stretch as well arachidonic acid (AA) (Patel, A. J., Honoré, E., Maingret, F., Lesage, Fink, M., Duprat, Lazdunski, M. (1998) EMBO J. 17, 4283-4290; Patel, E. (1999) Biol. Chem. 274, 26691-26696; Fosset, , 1381-1387. We demonstrate that lysophospholipids (LPs) platelet-activating factor also produce large specific reversible activations of TRAAK. LPs activation is a function the size polar head length acyl chain...
The novel structural class of mammalian channels with four transmembrane segments and two pore regions comprise background K+ (TWIK-1, TREK-1, TRAAK, TASK, TASK-2) unique physiological functions (1Fink M. Lesage F. Duprat Heurteaux C. Reyes R. Fosset Lazdunski EMBO J. 1998; 17: 3297-3308Crossref PubMed Scopus (394) Google Scholar, 2Duprat Fink 1997; 16: 5464-5471Crossref (547) 3Fink Romey G. 1996; 15: 6854-6862Crossref (424) 4Lesage Guillemare E. Barhanin 1004-1011Crossref (457) 5Patel A.J....
A complementary DNA encoding a novel K<sup>+</sup> channel, called TASK-2, was isolated from human kidney and its gene mapped to chromosome 6p21. TASK-2 has low sequence similarity other two pore domain channels, such as TWIK-1, TREK-1, TASK-1, TRAAK (18–22% of amino acid identity), but similar topology consisting four potential membrane-spanning domains. In transfected cells, produces noninactivating, outwardly rectifying currents with activation thresholds that closely follow the...
Mechano-sensitive and fatty acid-activated K+ belong to the structural class of K+channel with two pore domains. Here, we report isolation characterization a novel member this family. This channel, called TREK2, is closely related TREK1 (78% homology). Its gene located on chromosome 14q31. TREK2 abundantly expressed in pancreas kidney lower level brain, testis, colon, small intestine. In central nervous system, has widespread distribution highest levels expression cerebellum, occipital lobe,...
MbIRK3, mbGIRK2 and mbGIRK3 K+ channels cDNAs have been cloned from adult mouse brain. These encode polypeptides of 445, 414 376 amino acids, respectively, which display the hallmarks inward rectifier channels, i.e. two hydrophobic membrane-spanning domains M1 M2 a pore-forming domain H5. MbIRK3 shows around 65% acid identity with IRK1 rbIRK2 only 50% ROMK1 GIRK1. On other hand, are more similar to GIRK1 (60%) than (50%). Northern blot analysis reveals that these three novel clones mainly...
Four cDNA-encoding G-activated inwardly rectifying K+ channels have been cloned recently (Kubo, Y., Reuveny, E., Slesinger, P. A., Jan, Y. N., and L. Y.(1993) Nature 364, 802-806; Lesage, F., Duprat, Fink, M., Guillemare, Coppola, T., Lazdunski, Hugnot, J. (1994) FEBS Lett. 353, 37-42; Krapivinsky, G., Gordon, E. Wickman, K., Velimirovic, B., L., Clapham, D. (1995) 374, 135-141). We report the cloning of a mouse GIRK2 splice variant, noted mGIRK2A. Both channel proteins are functionally...
Riluzole (RP 54274) is a potent neuroprotective agent with anticonvulsant, sedative, and anti-ischemic properties. It currently used in the treatment of amyotrophic lateral sclerosis. This article reports that riluzole an activator TREK-1 TRAAK, two important members new structural family mammalian background K(+) channels four transmembrane domains pore regions. Whereas activation TRAAK sustained, transient followed by inhibition. The inhibitory process attributable to increase...
The present work shows that arachidonic acid and some other long chain polyunsaturated fatty acids such as docosahexaenoic acid, which is abundant in fish oil, produce a direct open channel block of the major voltage-dependent K+ (Kv1.5) cloned cardiac cells. inhibitory action these selected seen when they are applied extracellularly but not included patch pipette. Fatty then appear to bind an external site on Kv1.5 structure. Inhibition activity by (acceleration apparent inactivation...
Free radical-induced oxidant stress has been implicated in a number of physiological and pathophysiological states including ischemia reperfusion-induced dysrhythmia the heart, apoptosis T lymphocytes, phagocytosis, neurodegeneration. We have studied effects on native K+ channel from lymphocytes channels cloned cardiac, brain, T-lymphocyte cells expressed Xenopus oocytes. The activity three Shaker (Kv1.3, Kv1.4, Kv1.5), one Shaw (Kv3.4), inward rectifier (IRK3) was drastically inhibited by...
Mechanosensitive K(+) channels TREK1 and TREK2 form a subclass of two P-domain channels. They are potently activated by polyunsaturated fatty acids involved in neuroprotection, anesthesia, pain perception. Here, we show that acidification the extracellular medium strongly inhibits with an apparent pK near to 7.4 corresponding physiological pH. The all-or-none effect pH variation is steep observed within one unit. not inhibited but same range pH, despite its close homology TREK1. A single...