A5017460289- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Prenatal Substance Exposure Effects
- Biochemical Acid Research Studies
- Birth, Development, and Health
- Mitochondrial Function and Pathology
- Pregnancy and preeclampsia studies
- Endoplasmic Reticulum Stress and Disease
- Epigenetics and DNA Methylation
- Muscle metabolism and nutrition
- Sirtuins and Resveratrol in Medicine
- Glycogen Storage Diseases and Myoclonus
- Genetic Syndromes and Imprinting
- Liver Disease Diagnosis and Treatment
- Cancer-related gene regulation
- Neonatal Health and Biochemistry
- Neuroscience and Neural Engineering
- Cardiovascular Disease and Adiposity
- Cancer, Hypoxia, and Metabolism
- Enzyme Catalysis and Immobilization
- Liver physiology and pathology
- PI3K/AKT/mTOR signaling in cancer
- Alzheimer's disease research and treatments
- Bacterial Infections and Vaccines
Baylor University
2024
Boston Medical Center
2010-2013
Boston University
2010-2012
Dana-Farber Cancer Institute
2011
Tufts University
2011
Diabetes Australia
2011
University Medical Center
2010-2011
Harvard University
2011
The University of Texas at Austin
2008
Rhode Island Hospital
2003-2006
We previously reported that adenosine monophosphate-activated protein kinase (AMPK) activity is lower in adipose tissue of morbidly obese individuals who are insulin resistant than comparably people sensitive. However, the number patients and parameters studied were small. Here, we compared abdominal subcutaneous, epiploic, omental fat from 16 classified as sensitive or based on homeostatic model assessment resistance. confirmed AMPK diminished group. A custom PCR array revealed increases...
OBJECTIVE Branched-chain amino acids, such as leucine and glucose, stimulate protein synthesis increase the phosphorylation activity of mammalian target rapamycin (mTOR) its downstream p70S6 kinase (p70S6K). We examined in skeletal muscle whether effects glucose on these parameters insulin resistance are mediated by fuel-sensing enzyme AMP-activated (AMPK). RESEARCH DESIGN AND METHODS Rat extensor digitorum longus (EDL) was incubated with different concentrations or without AMPK activators....
Gestational exposure to ethanol causes fetal alcohol syndrome, which is associated with cerebellar hypoplasia. Previous in vitro studies demonstrated ethanol-impaired neuronal survival reduced signaling through the insulin receptor (IRβ). We examined an experimental rat model of chronic gestational pups exhibited striking hypoplasia increased apoptosis. Immunoprecipitation and Western blot analyses detected levels tyrosyl-phosphorylated IRβ, substrate-1 (IRS-1), p85-associated IRS-1 but no...
Familial Alzheimer's Disease (AD) has been linked to amyloid beta protein precursor (AbetaPP) and presenilin gene mutations. In sporadic AD, which accounts for the vast majority of cases, pathogenesis neurodegeneration is unknown; however, recent evidence suggests a role oxidative stress. The present study demonstrates that transient hypoxic injury cortical neurons causes several molecular biochemical abnormalities occur in AD including, mitochondrial dysfunction, impaired membrane...
Chronic ethanol consumption can cause sustained hepatocellular injury and inhibit the subsequent regenerative response. These effects of may be mediated by impaired hepatocyte survival mechanisms. The present study examines on signaling in intact liver. Adult Long Evans rats were maintained ethanol–containing or isocaloric control liquid diets for 8 weeks, after which livers harvested to measure mRNA levels, protein expression, kinase phosphatase activity related proapoptosis exposure...
Background Oxidative stress and mitochondrial dysfunction are central mediators of cardiac after ischemia/reperfusion. ATP binding cassette erythroid (ABC-me; ABCB10; mABC2) is a transporter highly induced during differentiation predominantly expressed in bone marrow, liver, heart. Until now, ABC-me function heart was unknown. Several lines evidence demonstrate that the yeast ortholog protects against increased oxidative stress. Therefore, potential modulator outcome ischemia/reperfusion...
Abstract Background Aspartyl (asparaginyl)-β-hydroxylase (AAH) hydroxylates Asp and Asn residues within EGF-like domains of Notch Jagged, which mediate cell motility differentiation. This study examines the expression, regulation function AAH, its related transcripts, Humbug Junctin, lack catalytic domains, using SH-Sy5y neuroblastoma cells. Results Real time quantitative RT-PCR demonstrated 8- or 9-fold higher levels than AAH lower all 3 transcripts in normal human brains compared with...
The interactions between Na+ (and K+) and Asp-201 of beta-galactosidase were studied. Analysis the changes in Km Vmax showed that Kd for wild type (0.36 +/- 0.09 mM) was about 10x lower than K+ (3.9 0.6 mM). difference is probably because size other physical properties ions binding pocket. Decreases as functions oNPG pNPG decreases Ki both shallow deep mode inhibitors similar, whereas substrates without C6 hydroxyls remained constant. Thus, are important galactosyl moieties via hydroxyl...
Abstract Alterations to the community structure and function of microbiome are associated with changes host physiology, including immune responses. However, contribution microbial-derived RNAs carried by outer membrane vesicles (OMVs) responses remains unclear. This paper investigates role OMVs OMV-associated small RNA (sRNA) species from pathogenic commensal Bacteroides fragilis (ETBF NTBF respectively) in eliciting different intestinal epithelial cells. To distinguish differences sRNA...