A5091577020- Wheat and Barley Genetics and Pathology
- Agricultural Productivity and Crop Improvement
- Genetic Mapping and Diversity in Plants and Animals
- Horticultural and Viticultural Research
- Agriculture and Biological Studies
- Polysaccharides Composition and Applications
- Genetic and Environmental Crop Studies
- Plant Genetic and Mutation Studies
- Genetic diversity and population structure
- Bioenergy crop production and management
- Plant Reproductive Biology
- Fermentation and Sensory Analysis
- Plant Disease Resistance and Genetics
- Polysaccharides and Plant Cell Walls
- Gene expression and cancer classification
- Genetics and Plant Breeding
- Biofuel production and bioconversion
- Botany and Plant Ecology Studies
- Plant tissue culture and regeneration
- Forest ecology and management
- Botanical Research and Chemistry
- Yeasts and Rust Fungi Studies
- Forest Management and Policy
- Crop Yield and Soil Fertility
- Lipid metabolism and biosynthesis
Skolkovo Institute of Science and Technology
2023-2024
Saint Petersburg State Forest Technical University
2019-2023
National Institute of Grape and Wine "Magarach"
2021-2022
Sirius University of Science and Technology
2021
N.I. Vavilov Research Institute of Plant Industry
2010-2020
Leibniz Institute of Plant Genetics and Crop Plant Research
2002-2019
St Petersburg University
2019
European University at Saint Petersburg
2018
Krasnodar Research Institute of Storage and Processing of Agricultural Products
2017
Lomonosov Moscow State University
2016
Transcript abundance from cRNA hybridizations to Affymetrix microarrays can be used for simultaneous marker development and genome-wide gene expression quantitative trait locus (eQTL) analysis of crops. We have previously shown that it is easily possible use arrays profile individuals a segregating population accurately identify robust polymorphic molecular genetic markers. applied the method more than 2000 polymorphisms (transcript derived markers, TDMs) an experiment involving two...
Summary To provide resources for barley genomics, 110 981 expressed sequence tags (ESTs) were generated from 22 cDNA libraries representing tissues at various developmental stages. This EST collection corresponds to approximately one‐third of the 380 000 publicly available ESTs. Clustering and assembly resulted in 14 151 tentative consensi (TCs) 11 073 singletons, altogether 25 224 putatively unique sequences. Of these, 17.5% showed no significant similarity other ESTs present dbEST. More...
Different aspects of androgenesis induction have been studied in detail, but little is known about the molecular mechanisms associated with this developmental switch. We employed macroarrays containing 1421 expressed sequence tags covering early stages barley zygotic embryogenesis to compare gene expression profiles stress‐induced androgenic microspores those uninucleate as they progressed into binucleate stage during pollen development. Principal component analysis defined distinct sets...
Abstract The recent development of Affymetrix chips designed from assembled EST sequences has spawned considerable interest in identifying single-feature polymorphisms (SFPs) transcriptome data. SFPs are valuable genetic markers that potentially offer a physical link to the structural genes themselves. However, most current SFP prediction methodologies were developed for sequenced species although particularly with complex and unsequenced genomes. To establish sensitivity specificity...
Restriction-site associated DNA sequencing (RADseq) technology was recently employed to identify a large number of single nucleotide polymorphisms (SNP) for linkage mapping North American and Eastern Asian Populus species. However, there is also the need high-density genetic maps European aspen (P. tremula) as tool further quantitative trait loci (QTLs) marker-assisted selection species native Europe.We established hybrid F1 population from cross two parental genotypes diverged in their...
Abstract Background Affymetrix high density oligonucleotide expression arrays are widely used across all fields of biological research for measuring genome-wide gene expression. An important step in processing microarray data is to produce a single value the level an RNA transcript using one growing number statistical methods. The challenge researcher decide on most appropriate method use address specific question with given dataset. Although several efforts have focused assessing...
Abstract Background A typical genetical genomics experiment results in four separate data sets; genotype, gene expression, higher-order phenotypic and metadata that describe the protocols, processing array platform. Used concert, these sets provide opportunity to perform genetic analysis at a systems level. Their predictive power is largely determined by expression dataset where tens of millions points can be generated using currently available mRNA profiling technologies. Such large,...
Abstract Background Guar ( Cyamopsis tetragonoloba (L.) Taub.), a short-day plant, is an economically valuable legume crop. Seeds of guar serve as source galactomannan polysaccharide, known gum, which in demand the gas and oil industries. The rapid complete maturation seeds depends on flowering time particular genotype. It that controlled by several gene systems. However, no information about process mechanisms trigger molecular biochemical levels was previously reported. aim study to...
Non-synonymous coding mutations in a gene change the resulting protein, no matter where it is expressed, but effects of cis-regulatory could be spatially or temporally limited - phenomenon termed pleiotropy. Here, we report genome-wide occurrence pleiotropy barley (Hordeum vulgare L.) using Affymetrix analysis 22,840 genes population 139 doubled haploid lines derived from cross between cultivars Steptoe (St) and Morex (Mx). We identified robust cis-acting expression regulators that segregate...
Photoperiod insensitive Ppd-1a alleles determine early flowering of wheat. Increased expression homoeologous Ppd-D1a and Ppd-A1a result from deletions in the promoter region, elevated Ppd-B1a is determined by an increased copy number.In this study, using bread wheat cultivars Sonora PSL2, which contrast time, near-isogenic lines resulting their cross, "Ppd-m" "Ppd-w" with introgressed Sonora, we investigated putative factors that influence expression. By analyzing three distinct copies,...
Alike to Reduced height-1 (Rht-1) genes in wheat and the semi dwarfing (sd-1) gene rice, sdw1/denso locus involved metabolism of GA, was designated as 'Green Revolution' barley. The recent molecular characterization candidate HvGA20ox2 for allows estimate impact functional polymorphism this on variation agronomically important traits
It is well known that Affymetrix microarrays are widely used to predict genome-wide gene expression and genetic polymorphisms from RNA genomic DNA hybridization experiments, respectively. has recently been proposed integrate the two predictions by use of microarray data only. Although ability detect single feature (SFPs) many practical implications for genome study in both sequenced unsequenced species, it raises enormous challenges statistical modelling analysis this objective. Several...
The Crimean autochthonous grape varieties are unique by their origin and serve as a valuable source for breeding new cultivars with increased salt frost resistance, well high-quality berries. However, they suffer from fungal pathogens, the dry hot summer months contribute to epiphytotic course of diseases. An increase in resistance is currently achieved through interspecific hybridization. In this study, we describe genetic agrobiological diversity three hybrid populations obtained using...
Abstract Curly (Karelian) birch is a special variety of Betula pendula Roth distributed in the northwestern part Europe. Karelian well-known for its valuable figured curly wood also known as “wooden marble”. The genetic basis underlying formation has been debated since last century, however, there was no data about loci responsible trait. In present study, we analyzed two full-sibs populations derived from experimental crosses birches and segregating RADseq genotyping applied to reveal how...