A5090053515- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- thermodynamics and calorimetric analyses
- Computational Drug Discovery Methods
- Biochemical effects in animals
- Molecular Biology Techniques and Applications
- Meat and Animal Product Quality
- Toxin Mechanisms and Immunotoxins
- Genetic Neurodegenerative Diseases
- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Protein Structure and Dynamics
- Spaceflight effects on biology
- Prion Diseases and Protein Misfolding
- Ubiquitin and proteasome pathways
- Bee Products Chemical Analysis
- Alzheimer's disease research and treatments
- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
- Cell death mechanisms and regulation
- ATP Synthase and ATPases Research
- Cardiomyopathy and Myosin Studies
- Viral gastroenteritis research and epidemiology
Institute of Cytology
2015-2024
Aristotle University of Thessaloniki
2021
Russian Academy of Sciences
2006-2020
Howard Hughes Medical Institute
2020
New York University
2020
Klinik für Frauenheilkunde
2020
München Klinik
2020
Klinikum rechts der Isar
2020
St Petersburg University
2020
Johannes Gutenberg University Mainz
2006
Abstract Plant-derived extracellular vesicles (EVs) gain more and attention as promising carriers of exogenous bioactive molecules to the human cells. Derived from various edible sources, these EVs are remarkably biocompatible, biodegradable highly abundant plants. In this work, grapefruit juice were isolated by differential centrifugation followed characterization their size, quantity morphology nanoparticle tracking analysis, dynamic light scattering, atomic force microscopy cryo-electron...
Abstract The release of Hsp70 chaperone from tumor cells is found to trigger the full-scale anti-cancer immune response. Such and proper reaction can be induced by delivery recombinant a we sought explore how endogenous transported extracellular space leading burst activity. transport mechanisms were studied analyzing its intracellular tracks with Rab proteins as well using specific inhibitors membrane domains. To study forms released employed assay consisting two affinity chromatography...
The key feature of polyglutamine aggregates accumulating in the course Huntington disease (HD) is their resistance to protein denaturants, and date only chaperones are proved prevent mutant aggregation. It was suggested that expanded chains (polyQ) huntingtin cross-linked other proteins such as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Here we clarify roles GAPDH molecular chaperone Hsp70 formation sodium dodecyl sulfate (SDS)-insoluble polyQ aggregates. First, addition pure found...
Macrophages constitute a major part of tumor microenvironment, and most existing data demonstrate their ruling role in the development anti-drug resistance cancer cell. One powerful protection system is based on heat shock proteins whose synthesis triggered by activated Heat Shock Factor-1 (HSF1); inhibition HSF1 with CL-43 sensitized A549 lung cells to anti-cancer effect etoposide. Notably, analyzing xenografts mice we observed nest-like pattern co-localization demonstrating enhanced...
Hsp70 chaperone is known to stimulate anti-tumour immunity in a variety of cancer models. Here we demonstrated that the addition purified recombinant culture medium facilitated cell cytolysis by lymphocytes. Importantly, exogenous triggered secretion intracellular surface and extracellular milieu, which played role because down-regulation endogenous reduced both its presence at lymphocyte-mediated cytolysis. Inhibitors target ATPase peptide-binding domains molecule potently decreased...
Neuronal cell death at late stages of Alzheimer’s disease (AD) causes the release cytosolic proteins. One most abundant such proteins, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), forms stable aggregates with extracellular amyloid-β (Aβ). We detect these in cerebrospinal fluid (CSF) from AD patients levels directly proportional to progressive AD. found that GAPDH a covalent bond Q15 Aβ is mediated by transglutaminase (tTG). The Q15A substitution weakens interaction between and reduces...
Abstract Hsp70 chaperone controls proteostasis and anti-stress responses in rapidly renewing cancer cells, making it an important target for therapeutic compounds. To date several inhibitors are presented with remarkable anticancer activity, however their clinical application is limited by the high toxicity towards normal cells. This study aimed to develop assays search substances that reduce activity of diminish its protective function On our mind resulting compounds alone should be safe...
Abstract: Intratumoral injections of recombinant heat shock protein (Hsp)70 were explored for feasibility in patients with brain tumors. Patients aged 4.5–14 years untreated newly diagnosed tumors (n=12) enrolled. After tumor resection, five Hsp70 (total 2.5 mg) administered into the resection cavity through a catheter. Before administration and after last injection, specific immune responses to autologous lysate evaluated using delayed-type hypersensitivity test. Further, peripheral blood...
Heat shock protein 70, (Hsp70) constitutes a powerful system of cytoprotection in all organisms studied to date. Exerting such activity, Hsp70 rescues cancer cells from antitumor therapy, posing great challenge for oncologists. In contrast its protective action, was found be released cells, prompting cytotoxic lymphocytes target and kill the tumor. A number vaccines have been developed on basis ability present tumor antigen or elevate sensitivity lymphocytes. this commentary, we consider...