A5003379510- Cancer Cells and Metastasis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- Colorectal Cancer Treatments and Studies
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Genetic factors in colorectal cancer
- Chromatin Remodeling and Cancer
- Epigenetics and DNA Methylation
- Cognitive and developmental aspects of mathematical skills
- Liver Diseases and Immunity
- Cancer, Lipids, and Metabolism
- 3D Printing in Biomedical Research
- Statistics Education and Methodologies
- Cancer-related gene regulation
- Cancer Research and Treatments
- Phagocytosis and Immune Regulation
- Mathematics Education and Teaching Techniques
- ATP Synthase and ATPases Research
- Pharmacogenetics and Drug Metabolism
- Protein Degradation and Inhibitors
- Hepatocellular Carcinoma Treatment and Prognosis
- Immunotherapy and Immune Responses
University of Wisconsin–Madison
2022-2024
Madison Group (United States)
2024
University of California, Irvine
2023
Utah State University
2023
University of Wisconsin Carbone Cancer Center
2023
548 Background: Cholangiocarcinoma (CCA) requires integrated efforts to understand the heterogeneity in available molecular targets. Chemotherapy remains a non-selective standard of care, however defining distinct populations with actionability critical need. The Veteran Health Administration’s (VHA) National Precision Oncology Program (NPOP) was established characterize comprehensive genomic profiles across VHA network. Methods: Veterans CCA underwent next-generation sequencing (NGS)...
546 Background: Cholangiocarcinoma (CCA) has poor prognosis and limited treatment options. The US Veteran Health Administration’s (VHA) National Precision Oncology Program (NPOP) was established to characterize pathogenic drivers across the integrated VA network. Here, we summarize an important subgroup of CCA with homologous recombination deficiency (HRD) its impact on overall survival (OS). Methods: Next-generation sequencing (NGS) performed using FoundationOne CDx Analysis included...
Abstract Background: Pancreatic ductal adenocarcinoma (PDAC) remains a leading cause of cancer related mortality in the United States, largely due to ineffective systemic therapy. While novel KRAS target strategies are development, few agents have been investigated transcriptional machinery for global inhibition expression. Recent small molecule inhibitors targeting transcription (i.e., CDK7) shown preliminary evidence disease control; however, combination therapies lagged, likely...
Abstract Background: Cholangiocarcinoma (CCA) remains a highly morbid cancer for which accurate models. Patient-derived organoid (PCOs) have been generated in CCA, however their utility clinical prediction has not validated. Z’ factoring often fails assessing CCA response. Prior analysis response shown an effect size by Glass’s Delta (GΔ) of ≥1.25 growth inhibition is predictive Here, we present subclonal across diverse set models to therapy and level viability Methods: PCOs were expanded...
Abstract Background: Pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer mortality with primary hallmark, including dense collagen matrix in the desmoplastic stroma. Organoid technologies often rely on commercially available EHS mouse sarcoma materials, and it remains uncertain how batch-to-batch variability this impacts consistency signaling. Here, we present novel photo-activated hydrogel tuned to properties stroma PDAC organoid cultures. Method: We designed multiple...
Pancreatic and ampullary cancers remain highly morbid diseases for which accurate clinical predictions are needed precise therapeutic predictions. Patient-derived cancer organoids have been widely adopted; however, prior work has focused on well-level sensitivity. To characterize individual oligoclonal units of response, we introduce a low-volume screening assay, including an automated alignment algorithm. The growth response was compared against validated markers viability single-cell...
Abstract Background: Cancer stem cells (CSCs) have been described to confer resistance and aggressive disease biology in pancreatic ductal adenocarcinoma (PDAC) ampullary carcinoma (AMPC). Patient derived cancer organoids (PCOs) are an important model of PDAC that maintain the molecular features advanced shown predict drug response. This study aimed determine dynamics CSC expression on oligoclonal culture growth three independent PCOs models. Methods: were assayed using low volume 10μL...
Abstract Background: Organotypic models have emerged as an important preclinical model for developmental therapeutics in pancreatic and biliary cancer. Naturally derived cardiac glycoside 2D monoculture exquisite potency, here we describe their activity cancer normal liver with patient-derived organoids (PCOs). Methods: PCOs of ampullary cancers hepatic tissues were generated. Response was assayed four structurally different (cerberin, neriifolin, digitoxin, digoxin; 72h). characterized by...
Abstract Background: Therapeutic screening Pancreatic Ductal Adenocarcinoma (PDAC) relies on well-level assessment for high throughput response evaluation. Patient-derived cancer organoids (PCOs) model subclonal populations, however the significance of resistant populations is uncertain when characterized with response. Using a high-throughput assay, we present an automated alignment algorithm to characterize organoid growth as compared validated therapeutic assays. Methods: High content...
Abstract Background Metastatic pancreas adenocarcinoma (PDAC) is highly lethal and minimally responsive to immunotherapy. Gamma delta T cells (gDTs) are a unique cell subset found within the PDAC microenvironment. Animal models have shown that gDTs promote oncogenic progression ablating gDT delays cancer improves survival. Understanding activity of immune effector against organoids provides scalable platform understand impact activation small molecule modulation redirect killing in PDAC....
Abstract Background: Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer mortality in United States (US) with a 10% 5-year survival. Acquired resistance plays critical role progression, however targeted therapeutics have shown minimal clinical activity. There remains an unmet need to provide combination therapies across breadth development. KinderMiner highly efficient text mining algorithm developed predict association search terms publicly available research....
The Fraction Ball has demonstrated positive impacts on rational number learning.fractions and decimals through basketball.We explored activity features that sparked math talk discussions.We found 6th graders engaged in minimal discussions were mostly preceded by teacher prompting.Future designs might leverage student prior knowledge incorporating increasingly difficult questions to support peer-led discussions.
Abstract Background: Therapeutic development in pancreatic ductal adenocarcinoma (PDAC) requires accurate models for assessing response. Patient-derived cancer organoids (PCOs) have been shown to faithfully recapitulate genomic features of advanced PDAC. Mechanisms subclonal resistance or intratumoral heterogeneity cannot be characterized using well-level viability assays. Methods: A high-content imaging platform was adapted low volume, 96-well format Cytation 5 (Biotek). PDAC PCOs were...