A5020507749- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Acne and Rosacea Treatments and Effects
- CRISPR and Genetic Engineering
- Microbial Fuel Cells and Bioremediation
- Algal biology and biofuel production
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Streptococcal Infections and Treatments
- Biocrusts and Microbial Ecology
- CAR-T cell therapy research
- Herpesvirus Infections and Treatments
- Biochemical and Structural Characterization
- Enzyme Structure and Function
- Maternal and fetal healthcare
- Amino Acid Enzymes and Metabolism
- Hemoglobin structure and function
- Uterine Myomas and Treatments
- Antimicrobial Peptides and Activities
- Virus-based gene therapy research
- Pharmacological Effects of Natural Compounds
Universidad de Sevilla
2018-2024
Instituto de Bioquímica Vegetal y Fotosíntesis
2018-2024
Rensselaer Polytechnic Institute
2022-2024
Federación Colombiana de Obstetricia y Ginecología
2024
Utrecht University
2022-2024
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2023
The San Raffaele Telethon Institute for Gene Therapy
2023
Istituti di Ricovero e Cura a Carattere Scientifico
2023
Uppsala University
2022
Consejo Superior de Investigaciones Científicas
2019-2021
In genome engineering, the integration of incoming DNA has been dependent on enzymes produced by dividing cells, which a bottleneck toward increasing insertion frequencies and accuracy. Recently, RNA-guided transposition with CRISPR-associated transposase (CAST) was reported as highly effective specific in Escherichia coli. Here, we developed Golden Gate vectors to test CAST filamentous cyanobacteria show that it is Anabaena sp. strain PCC 7120. The comparatively large plasmids containing...
ABSTRACT Microbial extracellular proteins and metabolites provide valuable information concerning how microbes adapt to changing environments. In cyanobacteria, dynamic acclimation strategies involve a variety of regulatory mechanisms, being ferric uptake regulator as key players in this process. the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120, FurC (PerR) is global that modulates peroxide response several genes involved photosynthesis nitrogen metabolism. To investigate...
We report the high susceptibility of several clinical isolates Propionibacterium acnes from different sources (skin, bone, wound exudates, abscess or blood contamination) to head-to-tail cyclized bacteriocin AS-48. This peptide is a feasible candidate for further pharmacological development against this bacterium, due its physicochemical and biological characteristics, even when it growing in biofilm. Thus, treatment pre-formed biofilms with AS-48 resulted dose- time-dependent disruption...
Cyanobacteria are unique among the eubacteria as they possess a hybrid Gram phenotype, having an outer membrane but also comparably thick peptidoglycan sheet. Furthermore, cyanobacterial divisome includes proteins specific for both types well cyanobacteria-specific proteins. Cells in multicellular cyanobacteria share continuous periplasm and their cytoplasms connected by septal junctions that enable communication between cells filament. The localization of junction depends on interaction...
Abstract The organismic unit of heterocyst-forming cyanobacteria is a filament communicating cells connected by septal junctions, proteinaceous structures bridging the cytoplasms contiguous cells. This distinct bacterial organization preserved during cell division. In Anabaena , deletion zipN gene could not be segregated. We generated strain CSL109 that expresses from synthetic regulatable promoter. Under conditions ZipN depletion, progressively enlarged, reflecting restricted division, and...
In filamentous heterocyst-forming (N2-fixing) cyanobacteria, septal junctions join adjacent cells, mediating intercellular communication, and are thought to traverse the peptidoglycan through nanopores. Fluorescence recovery after photobleaching (FRAP) analysis with fluorescent marker calcein showed that cultures of Anabaena sp. strain PCC 7120 grown in presence combined nitrogen contained a substantial fraction noncommunicating cells (58% 80% tested vegetative nitrate- ammonium-grown...
Quiescent human hematopoietic stem cells (HSC) are ideal targets for gene therapy applications due to their preserved stemness and repopulation capacities; however, they have not been exploited extensively because of resistance genetic manipulation. We report here the development a lentiviral transduction protocol that overcomes this in long-term repopulating quiescent HSC, allowing efficient Mechanistically, vector HSC was found be restricted at level entry by limited pyrimidine pools....
N2 -fixing heterocystous cyanobacteria grow as chains of cells that are connected by proteinaceous septal junctions, which traverse the peptidoglycan through nanopores and mediate intercellular molecular transfer. In model organism Anabaena sp. strain PCC 7120, proteins SepJ, FraC FraD, localized at cell poles in septa, needed to produce junctions. The pentapeptide-repeat, membrane-spanning protein HglK has been described be involved deposition heterocyst-specific glycolipid layer, but hglK...
Filamentous, heterocyst-forming cyanobacteria are multicellular organisms in which growth requires the activity of two interdependent cell types that exchange nutrients and regulators. Vegetative cells provide heterocysts with reduced carbon, vegetative fixed nitrogen. Additionally, heterocyst differentiation from is regulated by inhibitors produced prospective heterocysts. Proteinaceous structures known as septal junctions join filament. The SepJ protein involved formation model...
Cyanobacteria are photosynthetic organisms with a Gram-negative envelope structure. Certain filamentous species such as
The TonB-dependent transport of scarcely available substrates across the outer membrane is a conserved feature in Gram-negative bacteria. plasma membrane-embedded TonB-ExbB-ExbD accomplishes complex functions as an energy transducer by physically interacting with transporters (TBDTs). TonB mediates structural rearrangements substrate-loaded TBDTs that are required for substrate translocation into periplasm. In model heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, four...
Background Global reports show that the antimicrobial-resistance of Propionibacterium acnes isolated from patients with acne vulgaris is becoming a large problem, making it necessary to find new therapeutic drugs. Methods In this study, 23 clinical isolates P. have been identified by MaldiToff and specific PCR. The susceptibility theses strains antibiotics (clindamicin, erytromycin tetracicline) bacteriocin (AS-48) has established, using CECT 5684 strain as reference. Moreover, we...
CLÍNICO ResumenLa inversión uterina es una patología de presentación rara e infrecuente.Se presenta el caso paciente con no puerperal requerimiento histerectomía abdominal total.Sin antecedentes importancia asistió al servicio urgencias por un cuadro clínico dolor 10 días evolución asociado a sangrado vaginal abundante inestabilidad hemodinámica y sensación masa vaginal.Al examen físico se evidenció sobresaliente del canal aspecto necrótico, lo que sospechó mioma nascens.Durante la estancia...
Abstract Cyanobacteria are among the most morphologically diverse prokaryotic phyla on Earth. Their morphotypes range from unicellular to multicellular filaments, yet mechanisms underlying evolution of filamentous morphologies remain unknown. Here, we implement phylogenomic, Bayesian molecular clock and gene-tree-species-tree reconciliation analyses estimate when genes encoding cell-cell joining structures first evolved. We also characterise septal measure intercellular communication rates...
Multicellular heterocyst-forming cyanobacteria, such as Anabaena, grow chains of cells forming filaments that, under diazotrophic conditions, contain two cell types: vegetative that perform oxygenic photosynthesis and N2-fixing heterocysts. Along the filament, intercellular septa a thick peptidoglycan layer forms septal disks. Proteinaceous junctions connect in filament traversing disks through nanopores. The fraCDE operon encodes proteins needed to make long Anabaena. FraC FraD, located at...
Abstract In genome engineering, integration of incoming DNA has been dependent on enzymes produced by dividing cells which a bottle neck towards increasing DNA-insertion frequencies and accuracy. Recently, RNA-guided transposition with CRISPR-associated transposase (CAST) was reported as highly effective specific in Escherichia coli . Here we developed Golden-Gate vectors to test CAST filamentous cyanobacteria show that it is Anabaena sp. strain PCC 7120. The comparatively large plasmids...
Background Global reports show that the antimicrobial-resistance of Propionibacterium acnes isolated from patients with acne vulgaris is becoming a large problem, making it necessary to find new therapeutic drugs. Methods In this study, 23 clinical isolates P. have been identified by MaldiToff and specific PCR. The susceptibility theses strains antibiotics (clindamicin, erytromycin tetracicline) bacteriocin (AS-48) has established, using CECT 5684 strain as reference. Moreover, we...