A5044956761- Metalloenzymes and iron-sulfur proteins
- Microbial Community Ecology and Physiology
- Metal Extraction and Bioleaching
- Bacterial Infections and Vaccines
- Photosynthetic Processes and Mechanisms
- Pneumonia and Respiratory Infections
- Microbial Fuel Cells and Bioremediation
- Electrocatalysts for Energy Conversion
- Porphyrin Metabolism and Disorders
- Fungal Infections and Studies
- Legume Nitrogen Fixing Symbiosis
- Metal-Catalyzed Oxygenation Mechanisms
- Advanced battery technologies research
- Antifungal resistance and susceptibility
- Nitrogen and Sulfur Effects on Brassica
- Biochemical and Molecular Research
- Redox biology and oxidative stress
- Cassava research and cyanide
- Enzyme Production and Characterization
- Corrosion Behavior and Inhibition
- Enzyme Structure and Function
- Mine drainage and remediation techniques
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
The University of Queensland
2016-2025
AgriBio
2017-2024
ASM International
2021
In-Q-Tel
2013
University of Oxford
2010
University of Arizona
2003-2009
University of Saskatchewan
2006
Daresbury Laboratory
2006
Institut für Medizinische Biometrie, Informatik und Epidemiologie
2000-2001
University of Bonn
1998
The Ralstonia solanacearum species complex has long been recognized as a group of phenotypically diverse strains that can be subdivided into four phylotypes. Using polyphasic taxonomic approach on an extensive set strains, this study provides evidence for and nomenclatural revision members complex. Data obtained from phylogenetic analysis 16S-23S rRNA ITS gene sequences, intergenic spacer (ITS) region sequences partial endoglucanase (egl) DNA-DNA hybridizations demonstrate the R. comprises...
Short-chain fatty acids are known modulators of host-microbe interactions and can affect human health, inflammation, outcomes microbial infections. Acetate is the most abundant but least well-studied these modulators, with studies focusing on propionate butyrate, which considered to be more potent. In this mini-review, we summarize current knowledge acetate as an important anti-inflammatory modulator between hosts microorganisms. This includes a summary pathways by metabolized bacteria...
Direct oxidation of sulfite to sulfate occurs in various photo- and chemotrophic sulfur oxidizing microorganisms as the final step reduced compounds is catalyzed by sulfite:cytochrome c oxidoreductase (EC ). Here we show that enzyme from Thiobacillus novellus a periplasmically located alphabeta heterodimer, consisting 40.6-kDa subunit containing molybdenum cofactor an 8.8-kDa mono-heme cytochrome c(552) (midpoint redox potential, E(m8.0) = +280 mV). The organic component was identified...
Sulfite-oxidizing molybdoenzymes convert the highly reactive and therefore toxic sulfite to sulfate have been identified in insects, animals, plants, bacteria. Although well studied enzymes from higher animals serve detoxify that arises catabolism of sulfur-containing amino acids, bacterial a central role converting formed during dissimilatory oxidation reduced sulfur compounds. Here we describe structure Starkeya novella dehydrogenase, heterodimeric complex catalytic molybdopterin subunit...
Sulfite dehydrogenase from Starkeya novella is an αβ heterodimer comprising a 40.6 kDa subunit (containing the Mo cofactor) and smaller 8.8 heme c subunit. The enzyme catalyses oxidation of sulfite to sulfate with natural electron acceptor being cytochrome c550. Its catalytic mechanism thought resemble that found in eukaryotic oxidases. Using protein film voltammetry redox potentiometry, we have identified both Mo- heme-centered responses immobilized on pyrolytic graphite working electrode:...
This paper describes a highly sensitive electrochemical (voltammetric) determination of sulfite using combination Starkeya novella dehydrogenase (SDH), horse heart cytochrome c (cyt c), and self-assembled monolayer 11-mercaptoundecanol (MU) cast on gold electrode. The biosensor was optimized in terms pH the ratio cyt c/SDH. electrocatalytic oxidation current increased linearly from 1 to 6 μM at enzyme-modified electrode with correlation coefficient 0.9995 an apparent Michaelis constant...
We have investigated the potential of GTP synthesis pathways as chemotherapeutic targets in human pathogen Cryptococcus neoformans, a common cause fatal fungal meningoencephalitis. find that de novo biosynthesis, but not alternate salvage pathway, is critical to cryptococcal dissemination and survival vivo. Loss inosine monophosphate dehydrogenase (IMPDH) pathway results slow growth virulence factor defects, while loss cognate phosphoribosyltransferase yielded no phenotypes. Further, species...
The arsenite oxidase (Aio) from the facultative autotrophic Alphaproteobacterium Rhizobium sp. NT-26 is a bioenergetic enzyme involved in oxidation of to arsenate. distantly related heterotroph, Alcaligenes faecalis, which thought oxidise for detoxification, consists large α subunit (AioA) with bis-molybdopterin guanine dinucleotide at its active site and 3Fe-4S cluster, small β (AioB) contains Rieske 2Fe-2S cluster. successful heterologous expression Aio Escherichia coli has resulted...
Haemophilus influenzae is a host adapted human pathogen known to contribute variety of acute and chronic diseases the upper lower respiratory tract as well middle ear. At sites infection during growth commensal environmental conditions encountered by H. will vary significantly, especially in terms oxygen availability, however, mechanisms which bacteria can adapt their metabolism cope with such changes have not been studied detail. Using targeted metabolomics spectrum metabolites produced on...
ABSTRACT Sulfoxide reductases in pathogenic bacteria have recently received increasing attention for their association with virulence and survival within the host. Here, we re-investigated physiological role of molybdenum-containing DmsABC dimethyl sulfoxide (DMSO) reductase from Escherichia coli , which has a proposed anaerobic respiration DMSO. Our investigation into potential substrates revealed that efficiently reduces pyrimidine N-oxide, nicotinamide methionine sulfoxide, exposure to...
ABSTRACT NtrYX is a sensor-histidine kinase/response regulator two-component system that has had limited characterization in small number of Alphaproteobacteria . Phylogenetic analysis the response NtrX showed this extensively distributed across bacterial domain, and it present variety Betaproteobacteria , including human pathogen Neisseria gonorrhoeae Microarray revealed expression several components respiratory chain was reduced an N. ntrX mutant compared to isogenic wild-type (WT) strain...
Metallosphaera sedula is a thermoacidophilic Crenarchaeon which capable of leaching metals from sulfidic ores. The authors have investigated the presence and expression genes encoding respiratory complexes in this organism when grown heterotrophically or chemolithotrophically on either sulfur pyrite. three gene clusters, two terminal oxidase complexes, quinol SoxABCD SoxM supercomplex, cluster high-potential cytochrome b components bc(1) complex analogue (cbsBA-soxL2N cluster) was...
Interprotein electron transfer underpins the essential processes of life and relies on formation specific, yet transient protein-protein interactions. In biological systems, detoxification sulfite is catalyzed by sulfite-oxidizing enzymes (SOEs), which interact with an acceptor for catalytic turnover. Here, we report structural functional analyses SOE SorT from Sinorhizobium meliloti its cognate SorU. Kinetic thermodynamic SorT/SorU interaction show complex dynamic in solution, that proteins...
H. influenzae is a host adapted human mucosal pathogen involved in variety of acute and chronic respiratory tract infections, including Chronic Obstructive Pulmonary Disease (COPD) asthma, all which rely on ability to efficiently establish continuing interactions with the host. Here we report characterization novel molybdenum enzyme, TorZ/MtsZ that supports cells during growth oxygen-limited environments. Strains lacking showed reduced survive contact epithelial as shown by...