A5050207161- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical sensors and biosensors
- Biodiesel Production and Applications
- Click Chemistry and Applications
- RNA Research and Splicing
- Ionic liquids properties and applications
- Genetic and Kidney Cyst Diseases
- Biofuel production and bioconversion
- Polymer Surface Interaction Studies
- biodegradable polymer synthesis and properties
- MicroRNA in disease regulation
- Growth Hormone and Insulin-like Growth Factors
- Endoplasmic Reticulum Stress and Disease
- Birth, Development, and Health
- Biotin and Related Studies
- Biomedical Research and Pathophysiology
- Porphyrin and Phthalocyanine Chemistry
- Photochromic and Fluorescence Chemistry
- Protein Interaction Studies and Fluorescence Analysis
- Cancer-related molecular mechanisms research
- Aquatic Ecosystems and Biodiversity
- Silk-based biomaterials and applications
- Analytical Chemistry and Chromatography
- Lubricants and Their Additives
Xinxiang University
2023
South China Agricultural University
2023
Bridge University
2023
Huazhong University of Science and Technology
2016-2022
Biodiesel production using immobilized lipase as a biocatalyst is promising process. The performance of mainly determined by supporting materials and immobilization method. To avoid the shortcomings adsorption covalent bonding methods, in this study, we developed novel heterofunctional carrier being strengthened anion exchange weakened binding to activity loss improve operational stability lipase. 2,3-epoxypropyltrimethylammonium chloride with epoxy quaternary ammonium group glutaraldehyde...
Microcystins (MCs) produced by cyanobacteria pose serious threats to human health. However, the contribution of long-term exposure MCs development nonalcoholic fatty liver disease (NAFLD) remains poorly documented. In this study, we estimated environmental uptake a small population fishers who have lived for many years on Meiliang Bay Lake Taihu, where cyanobacterial blooms occur frequently. Serum biochemical indices function and their relationships with MC contamination in these people were...
In East Asia, for thousands of years, the fruit Chinese tallow tree (Sapium sebiferum) has been used multiple purposes because its chemical composition; presence high amounts lipids is remarkable, showing potential to be as substrate biodiesel synthesis. Previously have reported use alkaline and enzymatic catalysts, microwave technology ionic liquids co-solvents with this species produce biodiesel. This study shows results Burkholderia cepacia lipase catalyst transesterification Tallow...
In order to address the challenges of relative ratio and position among individual enzymes substrate channeling in multienzyme biocatalysis, we propose a new one-pot genetically encoded self-assembly system as an alternative heavy procedure separately preparing subsequently co-immobilizing them by physicochemical means, allowing us construct cell-associated two- or three-enzyme complexes, through highly specific interaction cohesin dockerin domains. Our method for complexes (MECs) assembled...
With the rapid development of biotechnological industry, there is an urgent need for exploiting new materials to immobilize enzymes improve performance biocatalysts. In this paper, hierarchically porous magnetic microspheres (PFMMs) were prepared through solvothermal method and rapidly grafted with a novel rigid–flexible dendrimer first synthesized from monomers trimesoyl chloride (TMC) 1,6-hexanediamine (HDA) via interfacial polymerization process covalent immobilization Pseudomonas...
In this study, we overcame the limitations of single-enzyme system catalysis by codisplaying Candida rugosa lipase 1 (CRL1) and Rhizopus oryzae (ROL) on cell surfaces whole-cell catalyst Pichia pastoris to produce biodiesel from tallow seed oil. We screened double antibiotic-resistant strains tributyrin plates, performed second electroporation based single-displayed ROL GS115/KpRS recombinants CRL1 GS115/ZCS obtained an ROL/CRL1 codisplay P. GS115 surfaces. The maximum activity GS115/pRCS...
In order to achieve the industrialization of lipase-catalyzed biodiesel production, economic and effective carriers for immobilized lipase need be exploited. this study, Rhizomucor miehei (RML) was on different kinds macroporous resins NKA found best carrier. The RML then employed catalyze production from soybean oil. 8 organic solvents were used as media in trans-esterification reactions. Isooctane proved optimal one. Subsequently, immobilization conditions reaction further optimized...
The strong adhesion abilities of mussel adhesive protein in wet environment rely on complicated solidified processes. Among these processes, several are found to be key factors affecting underwater interfacial adhesion, including post-translational modification for tyrosine catalyzed by tyrosinase gland cells, storage and DOPA antioxidation vesicles after the reaction, delayed oxidation at interface pH change secretion seawater. In view this, an vitro system simulating natural process is...
Abstract To investigate the proliferation regulation role of 17β-Estradiol(E2)-induced miR-16-5p in mouse thymic epithelial cells. The miRNA expression profiles 50 nmo/L E2 treated MTEC1 cells were determined by high-throughput sequencing. Then significantly upregulated that responsive to was screened and validated. Subsequently, functions mechanism analyzed MiR-16-5p found had “high” levels among 36 upregulation miRNAs, which induced nmol/L E2. Transfection assays showed overexpression...
Abstract Background Estrogen signaling and microRNA (miRNA) expression play a pivotal role in thymus involution by regulating thymic epithelial cell (TECs) functions. However, the of on miRNA TECs has not been fully elucidated. Methods Cell proliferation assays, such as cell-counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU) flow cytometry assays were conducted. Next-generation sequencing (NGS)-based profiling was performed validated using Quantitative polymerase chain reaction (qPCR)....