A5006604356- Angiogenesis and VEGF in Cancer
- Supramolecular Self-Assembly in Materials
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
- Electrospun Nanofibers in Biomedical Applications
- Advanced biosensing and bioanalysis techniques
- 3D Printing in Biomedical Research
- Protease and Inhibitor Mechanisms
- Cholesterol and Lipid Metabolism
- Mesenchymal stem cell research
- Click Chemistry and Applications
- Nanoparticles: synthesis and applications
- Pulmonary Hypertension Research and Treatments
- Machine Learning in Bioinformatics
- Atherosclerosis and Cardiovascular Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Extracellular vesicles in disease
- Periodontal Regeneration and Treatments
- biodegradable polymer synthesis and properties
- Proteoglycans and glycosaminoglycans research
- Lipoproteins and Cardiovascular Health
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Heme Oxygenase-1 and Carbon Monoxide
- Adipokines, Inflammation, and Metabolic Diseases
- Cellular Mechanics and Interactions
University of North Carolina at Chapel Hill
2018-2021
Petersfield Community Hospital
2018
University of Colorado Boulder
2017-2018
Duke University
2012-2015
Noncompressible torso hemorrhage accounts for a significant portion of preventable trauma deaths. We report here on the development injectable, targeted supramolecular nanotherapeutics based peptide amphiphile (PA) molecules that are designed to target tissue factor (TF) and, therefore, selectively localize sites injury slow hemorrhage. Eight TF-targeting sequences were identified, synthesized into PA molecules, coassembled with nontargeted backbone at various weight percentages, and...
The rising prevalence of cardiovascular disease worldwide necessitates novel therapeutic approaches to manage atherosclerosis. Intravenously administered nanostructures are a promising noninvasive approach deliver therapeutics that reduce plaque burden. drug liver X receptor agonist GW3965 (LXR) can atherosclerosis by promoting cholesterol efflux from but causes toxicity when systemically at effective doses, thus preventing its clinical use. ability peptide amphiphile nanofibers containing...
Nanomedicine is a promising, noninvasive approach to reduce atherosclerotic plaque burden. However, drug delivery limited without the ability of nanocarriers sense and respond diseased microenvironment. In this study, nanomaterials are developed from peptide amphiphiles (PAs) that increased levels matrix metalloproteinases 2 9 (MMP2/9) or reactive oxygen species (ROS) found within niche. A pro-resolving therapeutic, Ac2-26, derived annexin-A1 protein, tethered PAs using linkages cleave in...
Co-assembled peptide amphiphile nanofibers designed to target atherosclerotic plaque and enhance cholesterol efflux are shown encapsulate deliver a liver X receptor agonist increase from murine macrophages in vitro. Fluorescence microscopy reveals that the nanofibers, which display an apolipoprotein-mimetic peptide, localize at sites LDL knockout mice with or without encapsulated molecule, while displaying scrambled, non-targeting sequence do not demonstrate comparable binding. These results...
Cardiovascular disease is the leading cause of death and disability in world. Atherosclerosis, buildup fatty deposits arteries, a major underlying cause. Nanomedicine an emerging treatment option to manage atherosclerotic plaque burden. Nanomaterials are critical success nanomedicine therapies through their ability enable targeted, controlled drug release. However, nanocarriers must be designed ensure that nanomaterials therapeutics work tandem, tailored respond unique physiochemical...
In this study, we tested the hypotheses that endothelial cells (ECs) derived from human umbilical cord blood (hCB-ECs) exhibit low permeability, which increases as hCB-ECs age and undergo senescence, change in permeability of is due to changes tight junction protein localization activity exchange activated by cAMP (Epac)1. Albumin across low-passage hCB-EC monolayers on Transwell membranes was 10 times lower than for aortic ECs (HAECs) ( P < 0.01) but similar vivo values arteries....
Small-diameter expanded polytetrafluoroethylene (ePTFE) graft surfaces have poor long-term patency due to limited endothelial cell (EC) coverage and anastomotic intimal hyperplasia. Multifunctional elastomers that coat the ePTFE surface promote EC adhesion while simultaneously inhibiting hyperplasia are highly desirable. Poly(diol-co-citrate) (PDC), a thermoset elastomer, is biodegradable, biocompatible, mimics vascular mechanical properties. Engineering antioxidant components into PDC...
Pulmonary hypertension is a highly morbid disease with no cure. Available treatments are limited by systemic adverse effects due to non-specific biodistribution. Self-assembled peptide amphiphile (PA) nanofibers biocompatible nanomaterials that can be modified recognize specific biological markers provide targeted drug delivery and reduce off-target toxicity. Here, PA target the angiotensin I-converting enzyme receptor for advanced glycation end-products (RAGE) developed, as both proteins...
Mesenchymal stem cells (MSCs) and endothelial progenitor (EPCs) represent promising cell sources for angiogenic therapies. There are, however, conflicting reports regarding the ability of MSCs to support network formation cells. The goal this study was assess human bone marrow-derived outgrowth (EOCs) derived from umbilical cord blood EPCs. We hypothesized that upon in vitro coculture, EOCs promote a microenvironment conducive EOC without addition growth supplements. networks formed by...
Coculture of endothelial cells (ECs) and smooth muscle (SMCs) in vitro can yield confluent monolayers or EC networks. Factors influencing this transition are not known. In study, we examined whether the spatial arrangement EC-SMC cocultures affected migration, network morphology, angiogenic protein secretion. Human umbilical cord blood-derived ECs (hCB-ECs) were grown coculture with human aortic SMCs either a mixed lamellar geometry analyzed over culture period 12 days. The hCB-ECs cultured...
The recently developed synthetic oligonucleotides referred to as "click" nucleic acids (CNAs) are promising due their relatively simple synthesis based on thiol–X reactions with numerous potential applications in biotechnology, biodetection, gene silencing, and drug delivery. Here, the cytocompatibility cellular uptake of rhodamine tagged, PEGylated CNA copolymers (PEG-CNA-RHO) were evaluated. NIH 3T3 fibroblast cells treated for 1 h 1, 10, or 100 μg/mL PEG-CNA-RHO maintained an average cell...
Abstract Atherosclerotic plaque remains the leading contributor to cardiovascular disease and requires invasive surgical procedures for its removal. Nanomedicine offers a minimally approach alleviate burden by targeted therapeutic delivery. However, nanocarriers are limited without ability sense respond diseased microenvironment. In this study, self‐assembled peptide amphiphile (PA) nanofibers developed that cleave in response biochemical cues expressed atherosclerotic lesions—reactive...
Front Cover: Plaque-targeting peptides and liver X receptor agonists self assemble to form amphipathic nanofibers. Upon intravenous injection, the nanofibers target atherosclerotic lesions reduced plaque burden. These findings demonstrate potential of supramolecular system applications in cardiovascular nanomedicine. This is reported by Neel A. Mansukhani, Erica B. Peters, Miranda M. So, Mazen S. Albaghdadi, Zheng Wang, Mark R. Karver, Tristan D. Clemons, Jeffrey P. Laux, Nick Tsihlis,...
ADVERTISEMENT RETURN TO ISSUEEditorialNEXTSpecial Issue: Nanomedicine Advances in Infectious DiseaseErica B. PetersErica PetersMore by Erica Petershttp://orcid.org/0000-0002-2669-5280 and Rinti BanerjeeRinti BanerjeeMore BanerjeeCite this: ACS Biomater. Sci. Eng. 2021, 7, 5, 1722–1724Publication Date (Web):May 10, 2021Publication History Received19 April 2021Published online10 May inissue 10...
Objective: Atherosclerosis is the leading cause of death and disability in United States. We hypothesize that systemic administration a novel nanofiber will target areas atherosclerosis regress atherosclerotic lesions. Methods: Self assembling peptide amphiphile (PA) nanofibers were synthesized. An 18 amino acid sequence which retains cholesterol efflux actions apolipoprotein-A1 (apoA1) along with Liver X Receptor (LXR) agonist, known to enhance efflux, incorporated into both treat...