A5013461053- Genetically Modified Organisms Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Insect Resistance and Genetics
- Identification and Quantification in Food
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Food Supply Chain Traceability
- Intellectual Property and Patents
- Animal Disease Management and Epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Legume Nitrogen Fixing Symbiosis
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Disease Resistance and Genetics
- Bacillus and Francisella bacterial research
- Plant Genetic and Mutation Studies
- Gene expression and cancer classification
- Emergency and Acute Care Studies
- Chemistry and Chemical Engineering
- Protein purification and stability
- Scientific Measurement and Uncertainty Evaluation
- Microbial Metabolism and Applications
Sciensano (Belgium)
2019-2024
Institut Scientifique de Santé Publique
2012-2017
Ghent University
2017
Ghent University Hospital
2017
Institute of Public Health Zadar
2012
Research Institute for Fisheries and Aquaculture
2010
Instituut voor Landbouw en Visserijonderzoek
2008
Agrobioinstitute
2001-2003
Standard data analysis pipelines for digital PCR estimate the concentration of a target nucleic acid by digitizing end-point fluorescence parallel micro-PCR reactions, using an automated hard threshold. While it is known that misclassification has major impact on and substantially reduces accuracy, uncertainty this classification typically ignored. We introduce model-based clustering method to probability present (absent) in partition conditional its observed distributional shape no-template...
Genetically modified microorganisms (GMM), harbouring commonly antimicrobial resistance (AMR) genes as selection markers, are frequently used to produce food and feed enzymes, additives flavourings. Such commercialized microbial fermentation products should not contain GMM, or associated recombinant DNA. Although the use of AMR gives rise public health environmental concerns regarding their potential acquisitions by pathogens gut microbiota, no method targeting harboured such GMM is...
Recently, we developed a DNA walking system for the detection and characterization of broad spectrum GMOs in routine analysis food/feed matrices. Here, present new version with improved throughput sensitivity by coupling to Pacific Bioscience® Next-generation sequencing technology. The performance strategy was thoroughly assessed through several assays. First, tested its identification capability on grains high or low GMO content. Second, potential impacts food processing were investigated...
As genetically modified microorganisms (GMM), commonly used by the food and feed industry to produce additives, enzymes flavourings, are frequently harbouring antimicrobial resistance (AMR) genes as selection markers, health environmental concerns were consequently raised. For this reason, interest of competent authorities control such microbial fermentation products has strongly increased, especially since several recent accidental contaminations unauthorized GMM, or associated recombinant...
Gene-edited organisms and derived food feed products commercialized on the European market falls within scope of Directive, 2001/18/EC. Therefore, possibility to specifically detect quantify them has become a priority. To this end, PCR-based approaches, such as real-time PCR digital droplet PCR, targeting single variation point carried by gene-edited organism are expected be suitable, even if potentially challenging at technical level. However, additional issues related interpretation...
GMO quantification, based on real-time PCR, relies the amplification of an event-specific transgene assay and a species-specific reference assay. The uniformity nucleotide sequences targeted by both assays across various transgenic varieties is important prerequisite for correct quantification. Single polymorphisms (SNPs) frequently occur in maize genome might lead to variation regions used design primers probes assays. Further, they may affect annealing primer template reduce efficiency DNA...
Recently, unexpected contaminations of unauthorized genetically modified microorganisms (GMM) carrying antimicrobial resistance (AMR) genes were reported in microbial fermentation products commercialized on the food and feed chain. To guarantee traceability safety chain, whole-genome sequencing (WGS) has played a key role to prove GMM via characterization unnatural associations sequences. However, WGS requires prior isolation strain, which can be difficult successfully achieve. Therefore,...
The increasing number and diversity of genetically modified organisms (GMOs) for the food feed market calls development advanced methods their detection identification. This issue can be addressed by next generation sequencing (NGS). However, efficiency NGS-based strategies depends on availability bioinformatic to find sequences transgenic insert junction regions, which is a challenging topic. To facilitate this task, we have developed Nexplorer, sequence-based database in annotated GM...
The last decade, smoke and smokeless products claiming to be tobacco-free, including herbal cigarettes shisha, became available on the European market gained popularity. This study proposes a new digital droplet PCR (ddPCR) method, designed based previously developed real-time (qPCR) method being currently used by U.S. Food Drug Administration (FDA) specifically detect presence of tobacco DNA in targeting sequence from Nicotiana tabacum nia-1 gene. To ensure harmonized reliable control...
Abstract Recently, the unexpected presence of a viable unauthorized genetically modified bacterium in commercialized food enzyme (protease) product originating from microbial fermentation process has been notified at European level (RASFF 2019.3332). This finding was made possible thanks to use next-generation sequencing technology, as reported this study. Whole-genome used characterize genetic modification comprising sequence pUB110 shuttle vector (GenBank: M19465.1), harbouring...
Abstract Unexpected contaminations of unauthorized genetically modified microorganisms (GMM) harbouring antimicrobial resistance (AMR) genes in food and feed enzymes, additives flavourings commercialized on the European market have recently alerted competent authorities regarding safety. At control level, we therefore proposed a PCR-based strategy as first line screening targeting GMM carrying AMR order to help enforcement laboratories. The potential presence frequently used is investigated,...
Abstract In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by walking anchored key targets, earlier detected real-time PCR screening analysis, be subsequently sequenced using MinION platform Oxford Nanopore Technologies. This way, presence was demonstrated via characterization their transgene flanking regions as...
A PCR-based DNA walking analysis was performed on a protease product suspected to contain new unauthorized genetically modified microorganism (GMM). Though the characterization of unnatural associations sequences between pUB110 shuttle vector and Bacillus amyloliquefaciens gene coding for protease, presence GMM shown. Based these interest, real-time PCR marker developed target specifically newly discovered GMM, namely protease2. The performance assessed in terms specificity sensitivity....
Abstract Using a recently developed genetically modified microorganisms (GMM) detection strategy, unexpected contaminations of unauthorized GMM in commercialized microbial fermentation products have been reported. A first-line real-time PCR screening analysis was initially performed to determine the presence key targets frequently found (GM) bacteria. second-line subsequently applied identify specific GMM, including date GM Bacillus velezensis producing protease and B. subtilis vitamin B 2 ....
The presence of a genetically modified microorganism (GMM) or its DNA, often harboring antimicrobial resistance (AMR) genes, in microbial fermentation products on the market is prohibited by European regulations. GMMs are currently screened for through qPCR assays targeting AMR genes and vectors, then confirmed known specific GM constructs/events. However, when GMM was not previously characterized an isolate cannot be obtained, proven. We present metagenomics approach capable delivering...
Recently, several methods were developed to detect genetically modified (GM) bacteria, allowing screen for the potential presence of bacteria and prove bacterial GM contaminations in fermentation products (such as FE). To obtain a first overview possible contamination FE with general workflow was proposed, combining recently detection methods. Those include 16S screening viability assessment target contaminations, well line second qPCR targeting GMM preparations. Subsequently, proposed...