The aim of our study was to examine the effects crocin (0.5 (C0.5), 1 (C1) and 1.5 (C1.5) mM) naringenin (50 (N50), 100 (N100) 150 (N150) μM) in cryopreservation extender for freezing rooster semen. Sperm motility, viability, abnormalities, membrane functionality, active mitochondria, apoptosis status, lipid peroxidation (LP), GPX, SOD, TAC, mRNA expression pro-apoptotic (CASPASE 3) anti-apoptotic (Bcl-2) genes, fertile eggs, hatched eggs hatching rate were investigated following...

Oxidative stress impairs sperm function and fertility in rams, most notably during cryopreservation, is characterized by compromised motility. Mitochondria contribute immensely towards function, such as mitochondrial membrane potential (MMP) which crucial for ATP production. The purpose of this study was to assess the role MitoPBN supplementation on cryopreserved quality mature Ghezel ram sperm. Semen samples were treated with 0 (control), 100, 150, 200, 250 µmol/L then analyzed key factors....

Liposomes could improve the delivery of substances to sperm. This study was conducted investigate effect antioxidant ellagic acid and acid-loaded liposomes on post-thawed sperm quality in broiler breeder roosters. Semen diluted Beltsville extender containing or (ellagic at 0 (control), 0.5, 1, 2 mM) cryopreserved. Sperm evaluated post-thawing: motility characteristics (Computer-Assisted Analysis), membrane functionality (HOS test), abnormal morphology, mitochondrial activity (Rhodamine 123),...

The present study focuses on the effect of different levels ergothioneine and thawing temperature rooster semen cryopreservation. Semen was diluted in Lake extender containing at 5, 10, 15, 20 µM cryopreserved. Two temperatures (37°C for 30 s 60°C 5 s) were consequently examined. Sperm motility parameter, membrane integrity, abnormal morphology, viability, apoptotic status, mitochondria activity, lipid peroxidation determined after freeze-thaw process. Ergothioneine 10 led to higher (P <...

Antifreeze proteins (AFP) have the potential for improving sperm cryopreservation. We applied Type III antifreeze protein (AFP3) on cryopreservation of spermatozoa from broiler breeder roosters, aiming to enhance post-thawing quality and fertility. Semen was extended at 37°C in Lake's extender containing AFP3 0.01, 0.1, 1, 5, 10 µg/mL (no as control). Post-thawing assessment included motility (CASA), morphology, membrane functionality by hypoosmotic swelling test (HOST), lipoperoxidation...

Glycerol is the most widely used cryoprotectant for rooster sperm because it declines mechanical damage to during freezing process. Despite its high molecular weight and viscosity, which may be cytotoxic, glycerol can cause cells cryopreservation process, resulting in less fertility. Poloxamer 188 (P188) an embryo supplement effective many species also cell lines plant cells. We tested suitability of P188 sperm, considering post-thawing motility, abnormalities, membrane functionality...

The purpose of this study was to investigate the effects rosiglitazone on ram semen after cryopreservation quality thawed sperm. Sperm motility, membrane functionality, viability, total abnormality, acrosome integrity, mitochondrial activity, reactive oxygen species production, ATP content and apoptotic features were assessed thawing. Rosiglitazone at concentration 60 µM resulted in highest (P < 0.05) progressive motility straight-line velocity. percentages average path velocity curvilinear...

The aim of the present study was to investigate effects tempol and straw size on rooster sperm post-thaw quality fertility. Rooster semen cryopreserved in Lake extender containing 0 (control), 5, 10, 15 20 μM (in two different size, 0.25 0.5). percentage total progressive motility, VAP VSL increased 10 µM group. Moreover, led lower ROS compared other groups. lowest apoptotic-like changes detected when treated with tempol. minimum observed group 0.5 size. Straw did not have any significantly...

Oxidative stress is associated with impaired sperm quality after thawing. Since mitochondria are the main source of reactive oxygen species (ROS) in sperm, aim this study was to investigate effects targeted mitochondrial antioxidant mitoquinone (MitoQ) and non-targeted pentoxifylline (PTX) during cooling cryopreservation rooster sperm. Sperm samples were collected from 15 roosters aged 28 wk diluted Beltsville extender. After dilution addition treatments (50, 100, 200 pMol MitoQ 0.5, 0.75, 1...

Antioxidant nanoparticles include the potential for improving sperm cryopreservation. The aim of performing this study was to evaluate effects gamma-oryzanol (GO) at 0 (C) (control group), 20 (GO20), 40 (GO40), 60 (GO60), 80 (GO80), and 100 (GO100) µM (GON) (CN), (GON20), (GON40), (GON80), (GON100) on post-thawed quality fertility rooster sperm. Sperm motility, plasma membrane integrity, total abnormality, mitochondrial activity (Rhodamine 123), apoptotic features (Annexin V/Propidium...

This experiment was conducted to evaluate the effects of different levels Hatchery Wastes (HW) on performance, tibia ash, blood calcium and phosphorus concentrations in broiler chickens. Birds were fed a corn-soybean meal diet for 7 days. The experimental treatments included corn soybean 3 other containing 1.5, 3.0 4.5% HW. five replicate groups 15 Ross 308 chicks allocated each dietary treatment. Data analyzed completely randomized design. Results indicated that there no significant...

This experiment was conducted to determinate digestible energy and digestibility coefficients of protein, calcium phosphorus malt (germinated barley) barley in broilers nutrition. Four dietary treatments included diet (96.24% diet) three other diets which replacing with as 32.08, 64.16 96.24% (malt were fed from 18-25 days age. Experimental data analyzed a completely randomized design. Results indicated that value nutrient significantly increased experimental (p<0.01). Digestibility higher...

Summary The aim of this study was to evaluate the effects processing lemon pulp with S accharomyces cerevisiae on physical properties, chemical composition, digestion coefficients and blood parameters. Eight adult male R aeini goats were used in a 28‐day period. experimental design completely randomised two treatments four replicates. first 21 days for adaptation, last 7 collecting samples. animals housed individual metabolic cages equipped urine–faeces separator fed diet containing alfalfa...

Abstract The aim of our research was to examine the effects crocin (0.5 (C0.5), 1 (C1) and 1.5 (C1.5) mM) naringenin (50 (N50), 100 (N100) 150 (N150) µM) in cryopreservation extender for freezing rooster semen. Sperm motility, viability, abnormalities, membrane integrity, mitochondrial activity, apoptosis status, lipid peroxidation (LP), GPX, SOD, TAC, mRNA expression pro-apoptotic (CASPASE 3) anti-apoptotic (Bcl-2) genes, fertility hatchability rate were investigated following...