Abstract This pioneering research investigated apigenin potential to augment rooster sperm cryosurvival in an extender model. Apigenin is a natural antioxidant flavonoid showing promise for improved post-thaw function. However, its effects on avian semen cryopreservation remain unexplored. first study supplemented Lake with 0, 50, 100, 200, 400 μmol/L determine the optimal concentrations quality. Supplementation 100 resulted significant enhancements total motility (from 41.5% up 71.5%),...

The aim of our study was to examine the effects crocin (0.5 (C0.5), 1 (C1) and 1.5 (C1.5) mM) naringenin (50 (N50), 100 (N100) 150 (N150) μM) in cryopreservation extender for freezing rooster semen. Sperm motility, viability, abnormalities, membrane functionality, active mitochondria, apoptosis status, lipid peroxidation (LP), GPX, SOD, TAC, mRNA expression pro-apoptotic (CASPASE 3) anti-apoptotic (Bcl-2) genes, fertile eggs, hatched eggs hatching rate were investigated following...

The objective of this study was to uncover genomic regions explaining a substantial proportion the genetic variance in milk production traits and somatic cell score Valle del Belice dairy sheep. Weighted single-step genome-wide association studies (WssGWAS) were conducted for yield (MY), fat (FY), percentage (FAT%), protein (PY), (PROT%), (SCS). In addition, our aim also identify candidate genes within that explained highest proportions variance. Overall, full pedigree consists 5534 animals,...

Colorectal cancer (CRC), which is among the most prevalent cancers worldwide, caused by environmental and genetic factors. It has been shown that p53 gene associated with CRC pathogenesis; moreover, Zyxin (ZYX) may play a role in level activity. Therefore, present research aimed to investigate levels of P53 ZYX genes proteins tumor samples. Cancerous tissues (n=31) matched non-cancerous were randomly obtained from 31 patients CRC. Total RNA was extracted using RNXplus, expressions assessed...

Oxidative stress impairs sperm function and fertility in rams, most notably during cryopreservation, is characterized by compromised motility. Mitochondria contribute immensely towards function, such as mitochondrial membrane potential (MMP) which crucial for ATP production. The purpose of this study was to assess the role MitoPBN supplementation on cryopreserved quality mature Ghezel ram sperm. Semen samples were treated with 0 (control), 100, 150, 200, 250 µmol/L then analyzed key factors....

Abstract. Genetic and phenotypic parameters were estimated for lamb growth traits the Shal sheep using an animal model. Data on performance extracted from available records at Station in Qazvin, Iran. Studied body weights of lambs birth (BW), 3 months age as weaning weight (WW), 6 (6MW), 9 (9MW), yearling (YW), average daily gain to (ADG) Kleiber ratio (KR). Significant random effects each trait determined by fitting additive direct genetic effects, maternal covariance between permanent...

Summary Genetic parameters for birth weight (BW), weaning (WW) and pre‐weaning daily gain (PWDG) in Iranian Mehraban sheep were estimated using restricted maximum likelihood (REML) procedure. Six different animal models fitted, differentiated by including or excluding maternal effects, with without covariance between direct genetic effects. The estimates heritability ranged from 0.26 to 0.53, 0.18 0.32 0.15 0.33 BW, WW PWDG respectively. substantially higher when either environmental,...

The aim of the present study is to investigate effects caloric restriction on liver lead-administered rat. Male Sprague-Dawley rats were randomly divided into two groups: Ad libitum fed group (AL, free access normal rat chow) and (CR, 65% AL animals’ food intake). After 6 weeks, half animals each injected lead acetate other saline. Liver tissue samples collected at end experiments. Glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA), tumor necrosis factor (TNF- α...

The purpose of this study was to examine the effect different rutin concentrations on rams epididymal sperm. A local slaughterhouse provided 50 pair testes from 25 rams. were sent lab at room temperature. Spermatozoa extracted by suspending portions cauda epididymis in tris solution. Ram sperm cryopreserved (in liquid nitrogen) a extender containing 0, 0.5, 0.75, 1, and 1.25 mM. Rutin showed superior total progressive motility, beat cross frequency, straight line velocity, velocity average...

Aging leads to decreased fertility in roosters, which is likely due increased oxidative stress. This study evaluated the antioxidant effects of gallic acid (GA) supplementation on sperm quality and aged roosters. whether GA can mitigate age-related decline. Roosters were randomly assigned to: control, 100 mg/kg GA, or 200 GA. Semen parameters, kinetics, hormone levels, rate, hatchability assessed. semen concentration, membrane integrity viability while decreasing defects versus control (P <...

Genetic parameters for reproductive traits in Zandi sheep were estimated from data records of 5025 lambs 178 sires and 1967 dams collected between the years 1993 2010 using ASReml statistical package. four basic two composite traits. Year lambing age ewe used as fixed effects. Due to repeated record animal direct genetic effects permanent environmental considered random. The heritability estimates conception rate, number born, born alive, alive at weaning, per exposed, weaned exposed low...

The aim of the study was to investigate single nucleotide polymorphisms (SNPs) at 16–17 exon DGAT1 gene in Lori-Bakhtiari sheep (LB) and Zel (Z) breeds provide a foundation for studying relationship with some carcass traits genetic between LB Z breeds. A total 309 were slaughtered weight, backfat thickness, fat-tail percentage, dressing percentage adjusted weight measured. Single polymorphism detected by comparing sequences PCR products, restriction fragment length (RFLP) technique adopted...

The purpose of this study was to investigate the effects rosiglitazone on ram semen after cryopreservation quality thawed sperm. Sperm motility, membrane functionality, viability, total abnormality, acrosome integrity, mitochondrial activity, reactive oxygen species production, ATP content and apoptotic features were assessed thawing. Rosiglitazone at concentration 60 µM resulted in highest (P < 0.05) progressive motility straight-line velocity. percentages average path velocity curvilinear...

The aim of the present study was to investigate effects tempol and straw size on rooster sperm post-thaw quality fertility. Rooster semen cryopreserved in Lake extender containing 0 (control), 5, 10, 15 20 μM (in two different size, 0.25 0.5). percentage total progressive motility, VAP VSL increased 10 µM group. Moreover, led lower ROS compared other groups. lowest apoptotic-like changes detected when treated with tempol. minimum observed group 0.5 size. Straw did not have any significantly...

Ifosfamide (IFO) is a widely used antineoplastic drug with broad-spectrum efficacy against various types of cancer. However, different toxicities associated IFO has limited its use. This study was to establish the prophylactic effects betanin, chrysin and ellagic acid IFO-induced neurotoxicity in rats.Animals were randomly divided into eight groups, control, IFO, + chrysin, acid, groups. Betanin (50 mg/kg, i.p.), (25 i.p.) administered rats once daily for two consecutive days. (500 on third...

Regular mild exercise enhances antioxidant and anti-inflammatory systems of the body. The present study investigates voluntary effects on lead toxicity as a known oxidative stressor. Male Sprague-Dawley rats were randomly divided into 2 groups. Sedentary control: animals housed 7 weeks in regular cages. Exercise group: running wheel equipped cages, that is, animal model exercise. During 7th week, all administered acetate. Blood samples collected at end 6th week (before after...