A5052159065- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Nematode management and characterization studies
- Agronomic Practices and Intercropping Systems
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- Coastal wetland ecosystem dynamics
Huazhong Agricultural University
2015-2024
Sanya University
2024
Shandong Agricultural University
2023
University of Idaho
2015
The symbiotic interaction between legume plants and rhizobia results in the formation of root nodules, which plant cells host harbor thousands nitrogen-fixing rhizobia. Here, a Medicago truncatula nodules with activated defense 1 (nad1) mutant was identified using reverse genetics methods. phenotype characterized cell molecular biology approaches. An RNA-sequencing technique used to analyze transcriptomic reprogramming nad1 nodules. In plants, rhizobial infection propagation threads are...
Abstract Root nodules are major sources of nitrogen for soybean (Glycine max (L.) Merr.) growth, development, production, and seed quality. Symbiotic fixation is time-limited, as the root nodule senesces during reproductive stage plant specifically development. Nodule senescence characterized by induction senescence-related genes, such papain-like cysteine proteases (CYPs), which ultimately leads to degradation both bacteroids cells. However, how senescence–related genes activated in...
Summary Suppression of innate immunity is essential for rhizobial infection and colonization in compatible interactions with leguminous plants. In Medicago nad1 mutant plants, excessively activated, resulting necrotic cell death after rhizobia are released from threads into symbiotic cells, suggesting that plays a critical role regulating bacteroid persistence. this study, we identified three respiratory burst oxidase homologs (Rboh) one calcium‐dependent protein kinase ( CDPK ) as key...
Mechanisms underlying nodulation factor signaling downstream of the receptors (NFRs) have not been fully characterized. In this study, clathrin heavy chain1 (CHC1) was shown to interact with Rho-Like GTPase ROP6, an interaction partner NFR5 in Lotus japonicus. The CHC1 gene found be expressed constitutively all plant tissues and induced Mesorhizobium loti-infected root hairs nodule primordia. When leaves Nicotiana benthamiana, ROP6 were colocalized at cell circumference within cytoplasmic...
Suppression of host innate immunity appears to be required for the establishment symbiosis between rhizobia and plants. In this study, we established a system that included plant, bacterial pathogen symbiotic rhizobium study role during interactions. A pathogenic bacterium, Pseudomonas syringae pv. tomato strain DC3000 (Pst DC3000), was shown cause chlorosis in Medicago truncatula A17. Sinorhizobium meliloti Sm2011 (Sm2011) Pst alone induced similar defense responses M. truncatula. However,...
Phytosulfokine (PSK) is a tyrosine-sulfated peptide that widely distributed in plants, participating cell proliferation, differentiation, and innate immunity. The potential role of PSK nodulation legumes has not been reported. In this work, five precursor genes were identified Lotus japonicas, designated as LjPSK1 to LjPSK5. Three them (LjPSK1, LjPSK4, LjPSK5) found be expressed nitrogen-fixing root nodules. LjPSK4 induced at the early stage nodulation. Interestingly, while expression was...
Expression of Nodule Inception (NIN) is essential for initiation legume-rhizobial symbiosis. An existing model regarding the regulation NIN expression involves two GRAS transcription factors - NSP1 (Nodulation Signaling Pathway 1) and NSP2. NSP2 forms a complex with to directly bind promoter. However, rhizobial treatment-induced could still be detected in nsp1 mutant plants, suggesting that other proteins must involved expression. A combination molecular, biochemical genetic analyses was...
The final phase in root nodule development is senescence. mechanism underlying the initiation of senescence requires further elucidation. In this study, we investigate intrinsic signals governing soybean (Glycine max L. Merr.) senescence, uncovering ethylene as a key signal intricate mechanism. Two AP2/ethylene response factor (ERF) transcription (TF) genes, GmENS1 and GmENS2 (Ethylene-responsive factors required for Nodule Senescence), exhibit heightened expression levels both aged nodules...
Symbiotic nitrogen fixation within nitrogen-fixing clade (NFC) plants is thought to have arisen from a single gain followed by massive losses in the genomes of ancestral non-nodulating plants. However, molecular evidence supporting this model limited. Here, we confirm through bioinformatic analysis that NODULES WITH ACTIVATED DEFENSE1 (NAD1) present only NFC and thus an NFC-specific gene. Moreover, NAD1 was specifically expressed nodules. We identified three conserved nodulation-associated...
Legume-rhizobial symbiosis initiates the formation of root nodules, within which rhizobia reside and differentiate into bacteroids to convert nitrogen ammonium, facilitating plant growth. This process raises a fundamental question: how is immunity modulated nodules when exposed substantial number foreign bacteria? In Medicago truncatula, mutation in NAD1 (Nodules with Activated Defense 1) gene exclusively results necrotic combined activated immunity, underscoring critical role suppressing...
The legume–rhizobial symbiosis is a distinctive model in plant–microbe interactions. Despite being foreign invaders, rhizobia are acknowledged as "friends" by compatible host plants, leading to mutualistic endosymbiosis (Cao et al., 2017). Crucial the establishment of this recognition rhizobial nodulation factor (NF) hetero-receptor complex involving two Lysin Motif receptor kinases (LYKs) namely NF 1 (LjNFR1), member LYK-I clade with kinase activity, and LjNFR5, LYR-I without activity...
Resistance genes (R genes) are a class of that immune to wide range diseases and pests. In planta, NLR essential components the innate system. Currently, belonging family have been found in number plant species, but little is known peach. Here, 286 were identified on peach genome by using their homologous Arabidopsis thaliana as queries. These contained at least one NBS domain LRR domain. Phylogenetic N-terminal analysis showed these NLRs could be separated into four subfamilies (I-IV)...
The calcium/calmodulin-dependent protein kinase CCaMK forms a complex with its phosphorylation target CIP73 (CCaMK-interacting of 73 kDa). In this work, homolog the animal HSC/HSP70 interacting (HIP) was identified as an partner in Lotus japonicus. L. japonicus HIP contains all functional domains characteristic proteins. C-terminal STI1-like domain found to be necessary and sufficient for interaction CIP73. between occurred both nuclei cytoplasm Nicotiana benthamiana leaf cells. interactions...
Abstract The perception of rhizobial nodulation factors (NF) by plant NF receptors is crucial for mediating legume nodulation. In Medicago truncatula nodulation, a proposed two-receptor model designates MtLYK3 as an entry receptor, while other LYK proteins, with unidentified functions, may serve signaling receptors. Here, we generated the single, double, and triple mutants M. MtLYK2, MtLYK3, MtLYK2bis using CRISPR/Cas technology examined their roles in Our findings suggest that all three...
Summary Plant immunity is suppressed in the symbiotic nodule cells, thereby facilitating rhizobial infection. Medicago truncatula NODULES WITH ACTIVATED DEFENSE1 (MtNAD1) crucial for suppressing nodules; however, its molecular function unclear. We explored basis of role MtNAD1 innate M. nodules. mutants lacking MtATG7 produced defective nodules, sharing some similarities with Mtnad1 mutant Furthermore, interacted several immunity‐related proteins, including BAX‐inhibitor1a (MtBI‐1a), two...
Legume-rhizobial symbiosis leads to the formation of root nodules, where rhizobia reside and develop into bacteroids reduce nitrogen ammonium for plant growth, which leaves an opening question as how immunity is attenuated in nodules presence large number foreign bacteria. In Medicago truncatula, mutation NAD1 (Nodules with Activated Defense 1) only produces necrotic overactivated immunity, indicating indispensable component required suppressing nodules. this study, a dual RNA-seq...