Abstract Background Drought is one of the main concerns worldwide and restricts development agriculture. Silicon improves drought resistance plants, but underlying mechanism remains unclear. Results We sequenced transcriptomes both control silicon-treated peach seedlings under stress to identify genes or gene networks that could be managed increase tolerance seedlings. Peach ( Prunus persica ) were used analyse effects silicon on plant growth physiological indexes related stress. The results...

Abstract LATERAL ORGAN BOUNDARIES DOMAIN/ASYMMETRIC LEAVES2-LIKEs (LBDs/ASLs) are plant-specific transcription factors that function downstream of auxin-regulated lateral root (LR) formation. Our previous research found PpLBD16 positively regulates peach (Prunus persica) LR However, the regulatory network and target genes still largely unknown. Here, we constructed a homologous overexpression line silenced line. We overexpressing promoted initiation, while silencing inhibited Through RNA...

A growth trial was conducted to estimate the optimum requirement of dietary zinc (Zn) for grass carp (Ctenopharyngodon idella). Triplicate groups (3.97 ± 0.05 g) were fed diets containing graded levels (13, 25, 34, 53, 89 and 135 mg kg−1) Zn 8 weeks. Grass with higher than 34 kg−1 significantly increased final body weight, weight gain specific rate (P < 0.05). For composition, fish 53 decreased moisture contents but lipid whole liver. Whole body, scales, vertebrae liver mineralization all...

Tandem 3′ untranslated regions (UTRs), produced by alternative polyadenylation (APA) in the terminal exon of a gene, could have critical roles regulating gene networks. Here we profiled tandem poly(A) events on genome-wide scale during embryonic development zebrafish ( Danio rerio ) using recently developed SAPAS method. We showed that 43% expressed protein-coding genes UTRs. The average UTR length follows V-shaped dynamic pattern early embryogenesis, which UTRs are first shortened at...

SNF-related Kinase 1 (SnRK1) is a key component of the cell signaling network. SnRK1 known to respond wide variety stresses, but its exact role in salt stress response and tolerance still largely unknown.In this study, we reported that overexpression gene encoding α subunit Prunus persica (PpSnRK1α) tomato could improve tolerance. The increase PpSnRK1α-overexpressing plants was found correlate with increased PpSnRK1α expression level kinase activity. And lines exhibited lower leaf damage as...

Abstract Fruit quality depends largely on the type and amount of sugar accumulated in fruit. In peach [Prunus persica (L.) Batsch], sorbitol is main photosynthetic product plays a crucial role metabolism. As conserved energy sensor, SNF1-related kinase 1 (SnRK1) involved regulation carbon this study, SnRK1 was able to respond induction by treatment with exogenous trehalose ‘Ruipan 17’ After 100-mM for 3 h, activity decreased 18% activities dehydrogenase (SDH) sucrose synthase (SS) also...

The residues of pharmaceuticals in surface waters megacities and ecotoxicological implications are particular concern. In this study, we combined field investigations model simulations to explore the contamination cardiovascular lipid-lowering drugs, one group most prescribed medications globally, a typical megacity, Shanghai, with high wastewater treatment ratio (≈96%). Among 26 target substances, 19 drugs were detected aqueous concentrations ranging from 0.2 (ketanserin) 715 ng/L...

ABSTRACT Micropterus salmoides rhabdovirus (MSRV) is an important pathogen of largemouth bass. Despite extensive research, the functional receptors MSRV remained unknown. This study identified host protein, laminin receptor (LamR), as a cellular facilitating entry into cells. Our results demonstrated that LamR directly interacts with G playing pivotal role in attachment and internalization processes MSRV. Knockdown siRNA, blocking cells antibody, or incubating virions soluble protein...

In recent years, mobile laser measurement systems have markedly enhanced the capabilities of deformation detection and defect identification within metro tunnels, attributed to their superior efficiency, precision, versatility. Nevertheless, challenges persist, including substantial equipment costs, inadequate after-sales support, technological barriers, limitations in customization. This paper develops a system that has been specifically developed for purpose detecting tunnels. The...

To achieve efficient and sustainable NH3 production by electrochemical nitrogen reduction at room temperature, the catalyst must have high activity superior stability. Here, we prepared a series of two-dimensional BixSby alloy nanosheet materials with different atomic ratios improved coreduction method. Experiments density functional theory (DFT) calculations reveal following. (i) The uniformly distributed catalysts not only larger specific surface area, thereby promoting full exposure Bi...

Microbial fertilizers have a significant role in promoting plant growth, resistance to environmental stresses, and soil remediation. are mainly composed of beneficial microorganisms that contain specific functions. Focusing on the peach tree rhizosphere region, this study aimed isolate screen bacteria with efficient phosphate-solubilizing capacity for application microbial fertilizers, as well dig deeper into other properties strains further explore roles these (PSBs) terms growth order...

A growth trial was conducted to estimate the optimum concentration of dietary available phosphorus (P) for grass carp (Ctenopharyngodon idella). Triplicate groups (5.59 ± 0.02 g) were fed diets containing graded levels (2.36, 4.27, 6.31, 8.36, 10.4 and 14.8 g kg−1) P 8 weeks. Grass with P-supplemented had significantly higher specific rate, weight gain, protein efficiency ratio feed than fish basal diet. In whole-body composition, content increased, while lipid decreased increase in level...

This study was conducted to investigate the effect of dietary manganese (Mn) on growth, vertebrae and whole-body Mn content juvenile grouper, examine copper (Cu), iron (Fe), zinc (Zn), calcium (Ca), phosphorus (P) magnesium (Mg) whole body. Seven casein-gelatin-based diets were supplemented with 0, 5, 10, 15, 20, 50 1000 mg kg−1 from MnSO4·H2O. Grouper an initial weight 12.9 ± 0.4 g fed satiation one seven for 8 weeks. Growth not significantly affected by supplements. Vertebrae increased...

Hydrogen sulfide (H2S) has been shown to regulate many physiological processes of plants. In this study, we observed that 0.2 mM sodium hydrosulfide (NaHS), a donor H2S, can the root architecture peach seedlings, increasing number lateral roots by 40.63%. To investigate specific mechanisms which H2S regulates growth in peach, used RNA sequencing and heterologous expression technology. Our results showed exogenous led 44.50% increase concentration endogenous auxin. Analyses differentially...

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Psychotropic pharmaceuticals account for a large proportion of the most prescribed drugs globally. They cannot be completely removed in wastewater treatment plants (WWTPs), which raise concern about their occurrence and ecotoxicology aquatic system. In present study, we developed sensitive reliable method analyzed 34 psychotropic river water megacity Shanghai. The impacts WWTPs on contamination psychoactive were also evaluated by comparing samples collected from upstream downstream WWTPs....

A 63-day growth trial was undertaken to estimate the effects of supplemented lysine and methionine with different dietary protein levels on performance feed utilization in Grass Carp (Ctenopharyngodon idella). Six plant-based practical diets were prepared, 32CP, 30CP 28CP formulated contain 320 g kg−1, 300 kg−1 280 crude without supplementation. In supplementary group, added formulate 32AA, 30AA 28AA protein, respectively, according whole body amino acid composition Carp. groups...

Gene transcribing with alternative polyadenylation (APA) sites leads to mRNA isoforms, which may encode different proteins or harbor 3'UTRs. APA plays an important role in regulating gene expression network among various physiological processes, such as development, immune responses and cancer. Several methods of library construction for study have been developed apply high-throughput sequencing. However, the requirement high-input RNA time-consuming nature current limited studies samples...

A growth trial was conducted to estimate the optimum concentration of dietary magnesium (Mg) for grass carp (Ctenopharyngodon idella). Triplicate groups (5.56 ± 0.02 g) were fed diets containing graded levels (187, 331, 473, 637, 779 and 937 mg kg−1) Mg 8 weeks. Weight gain, specific rate feed efficiency linearly increased up 637 kg−1 then levelled off beyond this level. For body composition, higher than 473 significantly decreased moisture content but lipid whole body, muscle liver. Dietary...

A growth trial was conducted to estimate the optimum concentration of dietary magnesium (Mg) for grass carp (Ctenopharyngodon idella). Triplicate groups (5.56 ± 0.02 g) were fed diets containing graded levels (187, 331, 473, 637, 779 and 937 mg kg−1) Mg 8 weeks. Weight gain, specific rate feed efficiency linearly increased up 637 mg kg−1 then levelled off beyond this level. For body composition, higher than 473 mg kg−1 significantly decreased moisture content but lipid whole body, muscle...

Abstract Sucrose nonfermentation 1 (SNF1) related kinase (SnRK1) is a central energy sensor in plants and key switch regulating carbon nitrogen metabolism. Fruit quality depends on leaf photosynthetic efficiency carbohydrate accumulation, but the role of peach ( Prunus persica ) SnRK1 α subunit PpSnRK1α metabolism light signal response remains unclear. We studied tomato leaves overexpressing responses ‐overexpressing to signals. Transcriptome, metabolome, real‐time quantitative polymerase...

Resistance genes (R genes) are a class of that immune to wide range diseases and pests. In planta, NLR essential components the innate system. Currently, belonging family have been found in number plant species, but little is known peach. Here, 286 were identified on peach genome by using their homologous Arabidopsis thaliana as queries. These contained at least one NBS domain LRR domain. Phylogenetic N-terminal analysis showed these NLRs could be separated into four subfamilies (I-IV)...