A5002142232- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Cells and Metastasis
- Microfluidic and Bio-sensing Technologies
- Cellular Mechanics and Interactions
- Microfluidic and Capillary Electrophoresis Applications
- Nanoparticle-Based Drug Delivery
- Cancer, Hypoxia, and Metabolism
- Chemical Reactions and Isotopes
- Cell Image Analysis Techniques
- Force Microscopy Techniques and Applications
- Single-cell and spatial transcriptomics
- Gynecological conditions and treatments
- Uterine Myomas and Treatments
- Endometrial and Cervical Cancer Treatments
- Advanced Biosensing Techniques and Applications
- Gene expression and cancer classification
- Tissue Engineering and Regenerative Medicine
- thermodynamics and calorimetric analyses
- Pluripotent Stem Cells Research
- Machine Learning in Materials Science
- Electrowetting and Microfluidic Technologies
Tsinghua–Berkeley Shenzhen Institute
2020-2024
Tsinghua University
2020-2024
Prolyl hydroxylases (PHD) inhibitors have been observed to improve drug distribution in mice tumors via blood vessel normalization, increasing the effectiveness of chemotherapy. These effects are yet be demonstrated human cell models. Tumor spheroids three-dimensional clusters that great potential evaluation for personalized medicine. Here, we used a perfusable vascularized tumor spheroid-on-a-chip simulate microenvironment vivo and PHD inhibitor dimethylallyl glycine prevents degradation...
In vitro organoids, including cerebral are usually developed without mechanical compression, which may contribute to a delay in maturation. Here, we present protocol for encapsulating organoids with thin shell of low-concentration alginate hydrogel. We describe steps organoid generation, microfluidic chip culture, Matrigel coating, expansion and encapsulation. then detail procedures maturation culture characterization. The moderate compressive stimulation that the provides promotes cell...
Abstract Endometrial injury and intrauterine adhesions are increasingly reported in recent years; however, treatment options remain limited. Intravenous injection of mesenchymal stem cells (MSCs) for endometrium regeneration has limited effectiveness as the retention rate transplanted is low. Hydrogel‐based tissue‐engineered solutions, such MSC‐seeded bioscaffolds, to increase rates; a less invasive alternative still desirable. 560‐µm homogeneous Matrigel microspheres fabricated, loading...
Current organoid technology faces challenges such as low throughput and limited automation, which leads to inconsistencies in development hinders clinical applications. The ability handle ultra-small samples greatly restricts the use of organoids settings. Our research introduces OrgFab, an integrated bioprinter that is able manipulate down 5 μL accurately print onto 384-well plates. A 10-μL bioink produces around 100 precursors, enough for one batch drug testing. instrument operates a fully...
Abstract Tumor spread is responsible for most deaths related to cancer. Increasing the accuracy of cancer prognosis critical reducing high mortality rates in patients. Here, we report that electrostatic potential difference (EPD) between tumor and its paratumor tissue a prognostic marker spread. This finding concluded from patient‐specific EPD values clinical observation. The were measured on cryosections 51 patients using Kelvin probe force microscopy (KPFM). A total ~44% (15/34) V...
Abstract Organoid technology has developed at an impressive speed during the past decade. Still, organoids are not widely used in practical applications as expected. It is believed that this translation can be greatly accelerated with integration of engineering and artificial intelligence into current research practices. proposed approach missing link to realize key milestones organoid technology, namely, high‐throughput, homogeneous, standardized production, automated manipulation,...
Current administrations for precision drug uses are limited in evaluation speed. Here, we propose the use of multiplex gene-based digital markers extremely rapid personalized prediction individual sensitivity to cancer drugs. We first screen transcriptional profiles by applying two three gene filters and scoring genes their impact on finalize lists K-nearest neighbors cross-validation. The type dependent, composed tens hundreds expressions, rapidly quantified reverse transcription...
Automated segmentation and evaluation algorithms have been demonstrated to enhance the simplicity translational utility of organoid technology. However, there is a pressing need for development complex organoids that possess epithelium environmental elements, dense regional cell aggregation, intraorganoid morphologies. Nevertheless, has limited progress, including both construction data sets algorithms, in use user-friendly microscopy address such organoids. In this study, set bright-field...
Abstract In traditional reaction flasks, the rate of macromolecular compounds is dictated by concentrations and distribution reactants along with their intrinsic kinetics. Controlling kinetics in microfluidic droplets has been proposed through regulation flow dynamics, but yet to be demonstrated experimentally. Here, this hypothesis verified accelerating or suppressing reactant mixing droplets. The control modulation topology microdroplets may enable further developments modeling systems.
Tumor spread is responsible for most deaths related to cancer. Increasing the accuracy of cancer prognosis could be key reduce high mortality rates in patients. Here, we report that electrostatic potential difference between tumor and paratumor tissue cryosections serves as a prognostic marker spread. We demonstrated polarity this correlated with patients abundance stem cells tissues. Then, using spheroids mimic microenvironment, showed selectively migrate toward negatively charged...
In article number 2000309, Shaohua Ma and co-workers illustrate the modulation of macromolecular reaction kinetics via flow topology control, instead chemical approaches. They offer a strategy to design novel systems understand intracellular reactions.
Prolyl hydroxylases (PHD) inhibitors have been observed to normalise blood vessels and increase drug distribution in mice tumour, which increases the effectivity of chemotherapy. However, these effects are yet be demonstrated human models. Herein, we developed a vascularised tumouroid-on-a-chip that closely resembles tumour microenvironment vivo demonstrate addition PHD inhibitor dimethyloxallyl glycine (DMOG) prevents degradation normal enhances efficacy chemotherapy drugs paclitaxel...
Organoids Biomedical Translation In article 2100535, Ma and co-workers describe their perspective on the engineering elements to promote organoid applications in biomedical translation. “Organoid engineering” comprises conceptual modules of high-throughput production with reduced variability, automated culturing a hyper-parallel manner, intelligent evaluation by learning algorithms process multi-modality data, finally, “instrumentation” catalyze translation technologies.