Abstract The complete sequence of the mitochondrial genome screwworm Cochliomyia hominivorax was determined. This is 16 022 bp in size and corresponds to a typical Brachycera mtDNA. A Serine start codon for COI incomplete termination codons COII, NADH 5 4 genes were described. nucleotide composition C. mtDNA 77% AT‐rich, reflected predominance AT‐rich protein‐coding genes. Non‐optimal usage commonly observed Phylogenetic analysis distributed Acalypterate species as monophyletic group...

Summary This study reports the molecular characterization of mtDNA control region (called A + T‐rich in insects) five dipteran species which cause myiasis: Cochliomyia hominivorax Coquerel, macellaria Fabricius, Chrysomya megacephala Lucilia eximia Wiedemann (Diptera: Calliphoridae) and Dermatobia hominis Linnaeus Jr Oestridae). The these varies length from 1000 to 1600 bp. Two structural domains with specific evolutionary patterns were identified. These (1) conserved sequence blocks...

Abstract Summary: The Arthropodan Mitochondrial Genomes Accessible database (AMiGA) is a relational developed to help in managing access the increasing amount of data arising from developments arthropodan mitochondrial genomics (136 genomes as September 2005). strengths AMiGA include (1) more accessible and up-to-date containing comprehensive set for this phylum, (2) provision flexible search options retrieving detailed information such bibliographical data, genomic graphics, FASTA sequences...

Abstract Mitochondrial DNA (mtDNA) sequences from eight species of myiasis‐causing flies, stored for up to 50 years, were amplified successfully. Universal primers used amplify six specific regions total genomic DNA, including five mtDNA genes. The comparison phenol/chloroform, DNAzol ® and Chelex techniques extraction showed that the reagent was most efficient in retrieving museum specimens, although procedure is currently frequently reported method. Comparison universal primer with...

The primary screwworm fly Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) is one of the most important insect pests livestock in neotropical regions, whereas macellaria (Fabricius) Calliphoridae), secondary screwworm, medical and sanitary importance because its role dissemination pathogens. These two species share morphological similarities both may occur same myiasis, but different developmental stages. In this work, usefulness PCR-RFLP (polymerase chain reaction - restriction...

The identification of insect species involved in corpses decomposition is particular importance estimating the post-mortem interval (PMI) forensic science, since PMI based on information about life cycle necrophagous insects. However, some species, especially their immature stages, may be complicated by many factors, even for experienced taxonomists. Species same genus such as Hemilucilia segmentaria (Fabricius) and H. semidiaphana (Rondani) (Diptera: Calliphoridae) are morphologically...

This work describes the molecular characterization of cytochrome c oxidase subunit I (COI) gene mitochondrial DNA from three species great medical and veterinary importance: horn fly, Haematobia irritans, stable Stomoxys calcitrans house Musca domestica (Diptera: Muscidae) (Linnaeus). The nucleotide sequence in all was 1536 bp size coded for a 512 amino acid peptide. bias an A+T-rich is linked to features: high A+T content throughout entire gene, third codon position, predominance codons. An...

Abstract The amplification of complete mitochondrial genomes by long PCR (polymerase chain reaction) has been a major contribution to the large‐scale sequencing arthropodan genomes. In this work, we designed six conserved long‐PCR primers successfully recover entire genome horn fly Haematobia irritans (Diptera: Muscidae) in two overlapping fragments. conservation and versatility these were tested for 17 other species from four insect orders: Diptera (14), Coleoptera (1), Lepidoptera (1)...

Despite the successful use of universal primers for amplifying insect mtDNA, specific regions remain difficult to recover and demand taxon-specific primers. In this work, we describe a new set efficiently sequencing mtDNA control region three tRNA gene clusters dipterans medical veterinary importance, including Muscidae, Calliphoridae, Oestridae species. These were useful investigating nucleotide information structural organization dipteran mtDNA.

Despite the successful use of universal primers for amplifying insect mtDNA, specific regions remain difficult to recover and demand taxon-specific primers. In this work, we describe a new set efficiently sequencing mtDNA control region three tRNA gene clusters dipterans medical veterinary importance, including Muscidae, Calliphoridae, Oestridae species. These were useful investigating nucleotide information structural organization dipteran mtDNA.

The horn fly, Haematobia irritans (L.) (Diptera: Muscidae), is a cosmopolitan livestock pest that has caused great negative impact on the animal production sector throughout world. Here, we describe 10 polymorphic microsatellite loci isolated from H. irritans. number of alleles found ranged two to eight per locus and expected heterozygosity 0.1421 0.7702. These are potentially useful for fine-scale genetic characterization fly populations provide fundamental information management planning...