A5035193301- CRISPR and Genetic Engineering
- Cocoa and Sweet Potato Agronomy
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Phytoplasmas and Hemiptera pathogens
- RNA Interference and Gene Delivery
- Legume Nitrogen Fixing Symbiosis
- Forensic Entomology and Diptera Studies
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Insect and Pesticide Research
- RNA modifications and cancer
- Botanical Research and Applications
- Food Chemistry and Fat Analysis
- Genetics and Neurodevelopmental Disorders
- Protein Degradation and Inhibitors
- Fungal Biology and Applications
- Acute Myeloid Leukemia Research
- Soybean genetics and cultivation
- Insect symbiosis and bacterial influences
- Prenatal Screening and Diagnostics
- Plant Pathogens and Fungal Diseases
- Lignin and Wood Chemistry
- Phytochemical compounds biological activities
- African Botany and Ecology Studies
Institute of Molecular Biology
2020-2024
Institut Curie
2015-2017
Inserm
2015-2016
Centre National de la Recherche Scientifique
2015-2016
Université Paris Sciences et Lettres
2016
Universidade Estadual de Campinas (UNICAMP)
2005-2014
Combating parasitic DNA by methylation plays an important role in repressing the expression of “parasitic” DNAs, such as transposable elements, which have invaded our genomes. Mammals three methyltransferase enzymes. Barau et al. discovered a fourth enzyme mice. The DNMT3C is duplication DNMT3B and found male germ cells. There it targets evolutionarily young transposons, there heavy burden mouse genome. methylates silences preserving fertility. Science , this issue p. 909
Transcription poses a threat to genomic stability through the formation of R-loops that can obstruct progression replication forks. are three-stranded nucleic acid structures formed by an RNA-DNA hybrid with displaced non-template DNA strand. We developed Proximity Proteomics map R-loop proximal proteome human cells using quantitative mass spectrometry. implicate different cellular proteins in regulation and identify role tumor suppressor DDX41 opposing double strand break accumulation...
DNA methylation is essential for protecting the mammalian germline against transposons. When methylation-based transposon control defective, meiotic chromosome pairing consistently impaired during spermatogenesis: How and why meiosis vulnerable to activity unknown. Using two methylation-deficient backgrounds, Dnmt3L Miwi2 mutant mice, we reveal that largely dispensable silencing transposons before onset. After this, it becomes crucial back up a developmentally programmed H3K9me2 loss....
Epigenetic variation, such as heritable changes of DNA methylation, can affect gene expression and thus phenotypes, but examples natural epimutations are few little is known about their stability frequency in nature. Here, we report that the Qua-Quine Starch (QQS) Arabidopsis thaliana, which involved starch metabolism originated de novo recently, subject to frequent epigenetic variation Specifically, show this varies considerably among accessions well within populations directly sampled from...
Moniliophthora perniciosa is a hemibiotrophic fungus that causes witches' broom disease (WBD) in cacao. Marked dimorphism characterizes this fungus, showing monokaryotic or biotrophic phase symptoms and later dikaryotic saprotrophic phase. A combined strategy of DNA microarray, expressed sequence tag, real-time reverse-transcriptase polymerase chain reaction analyses was employed to analyze differences between these two fungal stages vitro. In all, 1,131 putative genes were hybridized with...
The PIWI-interacting RNA (piRNA) pathway guides the DNA methylation of young, active transposons during germline development in male mice
The necrosis- and ethylene-inducing peptide 1 (NEP1)-like proteins (NLPs) are secreted from bacteria, fungi oomycetes, triggering immune responses cell death in dicotyledonous plants. Genomic-scale studies of Moniliophthora perniciosa, the fungus that causes Witches' Broom disease cacao, which is a serious economic concern for South Central American crops, have identified five members this family (termed MpNEP1-5). Here, we show by RNA-seq MpNEP2 virtually only NLP expressed during...
Witches' broom disease (WBD) of cacao differs from other typical hemibiotrophic plant diseases by its unusually long biotrophic phase. Plant carbon sources have been proposed to regulate WBD developmental transitions; however, nothing is known about their availability at the plant–fungus interface, apoplastic fluid cacao. Data are provided supporting a role for dynamics soluble in prompting end phase infection. Carbon depletion and consequent fungal sensing starvation were identified as key...
The hemibiotrophic basidiomycete fungus Moniliophthora perniciosa, the causal agent of Witches' broom disease (WBD) in cacao, is able to grow on methanol as sole carbon source. In plants, one main sources pectin present structure cell walls. Pectin composed highly methylesterified chains galacturonic acid. hydrolysis between methyl radicals and acid esterified pectin, mediated by a methylesterase (PME), releases methanol, which may be decomposed oxidase (MOX). analysis M. pernciosa genome...
This report describes the cloning, sequence and expression analysis of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene Moniliophthora perniciosa, most important pathogen cocoa in Brazil. Southern blot revealed presence a single copy GAPDH M. perniciosa genome (MpGAPDH). The complete MpGAPDH coding contained 1,461 bp with eight introns that were conserved genes other basidiomycete species. cis-elements promoter region similar to those basidiomycetes. Likewise, encoded putative 339 amino...
Abstract The amplification of complete mitochondrial genomes by long PCR (polymerase chain reaction) has been a major contribution to the large‐scale sequencing arthropodan genomes. In this work, we designed six conserved long‐PCR primers successfully recover entire genome horn fly Haematobia irritans (Diptera: Muscidae) in two overlapping fragments. conservation and versatility these were tested for 17 other species from four insect orders: Diptera (14), Coleoptera (1), Lepidoptera (1)...
Abstract DNA methylation plays a critical role in spermatogenesis, as evidenced by the male sterility of methyltransferase (DNMT) mutant mice. Here, we report striking division labor establishment landscape germ cells and its functions spermatogenesis: while DNMT3C is essential for preventing retrotransposons from interfering with meiosis, DNMT3A broadly methylates genome—at exception DNMT3C-dependent retrotransposons—and controls spermatogonial stem cell (SSC) plasticity. By reconstructing...