A5025048968- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Light effects on plants
- RNA Research and Splicing
- Circadian rhythm and melatonin
- DNA Repair Mechanisms
- Chromatin Remodeling and Cancer
- Acute Myeloid Leukemia Research
- Genomics and Chromatin Dynamics
- Photoreceptor and optogenetics research
- Ubiquitin and proteasome pathways
- Genetics and Neurodevelopmental Disorders
- Signaling Pathways in Disease
- Plant Molecular Biology Research
- Protein Degradation and Inhibitors
- Neurobiology and Insect Physiology Research
- Cancer-related gene regulation
Johannes Gutenberg University Mainz
2025
Institute of Molecular Biology
2021-2025
University of Würzburg
2024
University of Milano-Bicocca
2019-2021
Institute of Molecular Biology
2021
University of Virginia
2003-2007
RNA-binding proteins (RBPs) are emerging as important effectors of the cellular DNA damage response (DDR). The RBP FUS is implicated in RNA metabolism and repair, it undergoes reversible liquid-liquid phase separation (LLPS) vitro. Here, we demonstrate that FUS-dependent LLPS necessary for initiation DDR. Using laser microirradiation FUS-knockout cells, show required recruitment to sites DDR factors KU80, NBS1, 53BP1 SFPQ, another relocation SFPQ similarly impaired by inhibitors, or...
Transcription poses a threat to genomic stability through the formation of R-loops that can obstruct progression replication forks. are three-stranded nucleic acid structures formed by an RNA-DNA hybrid with displaced non-template DNA strand. We developed Proximity Proteomics map R-loop proximal proteome human cells using quantitative mass spectrometry. implicate different cellular proteins in regulation and identify role tumor suppressor DDX41 opposing double strand break accumulation...
During early transcription, RNA polymerase II (RNAPII) undergoes a series of structural transitions controlled by cyclin-dependent kinases. How protein ubiquitylation and proteasomal degradation control the function RNAPII is less well understood. Here we show that deubiquitinating enzyme USP11 forms complex with TCEAL1, member TFIIS (TCEA)-like family. TCEAL1 shares sequence homology interaction domain elongation factor (which controls fate backtracked RNAPII) competes for binding to core...
SWItch/sucrose non-fermenting (SWI/SNF) complexes are a family of chromatin remodelers that conserved across eukaryotes. Mutations in subunits SWI/SNF cause multitude different developmental disorders humans, most which have no current treatment options. Here, we identify an alanine-to-valine–causing mutation the subunit snfc-5 ( SMARCB1 humans) prevents embryonic lethality Caenorhabditis elegans nematodes harboring loss-of-function swsn-1 SMARCC1/2 humans). Furthermore, found combination...
Circadian rhythms control the temporal arrangement of molecular, physiological, and behavioral processes within an organism also synchronize these with external environment. A cell autonomous molecular oscillator, consisting interlocking transcriptional/translational feedback loops, drives approximately 24-hour duration rhythms. The cryptochrome protein (CRY) plays a central part in negative loop clock by translocating to nucleus repressing CLOCK BMAL1, two transcription factors that...
Abstract RNA-binding proteins (RBPs) are emerging as important effectors of the cellular DNA damage response (DDR). The RBP FUS is implicated in RNA metabolism and repair, it undergoes reversible liquid-liquid phase separation (LLPS) vitro . Here, we demonstrate that FUS-dependent LLPS necessary for initiation DDR. Using laser microirradiation FUS-knockout cells, show required recruitment to sites DDR factors KU80, NBS1, 53BP1, SFPQ, another relocation SFPQ similarly impaired by inhibitors,...
Abstract During early transcription, RNA polymerase II (RNAPII) undergoes a series of structural transitions controlled by cyclin-dependent kinases. Whether protein ubiquitylation and proteasomal degradation affect the fate RNAPII close to promoters is less well understood. Here we show that deubiquitylating enzyme USP11 its heterodimeric partner USP7 form trimeric complex with TCEAL1, member poorly understood TCEAL (TCEA/TFIIS-like) family. TCEAL1 shares sequence homology interaction domain...
RNA-binding proteins (RBPs) are emerging as important effectors of the cellular DNA damage response (DDR). The RBP FUS is implicated in RNA metabolism and repair, it undergoes reversible liquid-liquid phase separation (LLPS) vitro. Here, we demonstrate that FUS-dependent LLPS necessary for initiation DDR. Using laser microirradiation FUS-knockout cells, show required recruitment to sites DDR factors KU80, NBS1, 53BP1, SFPQ, another relocation SFPQ similarly impaired by inhibitors, or...
Abstract Transcription can pose a threat to genomic stability through the formation of R-loops that obstruct progression replication forks. are three-stranded nucleic acid structures formed by an RNA-DNA hybrid with displaced non-template DNA strand. We developed RDProx identify proteins regulate in human cells. relies on expression hybrid-binding domain (HBD) Ribonuclease H1 (RNaseH1) fused ascorbate peroxidase (APEX2) and permits mapping R-loop proximal proteome using quantitative mass...
Abstract SWItch/Sucrose Non-Fermenting (SWI/SNF) complexes are a family of chromatin remodellers that conserved across eukaryotes. Mutations in subunits SWI/SNF cause multitude different developmental disorders humans, most which have no current treatment options. Here we identify an alanine to valine causing mutation the subunit snfc-5 ( SMARCB1 humans) prevents embryonic lethality C. elegans nematodes harbouring loss-of-function swsn-1 SMARCC1/2 humans). Furthermore, found combination this...