A5061966729- MicroRNA in disease regulation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Connective tissue disorders research
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Hedgehog Signaling Pathway Studies
- Cancer-related molecular mechanisms research
- Wnt/β-catenin signaling in development and cancer
- Chromosomal and Genetic Variations
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Congenital heart defects research
- Craniofacial Disorders and Treatments
- Circular RNAs in diseases
- Fetal and Pediatric Neurological Disorders
University of East Anglia
2021-2025
Norwich Research Park
2021-2025
University of Milano-Bicocca
2019-2021
RNA-binding proteins (RBPs) are emerging as important effectors of the cellular DNA damage response (DDR). The RBP FUS is implicated in RNA metabolism and repair, it undergoes reversible liquid-liquid phase separation (LLPS) vitro. Here, we demonstrate that FUS-dependent LLPS necessary for initiation DDR. Using laser microirradiation FUS-knockout cells, show required recruitment to sites DDR factors KU80, NBS1, 53BP1 SFPQ, another relocation SFPQ similarly impaired by inhibitors, or...
Abstract Low-density lipoprotein receptor-related protein 1 (LRP1) is a multifunctional endocytic receptor whose dysfunction linked to developmental dysplasia of the hip, osteoporosis and osteoarthritis. Our work addresses critical question how these skeletal pathologies emerge. Here, we show abundant expression LRP1 in progenitor cells at mouse embryonic stage E10.5 onwards, especially perichondrium, stem cell layer surrounding developing limbs essential for bone formation. Lrp1 deficiency...
Neural crest (NC) cells form a multipotent stem cell population specified during neurulation, which undergo an epithelial-to-mesenchymal transition (EMT) and migrate extensively in the developing embryo, to generate numerous tissues types including craniofacial skeleton, peripheral nervous system pigment cells. The genetic molecular details of NC specification are governed by complex, yet still partially understood gene regulatory network (NC-GRN). In particular, precise function microRNAs...
Abstract RNA-binding proteins (RBPs) are emerging as important effectors of the cellular DNA damage response (DDR). The RBP FUS is implicated in RNA metabolism and repair, it undergoes reversible liquid-liquid phase separation (LLPS) vitro . Here, we demonstrate that FUS-dependent LLPS necessary for initiation DDR. Using laser microirradiation FUS-knockout cells, show required recruitment to sites DDR factors KU80, NBS1, 53BP1, SFPQ, another relocation SFPQ similarly impaired by inhibitors,...
In recent years CRISPR-Cas9 knockouts (KO) have become increasingly ultilised to study gene function. MicroRNAs (miRNAs) are short non-coding RNAs, 20-22 nucleotides long, which affect expression through post-transcriptional repression. We previously identified miRNAs-196a and -219 as implicated in the development of Xenopus neural crest (NC). The NC is a multipotent stem-cell population, specified during early neurulation. Following EMT, cells migrate various points developing embryo where...
RNA-binding proteins (RBPs) are emerging as important effectors of the cellular DNA damage response (DDR). The RBP FUS is implicated in RNA metabolism and repair, it undergoes reversible liquid-liquid phase separation (LLPS) vitro. Here, we demonstrate that FUS-dependent LLPS necessary for initiation DDR. Using laser microirradiation FUS-knockout cells, show required recruitment to sites DDR factors KU80, NBS1, 53BP1, SFPQ, another relocation SFPQ similarly impaired by inhibitors, or...