A5042711524- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Neurogenetic and Muscular Disorders Research
- Amyotrophic Lateral Sclerosis Research
- RNA regulation and disease
- DNA Repair Mechanisms
- Adipose Tissue and Metabolism
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Viral Infections and Immunology Research
- interferon and immune responses
- Fungal and yeast genetics research
- RNA Interference and Gene Delivery
- Bat Biology and Ecology Studies
- Ubiquitin and proteasome pathways
- Alzheimer's disease research and treatments
- MicroRNA in disease regulation
- TGF-β signaling in diseases
- Kruppel-like factors research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Parkinson's Disease Mechanisms and Treatments
- Developmental Biology and Gene Regulation
- Chronic Myeloid Leukemia Treatments
- Dietary Effects on Health
University of Milano-Bicocca
2013-2023
University of Milan
2013-2018
University of Bern
2010
University of Basel
1996-2005
San Raffaele University of Rome
1997
European Molecular Biology Laboratory
1989-1990
European Bioinformatics Institute
1990
The receptor for advanced glycation endproducts (RAGE) mediates responses to cell danger and stress. When bound by its many ligands (which include endproducts, certain members of the S100/calgranulin family, extracellular high-mobility group box 1, integrin Mac-1, amyloid beta-peptide fibrils), RAGE activates programs responsible acute chronic inflammation. is therefore also involved in cancer progression, diabetes, atherosclerosis, Alzheimer's disease. has several isoforms deriving from...
RNA-binding proteins (RBPs) are emerging as important effectors of the cellular DNA damage response (DDR). The RBP FUS is implicated in RNA metabolism and repair, it undergoes reversible liquid-liquid phase separation (LLPS) vitro. Here, we demonstrate that FUS-dependent LLPS necessary for initiation DDR. Using laser microirradiation FUS-knockout cells, show required recruitment to sites DDR factors KU80, NBS1, 53BP1 SFPQ, another relocation SFPQ similarly impaired by inhibitors, or...
Liquid-liquid phase separation (LLPS) of proteins and RNAs has emerged as the driving force underlying formation membrane-less organelles. Such biomolecular condensates have various biological functions been linked to disease. The protein Fused in Sarcoma (FUS) undergoes LLPS mutations FUS causally motor neuron disease Amyotrophic Lateral Sclerosis (ALS-FUS). followed by aggregation cytoplasmic proposed be a crucial mechanism. However, it is currently unclear how impacts behaviour cells,...
Cleavage and polyadenylation specificity factor (CPSF), a key component of the mammalian RNA 3'-end processing machinery, consists four subunits 160, 100, 73, 30 kD. Here we report isolation characterization cDNA encoding 30-kD polypeptide. Antibodies raised against this protein inhibit cleavage coimmunoprecipitate other CPSF subunits. The sequence contains five C3H-zinc-finger repeats putative RNA-binding zinc knuckle motif at carboxyl terminus. Consistent with observation, in vitro...
Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurological disorder that characterized by progressive degeneration of motor neurons (MNs). The pathomechanism underlying the disease largely unknown, even though increasing evidence suggests RNA metabolism, including microRNAs (miRNAs) may play an important role. In this study, human ALS induced pluripotent stem cells were differentiated into MN progenitors and their miRNA expression profiles compared to those healthy control cells....
Mammalian cleavage factor I (CF m ) is an essential that required for the first step in pre-mRNA 3′ end processing. Here, we characterize CF 68 subnuclear distribution and mobility. Fluorescence microscopy reveals addition to paraspeckles accumulates structures partially overlap with nuclear speckles. Analysis of synchronized cells shows speckles varies during cell cycle. At ultrastructural level, associated perichromatin fibrils, sites active transcription, concentrates interchromatin...
Export of mRNA from the nucleus is linked to proper processing and packaging into ribonucleoprotein complexes. Although several observations indicate a coupling between 3' end formation export, it not known how these two processes are mechanistically connected. Here, we show that subunit mammalian pre-mRNA complex, CF I(m)68, stimulates export. I(m)68 shuttles cytoplasm in transcription-dependent manner interacts with export receptor NXF1/TAP. Consistent idea may act as novel adaptor for...
Mitochondrial dysfunction has been implicated in the pathogenesis of a number neurodegenerative disorders including Parkinson, Alzheimer, and Amyotrophic Lateral Sclerosis (ALS). In addition, aberrant mRNA splicing documented neurodegeneration. To characterize cellular response to mitochondrial perturbations at level gene expression alternative pre-mRNA we used splicing-sensitive microarrays profile human neuroblastoma SH-SY5Y cells treated with paraquat, neurotoxic herbicide that induces...
Alternative splicing of terminal exons increases transcript and protein diversity. How physiological pathological stimuli regulate the choice between alternative is, however, largely unknown. Here, we show that Brahma (BRM), ATPase subunit hSWI/SNF chromatin-remodeling complex interacts with BRCA1/BARD1, which ubiquitinates 50 kDa 3΄ end processing factor CstF. This results in inhibition cleavage at proximal poly(A) site a shift towards inclusion distal exon. Upon oxidative stress, BRM is...