A5008706806- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- Prenatal Screening and Diagnostics
- Renal and related cancers
Inserm
2022-2024
Institut Curie
2022-2024
Centre National de la Recherche Scientifique
2022-2024
Université Paris Sciences et Lettres
2022-2024
Université de Montréal
2023-2024
ABSTRACT Somatic DNA methylation is established early during mammalian development, as embryonic cells transition from naive to primed pluripotency. This precedes the emergence of three somatic germ layers, but also segregation germline that undergoes genome-wide demethylation after specification. While essential for embryogenesis, point at which it becomes critical differentiation and whether all lineages equally depend on unclear. Using culture modeling cellular transitions, we found...
Abstract In mammals, 5 methyl-cytosine (5mC) and Polycomb Repressive Complex 2 (PRC2)-deposited histone 3 lysine 27 trimethylation (H3K27me3) are generally mutually exclusive at CpG-rich regions. As mouse embryonic stem cells exit the naïve pluripotent state, there is a massive gain of 5mC coincident with restriction broad H3K27me3 to 5mC-free, To formally assess how shapes landscape, we profiled epigenome differentiated in presence absence DNA methylation machinery. Surprisingly, found that...