A5076218863- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Aluminum toxicity and tolerance in plants and animals
- Plant Micronutrient Interactions and Effects
- Legume Nitrogen Fixing Symbiosis
- Heavy metals in environment
- Plant nutrient uptake and metabolism
- Glycosylation and Glycoproteins Research
- Mechanisms of cancer metastasis
- Plant tissue culture and regeneration
- Plant responses to water stress
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Plant Growth Enhancement Techniques
- Crop Yield and Soil Fertility
- Heavy Metals in Plants
Chinese Academy of Sciences
2012-2023
State Key Laboratory of Plant Genomics
2016-2023
University of Chinese Academy of Sciences
2017-2023
Center for Agricultural Resources Research
2011-2023
Institute of Genetics and Developmental Biology
2011-2023
• Under cadmium (Cd) stress, Solanum nigrum accumulated threefold more Cd in its leaves and was tolerant to Cd, whereas low Cd-accumulating relative, torvum, suffered reduced growth marked oxidative damage. However, the physiological mechanisms that are responsible for differential accumulation tolerance between two species largely unknown. Here, involvement of antioxidative capacity organic amino acids response stress were assessed. contains higher than does S. torvum under toxicity....
• Solanum nigrum is a cadmium (Cd) accumulator, whereas torvum low Cd-accumulating plant. The molecular mechanisms that are responsible for differential accumulation in the two species poorly understood. Here, grafting experiments confirmed increased Cd loading into root xylem was species. An iron (Fe) supply assay indicated Fe S. leaves related to its sensitivity. Transcriptome analyses revealed higher expression of genes encode several metal transporters as well antioxidant-related genes,...
Ubiquitination modulates nearly all aspects of plant life. Here, we reconstituted the Arabidopsis thaliana ubiquitination cascade in Escherichia coli using a synthetic biology approach. In this system, proteins are expressed and then immediately participate reactions within E. cells. Additionally, purification individual components prior to setting up is omitted. To establish co-expressed ubiquitin (Ub) substrates with E1, E2 E3 enzymes Duet expression vectors. The functionality system was...
Receptor-like kinases (RLKs) play important roles in plant immunity signaling; thus, many are hijacked by pathogen effectors to promote successful pathogenesis. Xanthomonas oryzae pv. (Xoo) is the causal agent of rice leaf blight disease. The strain PXO99A has 18 non-TAL (transcription activation-like) effectors; however, their mechanisms action and host target proteins remain largely unknown. Although effector XopR from Xoo MAFF311018 was shown suppress PAMP-triggered immune responses...
Abstract Pathogen-associated molecular patterns (PAMPs) trigger plant innate immunity that acts as the first line of inducible defense against pathogen infection. A receptor-like cytoplasmic kinase BOTRYTIS-INDUCED KINASE 1 (BIK1) functions a signaling hub immediately downstream multiple pattern recognition receptors (PRRs). It is known PLANT U-BOX PROTEIN 25 (PUB25) and PUB26 ubiquitinate BIK1 mediate degradation. However, how homeostasis maintained not fully understood. Here, we show two...
Arabidopsis AVRPPHB SUSCEPTIBLE1 (PBS1) serves as a "decoy" in activating RESISTANCE TO PSEUDOMONAS SYRINGAE5 (RPS5) upon cleavage by Pseudomonas phaseolicola B (AvrPphB), syringae effector. The SEMPH motif PBS1 was thought to allow it be distinguished RPS5 from the closely related kinases. However, underlying mechanism is not fully understood. Here, we isolated and characterized wheat homolog, TaPBS1. Although this plasma membrane-localized kinase could cleaved AvrPphB associate with RPS5,...
Summary The plant hormones brassinosteroids ( BR s) modulate growth and development. Cysteine (Cys) residues located in the extracellular domain of a protein are importance for structure by forming disulfide bonds. To date, systematic study functional significance Cys ‐insensitive 1 BRI 1) is still lacking. We used brassinolide‐induced exogenous bri1 ‐ EMS ‐Suppressor BES dephosphorylation Arabidopsis thaliana protoplasts as readout, took advantage dramatic decrease levels during protoplast...
本研究选取小麦品种“沧6001”(抗盐性品种)和“石4185”(盐敏感品种)为试验材料, 采用水培方法, 对加入蔗糖后两个小麦品种的耐盐性进行比较。结果表明, 盐胁迫下, “沧6001”和“石4185”小麦根系生长均受到抑制, “石4185”根系受到抑制的程度大于“沧6001”; 蔗糖预处理可以显著缓解盐胁迫对根系生长的抑制作用。蔗糖预处理12 h后, “沧6001”和“石4185”的可溶性糖含量上升, 与“石4185”相比, “沧6001”具有更高的可溶性糖水平, 但是蔗糖预处理后“石4185”可溶性糖含量的增加量高于“沧6001”。H2DCFDA荧光探针检测和PI荧光染色检测结果表明, 蔗糖预处理可有效降低盐胁迫下小麦幼苗体内活性氧累积水平, 保护细胞膜的完整性并减小根尖细胞死亡率。蔗糖预处理可以提高小麦幼苗SOD和APX(<i>P<</i>0.05)、CAT活性(<i>P</i>>0.05)。本研究对于理解外源物质对小麦耐盐性影响的生理机制及筛选耐盐性的小麦品种具有指导意义。
本研究以龙葵为试验材料, 采用水培方法分析了补充Fe对龙葵幼苗Zn毒害耐受性的影响。结果表明, 补充200 ?mol·L<sup>-1</sup> Fe-EDTA提高了龙葵幼苗对Zn毒害的耐受性。400 ZnCl<sub>2</sub>处理导致龙葵幼苗株高、根长和叶绿素含量显著减少, 并引起H<sub>2</sub>O<sub>2</sub>的积累。补充Fe后, 植株生长状况得到明显改善, SOD、CAT和APX基因的表达和酶的活性显著提高。同时RT-PCR结果显示, 补充Fe后,<i> FeSOD2</i>和<i>CAT1</i>基因的表达明显升高。这些结果表明, Zn毒害对龙葵幼苗生长发育的影响很大程度上可能是由于Zn毒害引起的缺Fe导致的；Zn毒害条件下补充Fe可以通过提高<i>FeSOD2</i>和<i>CAT1</i>基因的表达, 提高抗氧化酶的活性, 降低ROS的水平, 降低植株的氧化伤害。本研究为进一步研究植物响应Zn毒害的生理分子机理, 以及植物修复技术的实际应用提供了理论基础。