A5100382787- Fire dynamics and safety research
- Plant Molecular Biology Research
- Combustion and Detonation Processes
- Combustion and flame dynamics
- Plant Stress Responses and Tolerance
- Flame retardant materials and properties
- Heat shock proteins research
- Plant-Microbe Interactions and Immunity
- Advanced Combustion Engine Technologies
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Monoclonal and Polyclonal Antibodies Research
- Connexins and lens biology
- Signaling Pathways in Disease
- NF-κB Signaling Pathways
- Plant Pathogenic Bacteria Studies
- Plant biochemistry and biosynthesis
- RNA Research and Splicing
- Nuclear Structure and Function
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Mechanisms of cancer metastasis
- Polysaccharides and Plant Cell Walls
- Insect symbiosis and bacterial influences
- Plant Parasitism and Resistance
Institute of Mechanics
2016-2023
Chinese Academy of Sciences
2013-2023
University of Chinese Academy of Sciences
2014-2023
Northeast Agricultural University
2023
Max Planck Institute of Molecular Plant Physiology
2022-2023
ShenZhen People’s Hospital
2022
Institute of Genetics and Developmental Biology
2013-2020
State Key Laboratory of Plant Genomics
2016-2020
Center for Agricultural Resources Research
2017-2020
Shenzhen University
2020
Abstract Generation of stable gene-edited plant lines using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy process outcrossing to eliminate CRISPR–Cas9-associated sequences and produce transgene-free lines. We have addressed this issue by designing fusions Cas9 guide RNA transcripts tRNA-like sequence motifs that move RNAs from transgenic rootstocks grafted wild-type shoots (scions) achieve heritable gene editing, as...
Group A protein type 2C phosphatases (PP2Cs) are negative regulators of abscisic acid (ABA) signalling and plant adaptation to stress. However, our knowledge the regulation PP2C activity is limited. Here we report that HAB1 undergoes alternative splicing produce two splice variants, which encode HAB1.1 HAB1.2, play opposing roles in ABA-mediated seed germination post-germination developmental arrest. HAB1.2 predominately formed presence ABA prevents post-germinative growth. interacts with...
Cell water permeability and cell wall properties are critical to survival of plant cells during freezing, however the underlying molecular mechanisms remain elusive. Here, we report that a specifically cold-induced nuclear protein, Tolerant Chilling Freezing 1 (TCF1), interacts with histones H3 H4 associates chromatin containing target gene, BLUE-COPPER-BINDING PROTEIN (BCB), encoding glycosylphosphatidylinositol-anchored protein regulates lignin biosynthesis. Loss TCF1 function leads...
Innate immunity plays a vital role in protecting plants and animals from pathogen infections. Immunity varies with age both plants. However, little is known about the ontogeny of plant innate during seedling development. We report here that Arabidopsis (Arabidopsis thaliana) microRNA miR172b regulates transcription immune receptor gene FLAGELLIN-SENSING2 (FLS2) through TARGET OF EAT1 (TOE1) TOE2, which directly bind to FLS2 promoter inhibit its activity. The level very low early stage...
The import of proteins into the nucleus in response to drought is critical for mediating reprogramming gene expression that leads tolerance. However, regulatory mechanisms involved nuclear protein remain largely unknown. Here, we have identified an Arabidopsis (AtKPNB1) as a homolog human KPNB1 (importin β1). AtKPNB1 was expressed multiple organs, and localized cytoplasm nucleus. able facilitate model protein. Null mutation delayed development under normal growth conditions increased...
The stem cell niche in the root meristem maintains pluripotent cells to ensure a constant supply of for growth. Despite extensive progress, molecular mechanisms through which fates and activity are determined remain largely unknown. In Arabidopsis thaliana, Pleiotropic Regulatory Locus 1 (PRL1) encodes WD40-repeat protein subunit spliceosome-activating Nineteen Complex (NTC) that plays role multiple stress, hormone developmental signaling pathways. Here, we show PRL1 is involved control size...
Receptor-like kinases (RLKs) play important roles in plant immunity signaling; thus, many are hijacked by pathogen effectors to promote successful pathogenesis. Xanthomonas oryzae pv. (Xoo) is the causal agent of rice leaf blight disease. The strain PXO99A has 18 non-TAL (transcription activation-like) effectors; however, their mechanisms action and host target proteins remain largely unknown. Although effector XopR from Xoo MAFF311018 was shown suppress PAMP-triggered immune responses...
Summary Abscisic acid‐insensitive 5 ( ABI 5) is an essential and conserved plant basic leucine zipper transcription factor whose level controls seed germination postgerminative development. It has been demonstrated that activity of transcriptionally post‐translationally regulated. However, transcriptional regulation not fully understood. Here, we identified SAB 1 Sensitive to ABA ) as a novel negative regulator simultaneously regulates its stability, promoter binding histone...
Arabidopsis AVRPPHB SUSCEPTIBLE1 (PBS1) serves as a "decoy" in activating RESISTANCE TO PSEUDOMONAS SYRINGAE5 (RPS5) upon cleavage by Pseudomonas phaseolicola B (AvrPphB), syringae effector. The SEMPH motif PBS1 was thought to allow it be distinguished RPS5 from the closely related kinases. However, underlying mechanism is not fully understood. Here, we isolated and characterized wheat homolog, TaPBS1. Although this plasma membrane-localized kinase could cleaved AvrPphB associate with RPS5,...
Summary The HSC70/HSP70 family of heat shock proteins are evolutionarily conserved chaperones involved in protein folding, transport, and RNA binding. Arabidopsis HSC70 thought to act as housekeeping such many growth‐related pathways. Whether binds whether this interaction is functional has remained an open question. We provide evidence that the HSC70.1 chaperone its own mRNA via C‐terminal short variable region (SVR) inhibits translation. SVR encoding necessary for transcript mobility...
The rapid development of the Internet, especially emergence social networks, has led rumor propagation into a new media era. In this paper, we are concerned with stochastic delayed model. Firstly, obtain existence global solution. Secondly, sufficient conditions for extinction established. Lastly, boundedness solution is proved and some simulations given to verify our results.
Summary The plant hormones brassinosteroids ( BR s) modulate growth and development. Cysteine (Cys) residues located in the extracellular domain of a protein are importance for structure by forming disulfide bonds. To date, systematic study functional significance Cys ‐insensitive 1 BRI 1) is still lacking. We used brassinolide‐induced exogenous bri1 ‐ EMS ‐Suppressor BES dephosphorylation Arabidopsis thaliana protoplasts as readout, took advantage dramatic decrease levels during protoplast...
Mutations in GJA8 are associated with hereditary autosomal dominant and recessive cataract formation. In this study, a novel insert mutation was identified Chinese congenital family cosegregated the disease pedigree. This introduces five additional amino acid residues YAVHY after histidine at 95 site (p.H95_A96insYAVHY) within second transmembrane (TM2) domain of Cx50 protein (Cx50-insert). Ectopic expression Cx50-insert impairs hemichannel functions gap junction activity compared to...
Heat shock factor 4 controls the transcription of small heat proteins (e.g., HSP25, alpha B-cyrstallin, and r-crystallin), that play important roles in modulating lens proteostasis. However, molecular mechanism underlying HSF4-mediated is still unclear. Using yeast two hybrid, we found HSF4 interacts with ATP-dependent DEXD/H-box RNA helicase UAP56, their interaction epithelial cell line was further confirmed by GST-pull down assay. UAP56 a vital regulator pre-mRNA splicing mature mRNA...
Melanoma is the most malignant form of skin cancer with high metastatic potential. Nuclear factor activated T-cells (NFATs) are discovered as transcription factors that regulate expression proinflammatory cytokines and other genes during immune response. Among five NFAT members, NFAT3 exclusively not expressed in cells its role progression different types remains controversial. Our previous study showed was highly compared normal tissues critical for melanoma cell survival tumor growth....
Type III interferon (IFN-λs) exhibit potent antiviral activity and immunomodulatory effects in specific cells. Nucleotide fragements of bovine ifn-λ (boifn-λ) gene was synthetized after codon optimization. The boifn-λ then amplified by splicing overlap extension PCR (SOE PCR), resulting serendipitous acquisition the mutated boIFN-λ3V18M. Recombinant plasmids pPICZαA-boIFN-λ3/λ3V18M were constructed corresponding proteins expressed Pichia pastoris with high-level extracellular soluble form....
Liver cancer stem cells (LCSCs) cause therapeutic refractoriness and relapse in hepatocellular carcinoma. Heat shock factor 1 (HSF1) plays versatile roles multiple cancers. However, the role of HSF1 LCSCs is not well understood. This study investigated function signal mechanisms maintaining LCSC phenotypes.We established two lines, HepG2-R HuH-7-R. Constitutive activation was observed these LCSCs. Specific short hairpin RNAs (shRNAs) chemical inhibitors were used to identify relationship...