A5022721555- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Proteoglycans and glycosaminoglycans research
- Bone Tissue Engineering Materials
- Silk-based biomaterials and applications
- Periodontal Regeneration and Treatments
- Dermatology and Skin Diseases
- Orthopaedic implants and arthroplasty
- Skin and Cellular Biology Research
- Pineapple and bromelain studies
- Gut microbiota and health
- Nanocomposite Films for Food Packaging
- Lipid Membrane Structure and Behavior
- Advanced Chemical Sensor Technologies
- Hemostasis and retained surgical items
- Cholesterol and Lipid Metabolism
- Dendrimers and Hyperbranched Polymers
- Global Energy Security and Policy
- Fatty Acid Research and Health
- NF-κB Signaling Pathways
- Mast cells and histamine
- Asthma and respiratory diseases
- Knee injuries and reconstruction techniques
- Hydrogels: synthesis, properties, applications
Tokyo Denki University
2010-2022
Kyocera (United States)
2001-2005
Kyocera (Japan)
2003-2004
Abstract Mutations in patatin-like phospholipase domain-containing 1 (PNPLA1) cause autosomal recessive congenital ichthyosis, but the mechanism involved remains unclear. Here we show that PNPLA1, an enzyme expressed differentiated keratinocytes, plays a crucial role biosynthesis of ω- O -acylceramide, lipid component essential for skin barrier. Global or keratinocyte-specific Pnpla1 -deficient neonates die due to epidermal permeability barrier defects with severe transepidermal water loss,...
Epidermal lipids are important for skin homeostasis. However, the entire picture of roles lipids, particularly nonceramide lipid species, in epidermal biology still remains obscure. Here, we report that PLA2G2F, a functionally orphan-secreted phospholipase A2 expressed suprabasal epidermis, regulates homeostasis and hyperplasic disorders. Pla2g2f−/− mice had fragile stratum corneum were strikingly protected from psoriasis, contact dermatitis, cancer. Conversely, Pla2g2f-overexpressing...
Phospholipase A2 enzymes have long been implicated in the promotion of inflammation by mobilizing pro-inflammatory lipid mediators, yet recent evidence suggests that they also contribute to anti-inflammatory or pro-resolving programs. Group IID-secreted phospholipase (sPLA2-IID) is abundantly expressed dendritic cells lymphoid tissues and resolves Th1 immune response controlling steady-state levels lipids such as docosahexaenoic acid its metabolites. Here, we show psoriasis contact...
Besides promoting inflammation by mobilizing lipid mediators, group IIA secreted phospholipase A2 (sPLA2-IIA) prevents bacterial infection degrading membranes. Here, we show that, despite the restricted intestinal expression of sPLA2-IIA in BALB/c mice, its genetic deletion leads to amelioration cancer and exacerbation psoriasis distal skin. Intestinal is reduced after treatment with antibiotics or under germ-free conditions, suggesting upregulation gut microbiota. Metagenome, transcriptome,...
We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in short treatment time. The aim of the study is investigate efficiency and its relation with ultrasonic power output dissolved oxygen (DO) concentration different detergent solution. In study, we used aorta samples evaluate efficiency, which assessed duration, SDS with/without saline. treated were evaluated histologically by Hematoxylin Eosin (HE) staining scanning electron...
Abstract Fibrin deposition and platelet adhesion onto alkali‐ heat‐treated titanium metal (AH‐Ti), water‐treated (Wa‐Ti), formed with apatite (Ap‐Ti) in simulated body fluid (SBF) were evaluated by exposure to anticoagulated blood or washed suspension (WPS) under static conditions subsequent observation scanning electron microscopy (SEM). The results compared those for commercially pure (cp‐Ti). Thrombus formation on AH‐Ti Wa‐Ti, which exposed heparinized whole 1 h, was significantly less...
A novel bioabsorbable bone substitute composed of a beta-tricalcium phosphate (beta-TCP) and carboxymethyl-chitin (CM-chitin) sodium has been developed. Rabbit tibia defects (4 mm in diameter) were repaired after 4 weeks more effectively by the composite compared with sham-operation group. To further investigate biological safety components, genotoxicity carcinogenicity an extract prepared from determined using four different vitro assays. The main component was identified as CM-chitin...
Novel poly(N-isopropylacrylamide-co-N-tert-butylacrylamide)-grafted hyaluronan [P(NIPAAm-co-NtBAAm)-g-HA] has been developed as a modified derivative to improve phase-transition characteristics of PNIPAAm-g-HA, which lower critical solution temperature (LCST) approximately 32°C. This promising self-assembling biomaterial potential an injectable scaffold for in situ cartilage tissue engineering. LCST the P(NIPAAm-co-NtBAAm)-g-HA decreased 3.6°C compared that original PNIPAAm-g-HA....
In order to contribute the development of minimally invasive surgery techniques for autologous chondrocyte implantation, a novel self-assembling biomaterial consisting thermoresponsive poly(N-isopropylacrylamide)-grafted hyaluronan (PNIPAAm-g-HA) has been synthesized as an injectable scaffold cartilage tissue engineering. The aim this study was investigate efficacy and cytocompatibility PNIPAAm-g-HA normal chondrocytes by using reverse transcription-polymerase chain reaction (RT-PCR)...
Abstract A novel hyaluronan (HA) derivative, poly(L‐glutamic acid)‐grafted (PGA‐g‐HA), was synthesized to improve the durability of conventional HA products for intra‐articular injection. The purpose this study investigate characteristics derivative in terms viscoelasticity, degradation behavior, non‐immunogenicity, and bioactivity using preliminary vitro vivo experiments. storage modulus ( G ′) loss G″ ) PGA‐g‐HA were similar those HA80 (approximately 8.0 × 10 5 Da) rather than original...
The aim of the present study was to compare acute and cumulative cytotoxicity intact (n-GE) warmed genipin (w-GE), while investigating differences in crosslinking capabilities these two genipins by rheological mechanical tests. n-GE solution prepared dissolving powder a sodium phosphate buffer solution. w-GE warming at 37 °C for 24 h. tests chitosan (CH)/genipin gels showed rate much greater than that up until 6 h after preparation, whereas degree CH/n-GE became higher 12 ISO 10993-5...
The blood-contacting surface, mainly composed of polycarbonate, the Gyro C1E3 pump was modified using plasma glow discharge to introduce a carboxyl functional group, coated with base layer polyethyleneimine as linker, and coupled heparin by multi-ionic binding enhance blood compatibility. A relative surface content 3.7% sulfur, which demonstrated immobilization on polycarbonate substrate, observed x-ray photoelectron spectroscopy, initial bioactivity approximately 88.5 +/- 7.3 mIU/cm2...
Beta-tricalcium phosphate/carboxymethyl chitin composites [TCP/CMCh] of various ratios TCP granules and CMCh were made their mechanical properties, handling properties repair performance for bone defects osteochondral investigated. Water pooling ratio was approximately 40 times the weight itself. TCP/CMCh a higher had stress at 50%-strain. The 50%-strain with 0, 2.5, 5.0, 7.5, 10 0.12, 0.51, 1.08, 1.46, 1.67 (MPa, n=5), respectively. 5.0 best total scores in tests. rate 2.5< Blank=...
OBJECTS: Hydrogen has been shown to possess antibacterial effects at high concentrations. In addition, chlorine a strong bactericidal effect even low Electrolysis is way simultaneously generate these two components. However, the concentration of hydrogen/free mixed water generated through electrolysis decreases quickly. It predicted that will vary depending on quality used. After investigating optimum generation conditions, most stable carious tooth fungus were evaluated in vitro. Thus, this...