A5082774070- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Immune cells in cancer
- Tryptophan and brain disorders
- Mathematical Biology Tumor Growth
- Neuroendocrine regulation and behavior
- Histone Deacetylase Inhibitors Research
- Cell Image Analysis Techniques
- Nuclear Receptors and Signaling
- Advanced Fluorescence Microscopy Techniques
- Memory and Neural Mechanisms
- Glioma Diagnosis and Treatment
- Chemokine receptors and signaling
- Computational Drug Discovery Methods
University Hospital Bonn
2024
German Center for Neurodegenerative Diseases
2014-2024
Microglia, the resident immune cells of brain, play a complex role in health and disease. They actively survey brain parenchyma by physically interacting with other structurally shaping brain. Yet, mechanisms underlying microglial motility significance for synapse stability, especially hippocampus during adulthood, remain widely unresolved. Here, we investigated effect neuronal activity on implications formation survival dendritic spines hippocampal CA1 neurons vivo. We used repetitive...
Hippocampal function is important for learning and memory. During memory processing, hippocampal CA1 neurons play a crucial role by integrating excitatory synaptic input from CA3 the entorhinal cortex. These receive almost exclusively on dendritic spines. The formation elimination—structural plasticity—of spines reflect wiring changes within network. Despite relevance of hippocampus in memory, most vivo data structural plasticity derive cortical regions. We established chronic window...
Abstract The medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical optical approaches. Here we apply low three-photon imaging mouse at unprecedented depth. Specifically, measure astrocytic Ca 2+ -transient parameters awake head-fixed mice up to a depth 1700 µm. Furthermore, longitudinally record dendritic spine...
Abstract Microglia, the resident immune cells of brain, play a complex role in health and disease. They actively survey brain parenchyma by physically interacting with other structurally shaping brain. Yet, mechanisms underlying microglia motility their significance for synapse stability, especially during adulthood, remain widely unresolved. Here we investigated impact neuronal activity on its implication formation survival. We used repetitive two-photon vivo imaging hippocampus awake mice...
The interaction of glioblastoma (GB) and microglia is critical due to its implications for tumor progression, immune response modulation, potential therapeutic strategies. However, the role in GB pathogenesis remains unclear, especially regarding vivo dynamics their interplay. Performing three-photon imaging an autochthonous, immunocompetent mouse model, we examined tumor/microglia within previously inaccessible regions at far infiltration zone corpus callosum. Initially, increased tissue...
Microglia are highly motile cells that play a crucial role in the central nervous system health and disease. Here we show actin depolymerizing factors ADF Cofilin1 (Cfl1) key of microglia integrity function. We found profound morphological phenotype absence Cfl1 microglia. In vivo two-photon imaging with ADF/Cfl1-KO revealed reduced microglial fine processes motility impaired migration towards laser-induced lesion. increased accumulation stabilized F-actin altered microtubule dynamics...
Abstract BACKGROUND Glioblastomas are the most malignant primary brain tumors with median survival of about 17 month after diagnosis despite extensive treatment. Molecular heterogeneity and tumor microenvironment contribute to challenges in treating GBs. Glioma-associated microglia macrophages, comprising 30-50% non-neoplastic cells microenvironment, play a crucial role facilitating growth inducing tissue necrosis. However, intricate interplay between these immune glioma remains poorly...
Abstract Accumulating evidence supports immune involvement in the pathogenesis of schizophrenia, a severe psychiatric disorder. In particular, high expression variants C4 , gene innate complement system, were shown to confer susceptibility schizophrenia. However, how elevated may impact brain circuits remains largely unknown. We used utero electroporation overexpress mouse prefrontal cortex (PFC). found reduced glutamatergic input pyramidal cells juvenile and adult, but not newborn...