A5057565467- HIV Research and Treatment
- Tuberculosis Research and Epidemiology
- Immune Cell Function and Interaction
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- vaccines and immunoinformatics approaches
- Cytomegalovirus and herpesvirus research
- RNA and protein synthesis mechanisms
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- Diagnosis and treatment of tuberculosis
- RNA modifications and cancer
- Animal health and immunology
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Research on Leishmaniasis Studies
- Genomics and Chromatin Dynamics
- Trypanosoma species research and implications
- Epigenetics and DNA Methylation
- Iron Metabolism and Disorders
Cornell University
2019-2025
Weill Cornell Medicine
2020-2024
New York University
2016-2018
Fundação Oswaldo Cruz
2016
Abstract Human challenge experiments could accelerate tuberculosis vaccine development. This requires a safe Mycobacterium (Mtb) strain that can both replicate in the host and be reliably cleared. Here we genetically engineered Mtb strains encoding up to three kill switches: two mycobacteriophage lysin operons negatively regulated by tetracycline degron domain–NadE fusion, which induces ClpC1-dependent degradation of essential enzyme NadE, trimethoprim. The triple-kill-switch (TKS) showed...
Visceral leishmaniasis (VL) is a disease caused by Leishmania infantum, which transmitted phlebotomine sandflies. Dogs are the main urban reservoir of this parasite and presents similar characteristics in both humans dogs. In paper, we investigated potential pathways involved plasma cell replacement normal populations spleen, with respect to severity dogs from an endemic area for visceral leishmaniasis. To end, canine spleen samples were grouped into three categories: TYPE1SC- (non-infected...
HIV persists, despite immune responses and antiretroviral therapy, in viral reservoirs that seed rebound viremia if therapy is interrupted. Previously, we showed the BCL-2 protein contributes to persistence by conferring a survival advantage reservoir-harboring cells. Here, demonstrate many of family members are overexpressed HIV-infected CD4+ T cells, indicating increased tension between proapoptotic prosurvival members-and suggesting inhibition may disproportionately affect Based on these...
Abstract Human challenge experiments could greatly accelerate the development of a tuberculosis (TB) vaccine. for requires strain that can both replicate in host and be reliably cleared. To accomplish this, we designed Mycobacterium (Mtb) strains featuring up to three orthogonal kill switches, tightly regulated by exogenous tetracyclines trimethoprim. The resultant displayed immunogenicity antibiotic susceptibility similar wild-type Mtb under permissive conditions. In absence supplementary...
Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, poses a great threat to human health. With emergence drug resistant Mtb strains, new therapeutics are desperately needed. As iron is critical growth and survival Mtb, mechanisms through which acquires host represent attractive therapeutic targets. scavenges via siderophore-dependent heme uptake pathways. While multiple studies describe import ferric-siderophores export apo-siderophores across inner membrane, little known...
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Abstract Persistent HIV reservoirs in CD4⁺ T-cells pose a barrier to curing infection. We identified overexpression of enhancer zeste homolog 2 (EZH2) HIV-infected T- cells that survive cytotoxic T lymphocyte (CTL) exposure, suggesting mechanism CTL resistance. Inhibition EZH2 with the FDA-approved drug tazemetostat increased surface expression major histocompatibility complex class I (MHC-I) on T-cells, counterbalancing Nef–mediated MHC-I downregulation. This improved CTL-mediated...
Abstract HIV persists, despite antiviral immune responses and effective antiretroviral therapy, in viral reservoirs that seed rebound viremia if therapy is interrupted. Previously, we showed the BCL-2 protein contributes to persistence by conferring a survival advantage reservoir-harboring cells. Here, demonstrate many of family members are overexpressed HIV-infected CD4 + T-cells, indicating increased tension between pro-apoptotic pro-survival – as well raising possibility inhibition may...