There is an increasing interest in non-destructive and real-time high-resolution approaches for corrosion studies metals. In this paper, we propose the dynamic speckle pattern method as a low-cost, easy-to-implement, quasi in-situ optical technique quantitative evaluation of pitting corrosion. This type occurs specific area metallic structure causes holes formation leading to structural failure. A Custom 450 stainless steel sample, placed 3.5 wt% NaCl solution applied [Formula: see text]...

Purpose: Digital holographic microscopy, being a label-free and entirely non-invasive technique, proficiently facilitates the quantitative assessment of morphological alterations in living cells. The primary objective this investigation is to scrutinize capability digital microscope assessing transformations cancer cells exposed X-ray radiation. Materials Methods: Michigan Cancer Foundation-7 (MCF7) cell line underwent exposure radiation, administered singular fraction at dose 2 Gy....

This paper presents a robust approach to polarimetric microscopy by integrating microsphere-assisted microscopy, dynamic laser speckle analysis (DLSA), and imaging. DLSA creatively transforms speckle-induced imaging challenges into opportunities, while simultaneously overcoming traditional polarization interpretation difficulties analyzing sequence-based variations in pattern, caused the sample’s responses during full measurement. The inclusion of dielectric microsphere (MS) significantly...

Introduction: Doxorubicin (DOX ) is a widely used first-line treatment for various cancers but causes toxicity. Targeted drug delivery systems, particularly DOX-encapsulated liposomes, show clinical success and lower The abnormal angiogenesis in high-grade tumors, making it crucial to develop strategies that target this process conjunction with chemotherapy. This study presents an innovative formulation of anti-VEGFR2-functionalized liposomal DOX, designed reduce systemic release, enhance...

Monitoring pitting corrosion in real-time and non-destructively is a critical challenge material science. This study introduces dynamic speckle analysis as an innovative approach to quantitatively track metallic structures. Pitting corrosion, major cause of structural failure due hole formation, effectively visualized using this technique. Dynamic patterns, generated by laser light scattering off the corroded surface, reveal both activity progression corrosion. We employ range graphical...

Scaffold-based bone tissue engineering aims to develop 3D scaffolds that mimic the extracellular matrix regenerate defects and damages. In this paper, we provide a laser speckle analysis characterize highly porous scaffold. The experimental procedure includes in situ acquisition of patterns scaffold at different times under preserved environmental conditions, follow-up statistical post-processing toward examining its internal activity. activity overall viscoelastic properties are expressed...

Investigating real-time phenomena in bio-polymers has received much attention because of their increasing demands polymer substitution. The 3D morphometry surfaces may be very impactful such studies. Here, digital holographic microscopy (DHM) is applied for quantitative measurement the rare morphological changes UV-A and UV-C exposed nanocomposites during incubation with excess water. By reconstructing recorded successive holograms, time evolution swelled regions samples derived. Our results...

Multicomponent mixtures of bilayer lipids, thanks to the coexistence liquid-crystalline phases in their structures, may be used development functional membranes. In such membranes interlayer ordering distributes across membrane lamellae, resulting long-range alignment phase-separated domains. this paper, we explore dynamics phenomenon by laser speckle pattern analysis. We show that cholesterol content decreases activity, and rate domains size is related change physical roughness...

In this paper, we show that laser speckle analysis (LSA) can provide valuable information about the structure of crumpled thin sheets. Crumpling and folding slender objects are present in several phenomena various ranges size, e.g., paper compaction, cortical brains, DNA packing viral capsids, flower buds, to name a few. The speckles, both numerical graphical, is source activity biological or non-biological materials, development digital electronics, which brought ease image processing, has...

Abstract In several phenomena in biology and industry, it is required to understand the comprehensive behavior of sedimenting micro-particles fluids. Here, we use numerical refocusing feature digital holographic microscopy (DHM) investigate slippage effect on micro-particle sedimentation near a flat wall. DHM provides quantitative phase contrast three-dimensional (3D) imaging arbitrary time scales, which suggests as an elegant approach various phenomena, including dynamic colloids. 3D...

Abstract We investigate the sedimentation of colloidal micro-spheres and red blood cells (RBCs) in non-Newtonian fluid - silicone oil with different viscosities. use digital holographic microscopy (DHM) to obtain volumetric information sedimenting micro-objects. Especially, numerical refocusing feature DHM is used extract depth multiple particles moving inside fluid. The effects proximity a flat wall behavior on RBCs are studied by trajectory analyzing velocimetry. observe that for lower...

The calcium level in blood affects the morphological and rheological properties of red cell (RBC) membranes. In this paper, we present an integrated optical system for a single study hypercalcemia. consists holographic tweezers blinking tweezers, photo-damage-free immobilization cells, combined with digital microscopy, quantitative analysis live visualization cells. Digital holograms were recorded live, while concentration ions buffer is gradually increased. Full morphometric data RBCs...

Definite diagnosis of brain neoplasms has been a challenge neurosurgery practice especially when open surgery is not feasible to obtain adequate tissue samples for pathological investigation (e.g., deep-seated tumors or eloquent areas undergoing biopsies recurrent cases in patients that are candidates palliative care). This complexity exacerbated due tumor heterogeneity, particularly relying on limited obtained through stereotactic biopsies, which increases the risk misdiagnosis. Addressing...

Spiropyran (SP)-based dynamic materials undergo structural changes in response to external stimuli. In this paper, we show that digital holographic microscopy (DHM) is an effective candidate for characterisation of SPs (embedded polymer matrices) and monitoring their dynamical changes. The matrices are polylactic acid (PLA) poly(methyl methacrylate) (PMMA) films, which decorated with immobilised on graphene quantum dots (GQDs). GQDs modified by benzylamines prior the loading SP species...