Introduction: Recently, the focus of oncological research has been on optimization therapeutic strategies targeted at malignant diseases. Nanomedicine utilizing silicon dioxide nanoparticles (SiNPs) is one such strategy and rapidly developing as a promising tool for cancer diagnosis, imaging, treatment. Nevertheless, little known about mechanisms action SiNPs in brain tumors. Materials methods: Here, we explored effects 5–15 nm human glioblastoma cell line LN229. In this respect, MTT assays,...

Silica nanoparticles (SiNPs) are one of the most commonly used nanomaterials in various medical applications. However, possible mechanisms toxicity caused by SiNPs remain unclear. The study presented here provides novel information on molecular and cellular effects glioblastoma LBC3 LN-18 cells. It has been demonstrated that 7 nm, 5-15 nm 10-20 induce time- dose-dependent cytotoxicity cell lines. In contrast to cells, we observed only weak reduction viability normal skin fibroblasts treated...

A growing body of evidence indicates that dietary polyphenols show protective effects against various cancers. However, little is known yet about their activity in brain tumors. Here we investigated the interaction flavonoid quercetin (QCT) with human glioblastoma A172 and LBC3 cell lines. We demonstrated QCT evoked cytotoxic effect both tested Microscopic observations, Annexin V-FITC/PI staining, elevated expression caspase 3/7 showed caused predominantly apoptotic death cells. Further...

Phenylbutyrate (PBA) is a histone deacetylase inhibitor known for inducing differentiation, cell cycle arrest, and apoptosis in various cancer cells. However, the effects of PBA seem to be very cell-type-specific sometimes limited exclusively particular line. Here, we provided novel information concerning cellular LN-229 LN-18 glioblastoma lines which have not been previously evaluated context exposure. We found that cells were PBA-insensitive even at high concentrations PBA. In contrary,...

Skin is the largest organ in human body, and which protects organism against unfavorable external factors e.g., chemicals, environment pollutants, allergens, microorganisms, it plays a crucial role maintaining general homeostasis. It also an important target of oxidative stress due to activity oxygen reactive species (ROS), are constantly generated fibroblasts response exogenous or endogenous prooxidant agents. An example such compound with proved Doxorubicin (DOX), effective anticancer...

Adherent interactions between integrins and extracellular matrix (ECM) proteins play an important role in tumorigenicity invasiveness. The major component of ECM is collagen that plays a central the interaction with integrins. expression certain collagenases (gelatinases) by tumour cells one characteristic features so-called metastatic phenotype, presumably breaking down barriers as well altering ECM-cell interaction. Although initiate breakdown collagen, final step degradation catalysed...

Deregulated PI3K/AKT/mTOR signalling commonly exists in glioblastoma, making this axis an attractive target for therapeutic manipulation. Given that activation of promotes tumour growth, metastasis, and resistance to anticancer therapies, mTOR inhibitors show promise the treatment cancer. The aim study was investigate underlying mechanism novel dual PI3K/mTOR inhibitor, Apitolisib (GDC-0980), A-172 U-118-MG GBM cell line suppression. It has been demonstrated GDC-0980 induces time-...

The collagen metabolism alterations triggered by reactive oxygen species are involved in the development of various connective tissue diseases and skin aging. This study was designed to examine whether (E)-anethole possesses a protective effect on H2O2-induced as well it can prevent apoptosis human fibroblasts. In cells treated with 300 µM H₂O₂, decrease biosynthesis 54% observed. Pretreatment 0.5 anethole for 1 h completely prevented this alteration. Changes at protein level positively...

Cancer cells have developed a number of adaptation mechanisms involving the signal activation transduction pathways, which promotes progression and metastasis. Our results showed that percentage apoptotic MCF-7 incubated in low glucose medium for 48 h was lower comparison to those cultured high medium, despite expression proapoptotic transcription factor—CHOP. Furthermore, higher NF-κB p100/p52 subunits compared medium. Moreover, our findings demonstrated shortage strongly induces autophagy...

Reduced graphene oxide (rGO) has already been reported as a potential cytostatic agent in various cancers. However, the mechanisms underlying rGO's cytotoxicity are still insufficiently understood. Thus, aim of study was to investigate molecular and cellular effects rGO breast cancer. Given this, two cell lines, MDA-MB-231 ZR-75-1, were analyzed using MTT test, flow cytometry Western blot assay. Incubation with resulted multitude effects, including stimulation autophagy, cycle arrest and,...

Mesotrione, as a widely used herbicide, is present in the environment detectable amounts, causing serious damage. Here, we aimed to investigate effect of mesotrione on Caco-2 cells and possibility its toxicity mitigation by cichoric acid. Therefore, analyzed cytotoxicity both these compounds selected oxidative stress parameters, apoptosis interaction tested with cell membrane their accumulation within cells. In studies, stimulating activity was observed, simultaneously, inhibitory acid...

Histone deacetylase (HDAC) inhibitors are now intensively investigated as potential cytostatic agents in many malignancies. Here, we provide novel information concerning the influence of belinostat (Bel), a hydroxamate-based pan-HDAC inhibitor, on glioblastoma LN-229 and LN-18 cells. We found that cells stimulated with 2 μmol/L Bel for 48 h resulted 70 % apoptosis, while equivalent treatment only 28 apoptosis. In this effect was followed by up-regulation pro-apoptotic genes including Puma,...

Malignant melanoma-a tumor originating from melanocytes-is characterized by dynamic growth and frequent metastases in the early stage of development. Current therapy methods are still insufficient, there is a need to search for new ways treating this malady. The induction apoptosis-physiological cell death-by proteasome inhibitors recognized as an effective method non-invasive elimination cancer cells. In our research, we wanted check potential marizomib (MZB, salinosporamide A, NPI-0052)-an...

Proteasomes play an important role in the physiology of cancer cells, and inhibition their activity may be used as a promising therapeutic strategy against glioblastoma (GBM). Although certain proteasome inhibitors (PIs) have been approved for treatment other malignancies, they limited effectiveness GBM due to low brain bioavailability. Marizomib (MZB) is irreversible, second-generation inhibitor, which unlike PIs can penetrate through blood–brain barrier, making it tool malignancies. The...

Breast cancer is the most common diagnosed in women, however traditional therapies have several side effects. This has led to an urgent need explore novel drug approaches treatment strategies such as graphene-based nanomaterials reduced graphene oxide (rGO). It was noticed a potential due its target selectivity, easy functionalisation, chemisensitisation, and high drug-loading capacity. rGO widely used many fields, including biological biomedical, unique physicochemical properties. However,...

The aim of the present study was to assess profiles exoglycosidases: N-acetyl-β-hexosoaminidase, β glucuronidase and galactosidase, α mannosidase fucosidase in fibroblast culture undergoing replicative stress-induced senescence. Half cell grown normal conditions, without stressor, other half treated with 0.15 mM tert-butylhydroperoxide. activities total N-acetyl-β-hexosoaminidase as well lysate were determined duplicates using method Marciniak et al. Chatteriee modification by Zwierz...

The inhibition of the 26S proteasome evokes endoplasmic reticulum stress, which has been shown to be implicated in antitumoral effects inhibitors. cellular and molecular inhibitor-bortezomib-on human colon cancer cells are as yet poorly characterized. Bortezomib selectively induces apoptosis some cells. However, nature its selectivity remains unknown. Previously, we demonstrated that, contrast normal fibroblasts, bortezomib treatment evoked strong effect on breast incubated hypoxic normoxic...

The cellular and molecular effects of the proteasome inhibitor-bortezomib-on breast cancer cells are as yet poorly characterised. Bortezomib selectively induces apoptosis in some cells. However, nature its selectivity remains unknown. Previously, we demonstrated that: there was no effect bortezomib action on a time-dependent increase senescence human skin fibroblasts. study presented here provides novel information line MDA-MB-231. Our findings that contrast to normal fibroblasts, treatment...

Glucose deprivation is a factor evoking endoplasmic reticulum (ER) stress and induction of expression an oxygen-regulated protein 150 kDa (ORP150). We studied the effect inducible overexpression ORP150 on senescence apoptosis human breast carcinoma cells (MCF7) skin fibroblasts. found inhibitory MCF7 cells, but not fibroblasts in ER conditions. An increased senescence-associated β-galactosidase acid activity (biomarkers cellular senescence) was observed. suggest that cancer can promote...