A5073282365- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Electrohydrodynamics and Fluid Dynamics
- CRISPR and Genetic Engineering
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microbial metabolism and enzyme function
- Adipose Tissue and Metabolism
- Amino Acid Enzymes and Metabolism
- HIV/AIDS drug development and treatment
- Chemical Reactions and Isotopes
- Endoplasmic Reticulum Stress and Disease
- Metabolism, Diabetes, and Cancer
- Nanomaterials for catalytic reactions
- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- Plant-Derived Bioactive Compounds
- Cancer Research and Treatments
- Peroxisome Proliferator-Activated Receptors
- Bacterial Genetics and Biotechnology
- Microbial bioremediation and biosurfactants
- Microplastics and Plastic Pollution
- Monoclonal and Polyclonal Antibodies Research
Tsinghua University
2016-2021
Korea Advanced Institute of Science and Technology
2020-2021
State Key Laboratory of Chemical Engineering
2017-2018
Yangtze University
2015
Purdue University West Lafayette
2009-2011
Center for Cancer Research
2011
Fudan University
2010
ABSTRACT Surfactin‐family lipopeptides are green biosurfactants with substantial industrial potential. The major problem prohibiting surfactin use is the low titer of wild producer, Bacillus subtilis . Using transcriptomic analysis, four strong promoters, P groE , cdd rplK and sspE were identified cloned from genome B. THY‐7, a novel producer that has been soil 0.55 g/L titer. An optimal promoter, was selected to replace native THY‐7 synthase (SrfA) promoter through single‐cross homologous...
We report the design and synthesis of novel FTPA-triazole compounds as potent inhibitors isoprenylcysteine carboxyl methyltransferase (Icmt), through a focus on thioether isoprenoid mimetics. These mimetics were coupled utilizing copper-assisted cycloaddition to assemble potential inhibitors. Using resulting triazole from coupling an isoprenyl mimetic resulted in biphenyl substituted FTPA 10n. This lipid-modified analog is inhibitor Icmt (IC(50) = 0.8 ± 0.1 μM; calculated K(i) 0.4 μM).
A new type of three-dimensional (3D) serpentine microchannel reactor was fabricated by thermally bonding stacked polyimide films to greatly improve the mixing efficiency transversal vortices at low Reynolds number (Re). The effects Re, flow ratio R, and channel width on performance were investigated using Villermaux–Dushman method, in which segregation index XS utilized quantify efficiency. results showed that decreased with increasing decreasing reducing width. Then, a computational fluid...
Proteins require proper conformational energetics to fold and function correctly. Despite the importance of having information on energetics, investigation thermodynamic stability has been limited proteins, which can be easily expressed purified. Many biologically important proteins are not suitable for conventional biophysical because difficulty expression purification. As an effort overcome this limitation, we have developed a method determine low abundant in cell lysates. Previously, it...
Genome engineering of Rhodococcus opacus PD630, an important microorganism used for the bioconversion lignin, is currently dependent on inefficient homologous recombination. Although a CRISPR interference procedure gene repression has previously been developed R. CRISPR/Cas9 system knockout yet to be reported strain. In this study, we found that cytotoxicity Cas9 and deficiency in pathways repairing DNA double-strand breaks (DSBs) were major causes failure conventional technologies opacus,...
For large-scale bioproduction, thermal stability is a crucial property for most industrial enzymes. A new method to improve both the and activity of enzymes great significance. In this work, novel chaperones
The objective of this study was to compare the expression adenosine monophosphate-activated protein kinase ( AMPK) and its relation rates lipogenesis in muscle fast (Arbor Acres, AA chickens)-and slow (Bei-Jing-You, BJY chickens)-growing chicken strains. Twelve birds for each strain were selected slaughtered at 8 weeks age chickens week 8, 16 chickens. amount intramuscular fat (IMF) content, AMPK subunits key regulators thigh breast tissue determined. Results showed that content IMF...