A5025619811- Bacterial Genetics and Biotechnology
- Micro and Nano Robotics
- Advanced Electron Microscopy Techniques and Applications
- Bacteriophages and microbial interactions
- Force Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Microfluidic and Bio-sensing Technologies
- Origins and Evolution of Life
- Mitochondrial Function and Pathology
- Microtubule and mitosis dynamics
- Algal biology and biofuel production
- Cellular transport and secretion
- Nanopore and Nanochannel Transport Studies
- Bacterial biofilms and quorum sensing
- Quantum-Dot Cellular Automata
- DNA and Nucleic Acid Chemistry
- Microfluidic and Capillary Electrophoresis Applications
- Magnetic and Electromagnetic Effects
- Orbital Angular Momentum in Optics
- Mass Spectrometry Techniques and Applications
Chuo University
2019-2023
Osaka University
2010-2020
Ube Frontier University
2012-2014
Toyo University
2005-2010
Journal Article CryoTEM with a Cold Field Emission Gun That Moves Structural Biology into New Stage Get access Takayuki Kato, Kato Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan Search for other works by this author on: Oxford Academic Google Scholar Fumiaki Makino, Makino Takanori Nakane, Nakane MRC Laboratory Molecular Biology, Cambridge, UK Naoya Terahara, Terahara Takeshi Kaneko, Kaneko JEOL, Akishima, Tokyo, Yuko Shimizu, Shimizu Sohei Motoki, Motoki...
The bacterial flagellum is a motility organelle consisting of long helical filament as propeller and rotary motor that drives rapid rotation to produce thrust. Salmonella enterica serovar Typhimurium has two genes flagellin, fljB fliC, for flagellar formation autonomously switches their expression at frequency 10−3–10−4 per cell generation. We report here differences in structures functions under high-viscosity conditions. A strain expressing FljB showed higher than one FliC high viscosity....
In bacteria, the sodium ion (Na+) cycle plays a critical role in negotiating challenges of an extremely alkaline and sodium-rich environment. Alkaliphilic bacteria that grow optimally at high pH values use Na+ for solute uptake flagellar rotation because proton (H+) motive force is insufficient pH. Only three types electrically driven rotary motors exist nature: F-type ATPase, V-type bacterial motor. Until now, only H+ have been reported as coupling ions these motors. Here, we report...
Cooperative remodeling of the FlhA ring terminates hook assembly and initiates filament at tip.
We visualized the activation process of MotPS complex Bacillus flagellar motor by high-speed atomic force microscopy.
Bacterial flagella contain membrane-embedded stators, Mot complexes, that harness the energy of either transmembrane proton or sodium ion gradients to power motility. Use is associated with elevated pH and concentrations. The complexes studied date channels use protons ions, some bacteria having only one type others two distinct types different ion-coupling. Here, alkaliphilic Bacillus clausii KSM-K16 was shown be motile in a range from 7 11 although its genome encodes (BCl-MotAB). Assays...
Abstract Rotation of bacterial flagellar motor is driven by the interaction between stator and rotor driving energy supplied ion influx through channel. The composed MotA MotB proteins, which form a hetero-hexameric complex with stoichiometry four two molecules. are four- single-transmembrane respectively. To generate torque, MotA/MotB unit changes its conformation in response to interacts protein FliG. Here, we overproduced purified hyperthermophilic bacterium Aquifex aeolicus . A chemical...
Abstract The flagellar motor of Bacillus subtilis possesses two distinct H + -type MotAB and Na MotPS stators. In contrast to the motor, functions efficiently at elevated viscosity in presence 200 mM NaCl. Here, we analyzed torque-speed relationship motors over a wide range external loads. stall torque high load was about 2,200 pN nm 220 nm, respectively. number active stators estimated be ten one, However, functional increased up with an increase concentration polysaccharide, Ficoll 400, as...
The flagellar motor can spin in both counterclockwise (CCW) and clockwise (CW) directions. consists of a rotor multiple stator units, which act as proton channel. is composed the transmembrane MS ring made FliF cytoplasmic C consisting FliG, FliM, FliN. directly involved rotation directional switching. Salmonella FliF-FliG deletion fusion missing 56 residues from terminus 94 N FliG keeps domain responsible for interaction with intact, but its function reduced significantly. Here, we report...
The proton-driven flagellar motor of Salmonella enterica can accommodate a dozen MotA/B stators in load-dependent manner. C-terminal periplasmic domain MotB acts as structural switch to regulate the number active response load change. cytoplasmic loop termed MotAC is responsible for interaction with rotor protein, FliG. Here, test if stator assembly around manner, we analyzed effect mutations, M76V, L78W, Y83C, Y83H, I126F, R131L, A145E and E155K, on performance over wide range external...
A stem-loop mutation between ccpA and motP in the Bacillus subtilis ccpA-motPS operon increased motPS transcription membrane-associated MotPS levels, motility, number of flagella/cell when is sole stator contribution to motility at high pH, Na+, viscosity MotAB also present.
Abstract Recently, the structural analysis of protein complexes by cryo-electron microscopy (cryo-EM) single particle (SPA) has had great impact as a biophysical method. Many results cryo-EM SPA are based on data acquired state-of-the-art microscopes customized for SPA. These currently only available in limited locations around world, where securing machine time is highly competitive. One potential solution this time-competitive situation to reuse existing multi-purpose equipment, although...
The bacterial flagellar motor is a reversible rotary molecular nanomachine, which couples ion flux across the cytoplasmic membrane to torque generation. It comprises rotor and multiple stator complexes, each complex functions as an channel determines specificity of motor. Although coupling ions for rotation were presumed be only monovalent cations, such H+ Na+, MotA1/MotB1 Paenibacillus sp. TCA20 (MotA1TCA/MotB1TCA) was reported use divalent cations ions, Ca2+ Mg2+. In this study, we...
Abstract Bacillus subtilis possesses two distinct types of stator protein complexes for the flagellar motor: H + -type MotAB and Na MotPS. The MotPS complex is used when both external concentration viscosity are high. Because deletion motPS genes does not affect swimming motility cells, a physiological role MotPS-dependent remains unclear. Here, we report that required efficient biofilm maturation. Depletion did cause significant delay in initiation formation but reduced number viable cells...
The bacterial flagellum is a motility organelle, consisting of long helical filament as propeller and rotary motor that drives rapid rotation to produce thrust. Salmonella enterica serovar Typhimurium has two genes flagellin, fljB fliC, for flagellar formation autonomously switches their expression at frequency 10-3–10-4 per cell generation. We report here differences in structures functions under high viscosity conditions. A strain expressing FljB showed higher than the one FliC...