A5064391200- Advanced Electron Microscopy Techniques and Applications
- Astrophysical Phenomena and Observations
- Bacteriophages and microbial interactions
- Electron and X-Ray Spectroscopy Techniques
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Microtubule and mitosis dynamics
- Astrophysics and Cosmic Phenomena
- Particle Accelerators and Free-Electron Lasers
- Electrochemical sensors and biosensors
- Metalloenzymes and iron-sulfur proteins
- Gamma-ray bursts and supernovae
- Particle Detector Development and Performance
- Escherichia coli research studies
- Astronomical Observations and Instrumentation
- Microbial metabolism and enzyme function
- Chromosomal and Genetic Variations
- Superconducting Materials and Applications
- Microbial Fuel Cells and Bioremediation
- Cell Image Analysis Techniques
- Nuclear Physics and Applications
- Advanced X-ray Imaging Techniques
- CO2 Reduction Techniques and Catalysts
- DNA Repair Mechanisms
Osaka University
2016-2025
Toho University
2025
University of Osijek
2024
JEOL (Japan)
2019-2024
Ube Frontier University
2009-2023
University of Tsukuba
2022
Musashino University
2020
University of Bristol
2014-2016
Frontier Science Foundation
2016
France Business School
2016
The flagellar MS-ring is the initial template for assembly and houses protein export complex. has three parts of different symmetries within ring structure by FliF subunits in two conformations with distinct arrangements ring-building motifs, RBM1, RBM2, RBM3. However, it remains unknown how these are generated. A combination cryoEM structure-based mutational analyses demonstrates that well-conserved DQxGxxL motif RBM2-RBM3 hinge loop allows RBM2 to take orientations relative Of 34 basal...
Abstract We are amid the historic coronavirus infectious disease 2019 (COVID-19) pandemic. Imbalances in accessibility of vaccines, medicines, and diagnostics among countries, regions, populations, those war crises, have been problematic. Nanobodies small, stable, customizable, inexpensive to produce. Herein, we present a panel nanobodies that can detect spike proteins five SARS-CoV-2 variants concern (VOCs) including Omicron. Here show via ELISA, lateral flow, kinetic, flow cytometric,...
The bacterial flagellar type III export apparatus, which is required for assembly beyond the cell membranes, consists of a transmembrane gate complex and cytoplasmic ATPase complex. FlhA, FlhB, FliP, FliQ, FliR form inside basal body MS ring, although FliO efficient formation in Salmonella enterica. However, it remains unknown how they Here we report that FliP forms homohexameric ring with diameter 10 nm. Alanine substitutions conserved Phe-137, Phe-150, Glu-178 residues periplasmic domain...
Journal Article CryoTEM with a Cold Field Emission Gun That Moves Structural Biology into New Stage Get access Takayuki Kato, Kato Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan Search for other works by this author on: Oxford Academic Google Scholar Fumiaki Makino, Makino Takanori Nakane, Nakane MRC Laboratory Molecular Biology, Cambridge, UK Naoya Terahara, Terahara Takeshi Kaneko, Kaneko JEOL, Akishima, Tokyo, Yuko Shimizu, Shimizu Sohei Motoki, Motoki...
Abstract The bacterial flagellar MS ring is a transmembrane complex acting as the core of motor and template for assembly. C attached to involved in torque generation rotation switch, large symmetry mismatch between these two rings has been long puzzle, especially with respect their role function. Here, using cryoEM structural analysis basal body formed by full-length FliF from Salmonella enterica , we show that native 34 subunits no variation. Symmetry shows variation peak at 34-fold,...
Abstract The basal body of the bacterial flagellum is a rotary motor that consists several rings (C, MS and LP) rod. LP ring acts as bushing supporting distal rod for its rapid stable rotation without much friction. Here, we use electron cryomicroscopy to describe structure around rod, at 3.5 Å resolution, from Salmonella Typhimurium. shows 26-fold rotational symmetry intricate intersubunit interactions each subunit with up six partners, which explains structural stability. inner surface...
Abstract Functionalization of graphene is one the most important fundamental technologies in a wide variety fields including industry and biochemistry. We have successfully achieved novel oxidative modification using photoactivated ClO 2 · as mild oxidant confirmed oxidized grid storable with its functionality for at least three months under N atmosphere. Subsequent chemical functionalization enabled us to develop an epoxidized (EG-grid™), which effectively adsorbs protein particles electron...
The efficient utilization of biomass fuels is a critical component sustainable energy economy. Via respiration, acetic acid bacteria can oxidize ethanol into using membrane-bound alcohol and aldehyde dehydrogenases (ADH AlDH, respectively). Focusing on the ability these enzymes to interact directly electrically with electrode materials, we constructed mediatorless bioanode for oxidation based direct electron transfer (DET)-type bienzymatic cascade by ADH AlDH. three-dimensional structural...
The FliPQR complex constitutes a channel for export of the bacterial flagellar proteins involved in axial structure assembly. It also serves as template rod A periplasmic gate, formed by N-terminal α-helices FliP and FliR, remains closed until FliE assembles onto FliR. mechanism which opens gate has remained unclear. Here, we present cryoEM form at 3.0 Å resolution. β-cap β-strands FliR creates tight seal gate. Interaction with induces conformational change their move up outward....
In this study, we investigated (1) the functional role of large-conductance Ca2+-activated K+ (BKCa) channels in regulation guinea pig vas deferens smooth muscle (VDSM) contractions and (2) potential contractile effects 33 physiologically active substances related chemicals that have not been previously reported to contract VDSM. Iberiotoxin (an inhibitor BKCa channels, 10-7 M) was most potent enhancer both noradrenaline (10-5 M)- ATP (10-6 M)-induced among 6 types channel inhibitors....
Mycoplasma pneumoniae and genitalium are bacterial wall-less human pathogens the causative agents of respiratory reproductive tract infections. Infectivity, gliding motility adhesion these mycoplasmas to host cells mediated by orthologous adhesin proteins forming a transmembrane complex that binds sialylated oligosaccharides cell ligands. Here we report cryo-EM structure M. P1 bound Fab fragment monoclonal antibody P1/MCA4, which stops induces detachment motile cells. The epitope P1/MCA4...
view Abstract Citations (114) References (29) Co-Reads Similar Papers Volume Content Graphics Metrics Export Citation NASA/ADS Discovery of a Prominent Cyclotron Absorption Feature from the Transient X-Ray Pulsar X0331+53 Makishima, K. ; Mihara, T. Ishida, M. Ohashi, Sakao, Tashiro, Tsuru, Kii, Makino, F. Murakami, Nagase, Tanaka, Y. Kunieda, H. Tawara, Kitamoto, S. Miyamoto, Yoshida, A. Turner, J. L. A remarkable absorption feature at 28.5 keV, attributable to electron cyclotron resonance,...
Type III secretion systems are found in many Gram-negative bacteria. They activated by contact with eukaryotic cells and inject virulence proteins inside them. Host cell detection requires a protein complex located at the tip of device's external injection needle. The Shigella (TC) is composed IpaD, hydrophilic protein, IpaB, hydrophobic which later forms part pore host membrane. Here we used labelling crosslinking methods to show that TCs from ΔipaB strain contain five IpaD subunits while...
Abstract The Bacterial flagellar hook is a short supercoiled tubular structure made from helical assembly of the protein FlgE. acts as universal joint that connects basal body and filament, smoothly transmits torque generated by rotary motor to filament propeller. In peritrichously flagellated bacteria, allows filaments form bundle behind cell for swimming, fall apart tumbling. Here we report native at 3.6 Å resolution cryoEM single particle image analysis polyhook. atomic model built into...
Flavin adenine dinucleotide-dependent d-fructose dehydrogenase (FDH) from Gluconobacter japonicus NBRC3260, a membrane-bound heterotrimeric flavohemoprotein capable of direct electron transfer (DET)-type bioelectrocatalysis, was investigated the perspective structural biology, bioelectrochemistry, and protein engineering. DET-type reactions offer several benefits in biomimetics (e.g., biofuel cells, bioreactors, biosensors) owing to their mediator-less configuration. FDH provides an intense...
view Abstract Citations (75) References (12) Co-Reads Similar Papers Volume Content Graphics Metrics Export Citation NASA/ADS Discovery of X-ray bursts from AQL X-1. Koyama, K. ; Inoue, H. Makishima, Matsuoka, M. Murakami, T. Oda, Osgawara, Y. Ohashi, Shibazaki, N. Tanaka, Marshall, F. J. Kondo, I. S. Hayakawa, Kunieda, Makino, Masai, Nagase, Tawara, Miyamoto, Tsunemi, Yamashita, Two were observed the recurrent transient Aql X-1 during declining phase a nova-like flare. These exhibit common...
Type III secretion systems (T3SSs) are essential devices in the virulence of many Gram-negative bacterial pathogens. They mediate injection protein effectors from bacteria into eukaryotic host cells to manipulate them during infection. T3SSs involved (vT3SSs) evolutionarily related flagellar export apparatuses (fT3SSs), which for assembly and cell motility. The structure external transmembrane parts both fT3SS vT3SS is increasingly well-defined. However, arrangement their cytoplasmic inner...
Centromeres are specified by sequence-independent epigenetic mechanisms, and the centromere position may drift at each cell cycle, but once this is specified, it not be frequently moved. Currently, unclear whether stable. To address question, we systematically analyzed of nonrepetitive centromeres in 21 independent clones isolated from a laboratory stock chicken DT40 cells using chromatin immunoprecipitation combined with massive parallel sequencing analysis anti-CENP-A antibody. We...
Abstract Photosystem I (PSI) is a light driven electron pump transferring electrons from Cytochrome c 6 (Cyt ) to Ferredoxin (Fd). An understanding of this transfer process hampered by paucity structural detail concerning PSI:Fd interface and the possible binding sites Cyt . Here we describe high resolution cryo-EM structure Thermosynechococcus elongatus BP-1 PSI in complex with Fd loosely bound Side chain interactions at including bridging water molecules are visualized detail. The explains...
Abstract Centromere protein A (CENP‐A) nucleosomes containing the centromere‐specific histone H3 variant CENP‐A represent an epigenetic mark that specifies centromere position. The Mis18 complex is a licensing factor for new deposition via chaperone, Holliday junction recognition (HJURP), on chromatin. Chicken KINETOCHORE NULL2 (KNL2) (ggKNL2), component, has CENP‐C‐like motif, and our previous study suggested ggKNL2 directly binds to nucleosome recruit HJURP/CENP‐A centromere. However,...
Abstract The bacterial flagellar hook connects the helical filament to rotary motor at its base. Bending flexibility of allows filaments form a bundle behind cell body produce thrust for motility. protein FlgE shows considerable sequence and structural similarities distal rod FlgG; however, is supercoiled flexible as universal joint whereas straight rigid drive shaft. A short FlgG specific (GSS) has been postulated confer rigidity on rod, insertion GSS position between Phe-42 Ala-43 actually...
Tungsten-containing formate dehydrogenase from Methylorubrum extroquens AM1 (FoDH1)-a promising biocatalyst for the interconversion of carbon dioxide/formate and nicotine adenine dinucleotide (NAD+)/NADH redox couples-was investigated using structural biology bioelectrochemistry. FoDH1 is reported to be an enzyme that can realize "direct electron transfer (DET)-type bioelectrocatalysis." However, its 3-D structure, electrode-active sites, (ET) pathways remain unclear. The ET were...